scholarly journals MUC1- and Survivin-based DNA Vaccine Combining Immunoadjuvants CpG and interleukin-2 in a Bicistronic Expression Plasmid Generates Specific Immune Responses and Antitumour Effects in a Murine Colorectal Carcinoma Model

2018 ◽  
Vol 87 (2) ◽  
pp. 63-72 ◽  
Author(s):  
C. Liu ◽  
Y. Xie ◽  
B. Sun ◽  
F. Geng ◽  
F. Zhang ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Immune Responses ◽  
Dna Vaccine ◽  
Interleukin 2 ◽  
Expression Plasmid ◽  
Antitumour Effects ◽  
Bicistronic Expression

scholarly journals Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant

2003 ◽  
Vol 52 (3) ◽  
pp. 223-228 ◽  
Author(s):  
Mingzhao Zhu ◽  
Xuemei Xu ◽  
Hongwei Liu ◽  
Xiaojuan Liu ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immune Responses ◽  
Dna Vaccine ◽  
Delayed Type Hypersensitivity ◽  
T Helper 1 ◽  
Expression Plasmid ◽  
Herpes Simplex Virus 1 ◽  
Simplex Virus ◽  
Hsv 1

In this study, the immune-modulatory and vaccine effects of using an interleukin (IL)-18 expression plasmid as a genetic adjuvant to enhance DNA vaccine-induced immune responses were investigated in a mouse herpes simplex virus 1 (HSV-1) challenge model. BALB/c mice were immunized by three intramuscular inoculations of HSV-1 glycoprotein D (gD) DNA vaccine alone or in combination with a plasmid expressing mature IL-18 peptide. Both the serum IgG2a/IgG1 ratio and T helper 1-type (Th1) cytokines [IL-2 and interferon (IFN)-γ] were increased significantly by the co-injection of the IL-18 plasmid compared with the injection of gD DNA alone. However, the production of IL-10 was inhibited by IL-18 plasmid co-injection. Furthermore, IL-18 plasmid co-injection efficiently enhanced antigen-specific lymphocyte proliferation and the delayed-type hypersensitivity response. When mice were challenged with HSV-1 at the cornea, co-injection of IL-18 plasmid with gD DNA vaccine showed significantly better protection, manifested as lower corneal lesion scores and faster recovery. These experiments indicate that co-injection of an IL-18 plasmid with gD DNA vaccine efficiently induces Th1-dominant immune responses and improves the protective effect against HSV-1 infection.

scholarly journals A BCR/ABL-hIL-2 DNA Vaccine Enhances the Immune Responses in BALB/c Mice

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yanan Qin ◽  
Hongxia Tian ◽  
Guanming Wang ◽  
Chen Lin ◽  
Yangqiu Li
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Residual Disease ◽  
Fusion Gene ◽  
Interleukin 2 ◽  
K562 Cells ◽  
Serum Levels ◽  
Muscle Tissues ◽  
Cellular Immune

The use of a DNA vaccine encoding the BCR/ABL fusion gene is thought to be a promising approach for patients with chronic myeloid leukemia (CML) to eradicate minimal residual disease after treatment with chemotherapy or targeted therapy. In this study, our strategy employs genetic technology to create a DNA vaccine encoding the BCR/ABL fusion and human interleukin-2 (hIL-2) genes. The successfully constructed plasmids BCR/ABL-pIRES-hIL-2, BCR/ABL-pIRES, and pIRES-hIL-2 were delivered intramuscularly to BALB/c mice at 14-day intervals for three cycles. The transcription and expression of the BCR/ABL and hIL-2 genes were found in the injected muscle tissues. The interferon-γ(IFN-γ) serum levels were increased, and the splenic CD4+/CD8+T cell ratio was significantly decreased in the BCR/ABL-pIRES-hIL-2-injected mice. Furthermore, specific antibodies against K562 cells could be detected by indirect immunofluorescence. These results indicate that a DNA vaccine containing BCR/ABL and hIL-2 together may elicit increased in vivo humoral and cellular immune responses in BALB/c mice.

scholarly journals Modulation of Antigen-Specific Humoral Responses in Rhesus Macaques by Using Cytokine cDNAs as DNA Vaccine Adjuvants

2000 ◽  
Vol 74 (7) ◽  
pp. 3427-3429 ◽  
Author(s):  
Jong J. Kim ◽  
Joo-Sung Yang ◽  
Thomas C. VanCott ◽  
Daniel J. Lee ◽  
Kelledy H. Manson ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Rhesus Macaques ◽  
Interleukin 2 ◽  
Dna Immunization ◽  
Vaccine Adjuvants ◽  
Vaccine Strategy ◽  
Humoral Immune ◽  
Macaque Model ◽  
Immunodeficiency Virus

ABSTRACT An important limitation of DNA immunization in nonhuman primates is the difficulty in generating high levels of antigen-specific antibody responses; strategies to enhance the level of immune responses to DNA immunization may be important in the further development of this vaccine strategy for humans. We approached this issue by testing the ability of molecular adjuvants to enhance the levels of immune responses generated by multicomponent DNA vaccines in rhesus macaques. Rhesus macaques were coimmunized intramuscularly with expression plasmids bearing genes encoding Th1 (interleukin 2 [IL-2] and gamma interferon)- or Th2 (IL-4)-type cytokines and DNA vaccine constructs encoding human immunodeficiency virus Env and Rev and simian immunodeficiency virus Gag and Pol proteins. We observed that the cytokine gene adjuvants (especially IL-2 and IL-4) significantly enhanced antigen-specific humoral immune responses in the rhesus macaque model. These results support the assumption that antigen-specific responses can be engineered to a higher and presumably more desirable level in rhesus macaques by genetic adjuvants.

scholarly journals Induction of Specific Immune Responses by Severe Acute Respiratory Syndrome Coronavirus Spike DNA Vaccine with or without Interleukin-2 Immunization Using Different Vaccination Routes in Mice

2007 ◽  
Vol 14 (7) ◽  
pp. 894-901 ◽  
Author(s):  
Hui Hu ◽  
Xinya Lu ◽  
Ling Tao ◽  
Bingke Bai ◽  
Zhenfeng Zhang ◽  
...  
Keyword(s):  
T Cell ◽  
Severe Acute Respiratory Syndrome ◽  
Immune Responses ◽  
Antibody Response ◽  
Dna Vaccine ◽  
Cell Response ◽  
Interleukin 2 ◽  
T Cell Response ◽  
Cellular Immune Responses ◽  
Cellular Immune

ABSTRACT DNA vaccines induce humoral and cellular immune responses in animal models and humans. To analyze the immunogenicity of the severe acute respiratory syndrome (SARS) coronavirus (CoV), SARS-CoV, spike DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2), DNA vaccine plasmids pcDNA-S and pcDNA-IL-2 were constructed and inoculated into BALB/c mice with or without pcDNA-IL-2 by using three different immunization routes (the intramuscular route, electroporation, or the oral route with live attenuated Salmonella enterica serovar Typhimurium). The cellular and humoral immune responses were assessed by enzyme-linked immunosorbent assays, lymphocyte proliferation assays, enzyme-linked immunospot assays, and fluorescence-activated cell sorter analyses. The results showed that specific humoral and cellular immunities could be induced in mice by inoculating them with SARS-CoV spike DNA vaccine alone or by coinoculation with IL-2-expressing plasmids. In addition, the immune response levels in the coinoculation groups were significantly higher than those in groups receiving the spike DNA vaccine alone. The comparison between the three vaccination routes indicated that oral vaccination evoked a vigorous T-cell response and a weak response predominantly with subclass immunoglobulin G2a (IgG2a) antibody. However, intramuscular immunization evoked a vigorous antibody response and a weak T-cell response, and vaccination by electroporation evoked a vigorous response with a predominant subclass IgG1 antibody response and a moderate T-cell response. Our findings show that the spike DNA vaccine has good immunogenicity and can induce specific humoral and cellular immunities in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances the humoral and cellular immune responses. Different vaccination routes also evoke distinct immune responses. This study provides basic information for the design of DNA vaccines against SARS-CoV.

scholarly journals Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice

2009 ◽  
Vol 31 (11) ◽  
Author(s):  
Hui Hu ◽  
Ling Tao ◽  
Yabin Wang ◽  
Liying Chen ◽  
Jihong Yang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Interleukin 2

SIV DNA vaccine co-administered with IL-12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques

2005 ◽  
Vol 34 (5-6) ◽  
pp. 262-270 ◽  
Author(s):  
Jean D. Boyer ◽  
Tara M. Robinson ◽  
Michele A. Kutzler ◽  
R. Parkinson ◽  
Sandra A. Calarota ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Expression Plasmid ◽  
Cellular Immune Responses ◽  
Cynomolgus Macaques ◽  
Cellular Immune
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