scholarly journals P2‐41: Porphyromonas gingivalis gingipains potentially affects MUC5AC gene expression and protein levels in respiratory epithelial cells

Respirology ◽  
2021 ◽  
Vol 26 (S3) ◽  
pp. 129-129
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Respiratory Epithelial Cells ◽  
Protein Levels ◽  
Respiratory Epithelial

Cell-specific involvement of HNF-1β in α1-antitrypsin gene expression in human respiratory epithelial cells

2002 ◽  
Vol 282 (4) ◽  
pp. L757-L765 ◽  
Author(s):  
Chaobin Hu ◽  
David H. Perlmutter
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Cell Line ◽  
Primary Cultures ◽  
Synergistic Action ◽  
Intestinal Epithelial ◽  
Hep G2 ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

The synergistic action of hepatocyte nuclear factor (HNF)-1α and HNF-4 plays an important role in expression of the α1-antitrypsin (α1-AT) gene in human hepatic and intestinal epithelial cells. Recent studies have indicated that the α1-AT gene is also expressed in human pulmonary alveolar epithelial cells, a potentially important local site of the lung antiprotease defense. In this study, we examined the possibility that α1-AT gene expression in a human pulmonary epithelial cell line H441 was also directed by the synergistic action of HNF-1α and HNF-4 and/or by the action of HNF-3, which has been shown to play a dominant role in gene expression in H441 cells. The results show that α1-AT gene expression in H441 cells is predominantly driven by HNF-1β, even though HNF-1β has no effect on α1-AT gene expression in human hepatic Hep G2 and human intestinal epithelial Caco-2 cell lines. Expression of α1-AT and HNF-1β was also demonstrated in primary cultures of human respiratory epithelial cells. HNF-4 has no effect on α1-AT gene expression in H441 cells, even when it is cotransfected with HNF-1β or HNF-1α. HNF-3 by itself has little effect on α1-AT gene expression in H441, Hep G2, or Caco-2 cells but tends to have an upregulating effect when cotransfected with HNF-1 in Hep G2 and Caco-2 cells. These results indicate the unique involvement of HNF-1β in α1-AT gene expression in a cell line and primary cultures derived from human respiratory epithelium.

Effect of Mycoplasma pneumoniae Lysate on Interleukin-8 Gene Expression in Human Respiratory Epithelial Cells

CHEST Journal ◽  
2005 ◽  
Vol 128 (1) ◽  
pp. 322-326 ◽  
Author(s):  
Myung Hyun Sohn ◽  
Kyung Eun Lee ◽  
Sung Yon Choi ◽  
Byoung Chul Kwon ◽  
Myung Woong Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Mycoplasma Pneumoniae ◽  
Interleukin 8 ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial
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