scholarly journals P5‐87: Time course of oxygen demand in patients with COVID‐19 pneumonia

Respirology ◽  
2021 ◽  
Vol 26 (S3) ◽  
pp. 191-192
Keyword(s):  
Time Course ◽  
Oxygen Demand

Apparent diffusion time of oxygen from blood to tissue in rat cerebral cortex: implication for tissue oxygen dynamics during brain functions

2007 ◽  
Vol 103 (4) ◽  
pp. 1352-1358 ◽  
Author(s):  
Kazuto Masamoto ◽  
Jeff Kershaw ◽  
Masakatsu Ureshi ◽  
Naosada Takizawa ◽  
Hirosuke Kobayashi ◽  
...  
Keyword(s):  
Time Course ◽  
Oxygen Demand ◽  
Excess Demand ◽  
Tissue Oxygen ◽  
Doppler Flowmetry ◽  
Demand And Supply ◽  
Brain Functions ◽  
Apparent Diffusion ◽  
Time Required ◽  
Stimulus Length

To investigate the dynamics of tissue oxygen demand and supply during brain functions, we simultaneously recorded Po2 and local cerebral blood flow (LCBF) with an oxygen microelectrode and laser Doppler flowmetry, respectively, in rat somatosensory cortex. Electrical hindlimb stimuli were applied for 1, 2, and 5 s to vary the duration of evoked cerebral metabolic rate of oxygen (CMRO2). The electrical stimulation induced a robust increase in Po2 (4–9 Torr at peak) after an increase in LCBF (14–26% at peak). A consistent lag of ∼1.2 s (0.6–2.3 s for individual animals) in the Po2 relative to LCBF was found, irrespective of stimulus length. It is argued that the lag in Po2 was predominantly caused by the time required for oxygen to diffuse through tissue. During brain functions, the supply of fresh oxygen further lagged because of the latency of LCBF onset (∼0.4 s). The results indicate that the tissue oxygen supports excess demand until the arrival of fresh oxygen. However, a large drop in Po2 was not observed, indicating that the evoked neural activity demands little extra oxygen or that the time course of excess demand is as slow as the increase in supply. Thus the dynamics of Po2 during brain functions predominantly depend on the time course of LCBF. Possible factors influencing the lag between demand and supply are discussed, including vascular spacing, reactivity of the vessels, and diffusivity of oxygen.

scholarly journals Composting of mushroom substrate in a fermentation tunnel: compost parameters and a mathematical model

1994 ◽  
Vol 42 (4) ◽  
pp. 271-292 ◽  
Author(s):  
J.J.C. Van Lier ◽  
J.T. Van Ginkel ◽  
G. Straatsma ◽  
J.P.G. Gerrits ◽  
L.J.L.D. Van Griensven
Keyword(s):  
Mathematical Model ◽  
Time Course ◽  
Calculated Data ◽  
Oxygen Demand ◽  
Conductive Heat ◽  
Actual Process ◽  
Process Data ◽  
Continuous Simulation ◽  
Weight Losses ◽  
Air Stream

Phase II of composting of mushroom substrate was studied in bulk fermentation tunnels. Compost data are given on heat production, settling and mass reduction, porosity and thermal conductivity. Mass and moisture determinations at the end of the process indicated slightly positive gradients in the direction of the air stream. The highest rate of degradation occurred during the first 2 days. A mathematical model of mass and heat transfers was devised. Differential equations were solved with time-dependent analysis using a Continuous Simulation and Modelling Program (CSMP). In the calculations, the substrate was divided into theoretical layers of equal thickness but of different density and porosity. The model predicts the time-course of the process taking into account the moisture content and the filling height of the compost, and the amounts of supplied fresh air and recirculated air. The calculated data include the oxygen demand, the water and dry matter losses, the temperatures in the various layers, and the loss of conductive heat through the walls of the containers in the tunnel. Calculated data corresponded with actual process data. Temp. were correct within 3 degrees C and weight losses within 5%.

Investigation of UV-assisted chlorophenol congeners’ degradation by organic oxidant p-nitrobenzoic acid in basic media

2013 ◽  
Vol 67 (11) ◽  
pp. 2418-2427 ◽  
Author(s):  
Swati Sharma ◽  
Mausumi Mukhopadhyay ◽  
Zagabathuni Venkata Panchakshari Murthy
Keyword(s):  
Time Course ◽  
Degradation Kinetics ◽  
Oxygen Demand ◽  
Chloride Ions ◽  
Ring Cleavage ◽  
Spectroscopy Analysis ◽  
Residual Concentration ◽  
Nitrobenzoic Acid ◽  
Organic Oxidant ◽  
Gas Chromatography Mass Spectroscopy

This research specifically addressed the photodegradation of selected model chlorophenol (CP) congeners, 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP), by an organic oxidant, p-nitrobenzoic acid (PNBA), in basic media. The reactions were followed by high-pressure liquid chromatography and gas chromatography–mass spectroscopy analysis for residual concentration and the identification of photodegraded products respectively. Initial degradation was confirmed by the formation of ring cleavage compounds, and subsequent mineralization was evidently marked by an increase in release of chloride ions in the time course profile. The presence of fewer/no chlorine atoms in the degraded compounds corroborated the mineralization by UV/PNBA action. The chemical oxygen demand (COD) was also observed to fall appreciably to the extent of 73% during all CP congeners’ degradation. The maximum COD decrease was observed in PNBA-assisted 4-CP degradation. The overall degradation kinetics conforming to second-order reaction is observed to follow the order 4-CP > 2,4,6-TCP ∼ PCP ∼ 2,4-DCP. The findings of this investigation elucidated the fact that PNBA was capable of successfully degrading/mineralizing the selected CP congeners.

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

Destruction of Actin Filaments by Osmium Tetroxide

1977 ◽  
Vol 35 ◽  
pp. 466-467
Author(s):  
P. Maupin-Szamier ◽  
T. D. Pollard
Keyword(s):  
Actin Filaments ◽  
Time Course ◽  
Osmium Tetroxide ◽  
Rabbit Muscle ◽  
Muscle Actin ◽  
Sodium Phosphate Buffer ◽  
Transmission Electron ◽  
The Difference ◽  
Rates Of Decay ◽  
Short Time

We have studied the destruction of rabbit muscle actin filaments by osmium tetroxide (OSO4) to develop methods which will preserve the structure of actin filaments during preparation for transmission electron microscopy.Negatively stained F-actin, which appears as smooth, gently curved filaments in control samples (Fig. 1a), acquire an angular, distorted profile and break into progressively shorter pieces after exposure to OSO4 (Fig. 1b,c). We followed the time course of the reaction with viscometry since it is a simple, quantitative method to assess filament integrity. The difference in rates of decay in viscosity of polymerized actin solutions after the addition of four concentrations of OSO4 is illustrated in Fig. 2. Viscometry indicated that the rate of actin filament destruction is also dependent upon temperature, buffer type, buffer concentration, and pH, and requires the continued presence of OSO4. The conditions most favorable to filament preservation are fixation in a low concentration of OSO4 for a short time at 0°C in 100mM sodium phosphate buffer, pH 6.0.

The time course of calcium deposition in shells of the barnacle (Balanus amphititre amphititre) during cyprid-juvenile metamorphosis

1994 ◽  
Vol 52 ◽  
pp. 178-179
Author(s):  
Nancy R. Wallace ◽  
Craig C. Freudenrich ◽  
Karl Wilbur ◽  
Peter Ingram ◽  
Ann LeFurgey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Time Course ◽  
Vertical Plane ◽  
Mantle Cavity ◽  
Qualitative Assessment ◽  
Calcium Deposition ◽  
Free Swimming ◽  
Freeze Dried ◽  
Scanning Electron

The morphology of balanomorph barnacles during metamorphosis from the cyprid larval stage to the juvenile has been examined by light microscopy and scanning electron microscopy (SEM). The free-swimming cyprid attaches to a substrate, rotates 90° in the vertical plane, molts, and assumes the adult shape. The resulting metamorph is clad in soft cuticle and has an adult-like appearance with a mantle cavity, thorax with cirri, and incipient shell plates. At some time during the development from cyprid to juvenile, the barnacle begins to mineralize its shell, but it is not known whether calcification occurs before, during, or after ecdysis. To examine this issue, electron probe x-ray microanalysis (EPXMA) was used to detect calcium in cyprids and juveniles at various times during metamorphosis.Laboratory-raised, free-swimming cyprid larvae were allowed to settle on plastic coverslips in culture dishes of seawater. The cyprids were observed with a dissecting microscope, cryopreserved in liquid nitrogen-cooled liquid propane at various times (0-24 h) during metamorphosis, freeze dried, rotary carbon-coated, and examined with scanning electron microscopy (SEM). EPXMA dot maps were obtained in parallel for qualitative assessment of calcium and other elements in the carapace, wall, and opercular plates.

Arabidopsis 4-COUMAROYL-COA LIGASE 8 contributes to the biosynthesis of the benzenoid ring of coenzyme Q in peroxisomes

2019 ◽  
Vol 476 (22) ◽  
pp. 3521-3532
Author(s):  
Eric Soubeyrand ◽  
Megan Kelly ◽  
Shea A. Keene ◽  
Ann C. Bernert ◽  
Scott Latimer ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Time Course ◽  
Reverse Genetics ◽  
De Novo ◽  
Coenzyme Q ◽  
Control Level ◽  
Wild Type ◽  
Fluorescent Reporters ◽  
Coa Ligase ◽  
Peroxisomal Targeting

Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE-COA LIGASE 8 (4-CL8; At5g38120) as a contributor to the β-oxidation of p-coumarate for ubiquinone biosynthesis. The enzyme is part of the same clade (V) of acyl-activating enzymes than At4g19010, a p-coumarate CoA ligase known to play a central role in the conversion of p-coumarate into 4-hydroxybenzoate. A 4-cl8 T-DNA knockout displayed a 20% decrease in ubiquinone content compared with wild-type plants, while 4-CL8 overexpression boosted ubiquinone content up to 150% of the control level. Similarly, the isotopic enrichment of ubiquinone's ring was decreased by 28% in the 4-cl8 knockout as compared with wild-type controls when Phe-[Ring-13C6] was fed to the plants. This metabolic blockage could be bypassed via the exogenous supply of 4-hydroxybenzoate, the product of p-coumarate β-oxidation. Arabidopsis 4-CL8 displays a canonical peroxisomal targeting sequence type 1, and confocal microscopy experiments using fused fluorescent reporters demonstrated that this enzyme is imported into peroxisomes. Time course feeding assays using Phe-[Ring-13C6] in a series of Arabidopsis single and double knockouts blocked in the β-oxidative metabolism of p-coumarate (4-cl8; at4g19010; at4g19010 × 4-cl8), flavonol biosynthesis (flavanone-3-hydroxylase), or both (at4g19010 × flavanone-3-hydroxylase) indicated that continuous high light treatments (500 µE m−2 s−1; 24 h) markedly stimulated the de novo biosynthesis of ubiquinone independently of kaempferol catabolism.

Short-term volume and turgor regulation in yeast

2008 ◽  
Vol 45 ◽  
pp. 147-160 ◽  
Author(s):  
Jörg Schaber ◽  
Edda Klipp
Keyword(s):  
Dynamic Model ◽  
Volume Change ◽  
Volume Regulation ◽  
Time Course ◽  
Osmotic Shock ◽  
Intracellular Concentration ◽  
Short Term ◽  
Hydrodynamic Property ◽  
Turgor Regulation ◽  
Thermodynamic Framework

Volume is a highly regulated property of cells, because it critically affects intracellular concentration. In the present chapter, we focus on the short-term volume regulation in yeast as a consequence of a shift in extracellular osmotic conditions. We review a basic thermodynamic framework to model volume and solute flows. In addition, we try to select a model for turgor, which is an important hydrodynamic property, especially in walled cells. Finally, we demonstrate the validity of the presented approach by fitting the dynamic model to a time course of volume change upon osmotic shock in yeast.

Preliminary Evaluation of a Weibull Function for Fitting Slow-Component Eye Velocity over the Time Course of Caloric-Induced Nystagmus

1989 ◽  
Vol 32 (3) ◽  
pp. 681-687 ◽  
Author(s):  
C. Formby ◽  
B. Albritton ◽  
I. M. Rivera
Keyword(s):  
Time Course ◽  
Slow Component ◽  
Weibull Function ◽  
Preliminary Evaluation ◽  
Mathematical Function ◽  
Before And After ◽  
Best Fitting ◽  
Eye Velocity ◽  
Fitting Parameters ◽  
Weibull Equation

We describe preliminary attempts to fit a mathematical function to the slow-component eye velocity (SCV) over the time course of caloric-induced nystagmus. Initially, we consider a Weibull equation with three parameters. These parameters are estimated by a least-squares procedure to fit digitized SCV data. We present examples of SCV data and fitted curves to show how adjustments in the parameters of the model affect the fitted curve. The best fitting parameters are presented for curves fit to 120 warm caloric responses. The fitting parameters and the efficacy of the fitted curves are compared before and after the SCV data were smoothed to reduce response variability. We also consider a more flexible four-parameter Weibull equation that, for 98% of the smoothed caloric responses, yields fits that describe the data more precisely than a line through the mean. Finally, we consider advantages and problems in fitting the Weibull function to caloric data.

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