scholarly journals P2‐51: Action of Panton‐Valentine leukocidin on rabbit alveolar macrophages

Respirology ◽  
2021 ◽  
Vol 26 (S3) ◽  
pp. 133-133
Keyword(s):  
Alveolar Macrophages ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Effect of recombinant Panton–Valentine leukocidin in vitro on apoptosis and cytokine production of human alveolar macrophages

2010 ◽  
Vol 56 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Benquan Wu ◽  
Wenxian Zhang ◽  
Jing Huang ◽  
Hui Liu ◽  
Tiantuo Zhang
Keyword(s):  
Alveolar Macrophages ◽  
Cytokine Production ◽  
Lavage Fluid ◽  
Effector Cells ◽  
Tnf Α ◽  
Human Alveolar Macrophages ◽  
The Impact ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Panton–Valentine leukocidin (PVL) is associated with rare cases of necrotizing pneumonia that occur in otherwise healthy individuals. Human alveolar macrophages (HAMs) are major effector cells in host defense against infections. However, the impact of PVL on HAMs is uncertain. We evaluated the role of PVL in cytotoxicity and production of inflammatory cytokines secreted by HAMs. HAMs were purified from bronchoalveolar lavage fluid. Recombinant PVL (rPVL) was used in the study to interfere with HAM apoptosis and cytokine production in vitro. Hoechst 33342 fluorescence staining, transmission electron microscopy examination, and flow cytometry indicated that rPVL (10 nmol/L) treatment resulted in HAMs with markedly apoptotic characteristics, and HAMs treated with rPVL at 100 nmol/L showed clear indication of necrosis. A treatment of rPVL at 10 nmol/L elicited the secretion of IL-10 by HAMs relative to untreated control cells, but there was a slight decrease in the constitutive secretion of tumor necrosis factor (TNF)-α. Our results indicate that PVL-treated samples decreased HAM viability, leading to apoptosis at low concentrations and necrosis at high concentrations. In addition, PVL-treated cells released increased amounts of IL-10 and decreased amounts of TNF-α under apoptosis-inducing concentrations. Therefore, we speculated that PVL could play a negative role in HAM function at lower concentrations.

Infection by Panton–Valentine leukocidin-producing Staphylococcus aureus clinically mimicking Lemierre's syndrome

2008 ◽  
Vol 57 (1) ◽  
pp. 118-120 ◽  
Author(s):  
Girish H. Shivashankar ◽  
Nishanth Murukesh ◽  
M. P. S. Varma ◽  
Ikram M. Sharif ◽  
Gerard Glynn
Keyword(s):  
Staphylococcus Aureus ◽  
Lemierre’S Syndrome ◽  
Lemierre's Syndrome ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin
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