scholarly journals BMPR2‐expressing bone marrow‐derived endothelial‐like progenitor cells alleviate pulmonary arterial hypertension in vivo

Respirology ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 1095-1103 ◽  
Author(s):  
Rebecca L. Harper ◽  
Suzanne Maiolo ◽  
Rebekah J. Ward ◽  
Jemma Seyfang ◽  
Michaelia P. Cockshell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Progenitor Cells ◽  
Pulmonary Arterial

scholarly journals Rescue of Monocrotaline-Induced Pulmonary Arterial Hypertension Using Bone Marrow–Derived Endothelial-Like Progenitor Cells

2005 ◽  
Vol 96 (4) ◽  
pp. 442-450 ◽  
Author(s):  
Yidan D. Zhao ◽  
David W. Courtman ◽  
Yupu Deng ◽  
Lakshmi Kugathasan ◽  
Qiuwang Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Progenitor Cells ◽  
Pulmonary Arterial

scholarly journals Bone Marrow-Derived Endothelial Progenitor Cells Contribute to Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats via Inhibition of Store-Operated Ca2+ Channels

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Ran Miao ◽  
Jun Wan ◽  
Jie Liu ◽  
Jason X.-J. Yuan ◽  
Jing Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Right Ventricular ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Control Group ◽  
Trpc Channels ◽  
Endothelial Progenitor ◽  
Pulmonary Arterial

Purpose. This study aimed to explore whether bone marrow- (BM-) derived endothelial progenitor cells (EPCs) contributing to monocrotaline- (MCT-) induced pulmonary arterial hypertension (PAH) in rats via modulating store-operated Ca2+ channels (SOC). Methods. Sprague Dawley (SD) rats were assigned into MCT group (n = 30) and control group (n = 20). Rats in MCT group were subcutaneously administered with 60 mg/kg MCT solution, and rats in control group were injected with equal amount of vehicle. After 3 weeks of treatment, right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index (RVHI) of two groups were measured, and BM-derived EPCs were isolated. Immunochemistry identification and vasculogenesis detection of EPCs were then performed. Ca2+cyt measurement was performed to detect store-operated calcium entry (SOCE) in two groups, followed by determination of Orai and canonical transient receptor potential (TRPC) channels expression. Results. After 3 weeks of treatment, there were significant increases in RVSP and RVHI in MCT group compared with control group, indicating that MCT successfully induced PAH in rats. Moreover, the SOCE (Ca2+cyt rise) in BM-derived EPCs of MCT group was lower than that of control group. Furthermore, the expression levels of Orai3, TRPC1, TRPC3, and TRPC6 in BM-derived EPCs were decreased in MCT group in comparison with control group. Conclusions. The SOC activities were inhibited in BM-derived EPCs of MCT-treated rats. These results may be associated with the depressed expression of Orai3, TRPC1, TRPC3, and TRPC6, which are major mediators of SOC.

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