Retrospective analysis of commercial heterospermic and homospermic cooled boar semen: effect of the season, sample type and shipping temperature on sperm quality

2021 ◽  
Author(s):  
Raul Gonzalez‐Castro ◽  
Carolina Porflidt ◽  
Toni Patton ◽  
Donna Goins ◽  
Lisa Herickhoff
Keyword(s):  
Retrospective Analysis ◽  
Sperm Quality ◽  
Sample Type ◽  
Boar Semen

scholarly journals Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 719 ◽  
Author(s):  
Tianzeng Song ◽  
Yi Shi ◽  
Yangang Wang ◽  
Izhar Hyder Qazi ◽  
Christiana Angel ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Storage Conditions ◽  
Potential Implication ◽  
Liquid Storage ◽  
Boar Sperm ◽  
Tlr4 Agonist ◽  
Reasonable Evidence ◽  
Boar Semen ◽  
Induced Apoptosis ◽  
Apoptosis Inhibitor

Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 ± 1.55 EU/mL) following 120 h storage. The addition of 100 μg/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 °C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 °C) and at 37 °C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.

The use of butylated hydroxytoluene in cryopreservation of boar semen

2016 ◽  
Vol 12 (2) ◽  
pp. 21-28
Author(s):  
Monika Trzcińska ◽  
Magdalena Baryła
Keyword(s):  
Dna Fragmentation ◽  
Sperm Quality ◽  
Annexin V ◽  
Egg Yolk ◽  
Butylated Hydroxytoluene ◽  
Preimplantation Embryos ◽  
Progressive Motility ◽  
Boar Semen ◽  
Boar Spermatozoa

The objective of the study was to determine the effect of butylated hydroxytoluene (BHT) on the quality and fertilizing capacity of frozen-thawed (FT) boar semen. Semen from five boars (36 ejaculates) was resuspended in lactose-egg yolk-glycerol extender supplemented with 0 (control), 1.0 (R1), 1.5 (R2) or 2.0 mM BHT (R3). Sperm quality was assessed based on motility (CASA; TM: total motility; PM: progressive motility), phosphatidylserine (PS) translocation across the plasma membrane (Annexin-V-FLuos Staining Kit) and DNA fragmentation (TUNEL Assay). The FT semen was also used for intrauterine artificial insemination (AI) of synchronized gilts. The fertilizing capacity of the FT semen was assessed on the basis of the gilt insemination rate and the number of morphologically normal embryos. The quality of the preimplantation embryos was determined by observing a TUNEL-positive reaction. The highest percentage of progressive motile and viable spermatozoa was noted in extender R3 (74.8 ±4.4% and 63.7 ±5.8%), as compared with the control (38.3 ±2.8% and 36.1 ±2.6%). The addition of BHT to the extender did not increase early apoptotic changes in the frozen-thawed spermatozoa with respect to the control. Irrespective of the variant of the extender, cryopreservation and thawing did not induce fragmentation in the boar spermatozoa. The highest number of morphologically normal embryos from inseminated gilts was observed in the case of semen cryopreserved in extender supplemented with 1.5 mM BHT. No significant differences were observed in DNA fragmentation in the expanded blastocysts from gilts inseminated with FT semen cryopreserved in the extenders analysed.

Sperm quality and oxidative status as affected by homogenization of liquid-stored boar semen diluted in short- and long-term extenders

2017 ◽  
Vol 179 ◽  
pp. 67-79 ◽  
Author(s):  
Mariana B. Menegat ◽  
Ana Paula G. Mellagi ◽  
Rafael C. Bortolin ◽  
Tila A. Menezes ◽  
Amanda R. Vargas ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Oxidative Status ◽  
Short And Long Term ◽  
Boar Semen

The benefits of cooling boar semen in long-term extenders prior to cryopreservation on sperm quality characteristics

2016 ◽  
Vol 51 (5) ◽  
pp. 781-788 ◽  
Author(s):  
K Wasilewska ◽  
Ł Zasiadczyk ◽  
L Fraser ◽  
M Mogielnicka-Brzozowska ◽  
W Kordan
Keyword(s):  
Sperm Quality ◽  
Quality Characteristics ◽  
Boar Semen

scholarly journals Assessment of sperm quality traits in relation to fertility in boar semen

2009 ◽  
Vol 51 (1) ◽  
Author(s):  
Neringa Sutkeviciene ◽  
Vita Riskeviciene ◽  
Aloyzas Januskauskas ◽  
Henrikas Zilinskas ◽  
Magnus Andersson
Keyword(s):  
Sperm Quality ◽  
Quality Traits ◽  
Boar Semen

scholarly journals Changes in sperm quality and lipid composition during cryopreservation of boar semen

Reproduction ◽  
2001 ◽  
pp. 395-401 ◽  
Author(s):  
S Cerolini ◽  
A Maldjian ◽  
F Pizzi ◽  
TM Gliozzi
Keyword(s):  
Lipid Content ◽  
Free Cholesterol ◽  
Sperm Quality ◽  
Cholesterol Content ◽  
Sperm Viability ◽  
Freezing And Thawing ◽  
Motile Cells ◽  
Boar Semen ◽  
Thawing Procedure ◽  
Fresh Semen

The effect of cryopreservation on boar sperm viability, motility, lipid content and antioxidant enzymatic activities was studied. Three classes of semen were determined according to a cluster analysis on the basis of the proportion of live and dead cells after freezing and thawing. The classes identified were: high (H, n = 4), average (A, n = 12) and low (L, n = 3) viability. The concentration of sperm cells decreased from class H to A to L. Fresh semen samples with higher viability and a higher proportion of motile cells also maintained better quality after the freezing and thawing procedure. Sperm viability and motility in both fresh and thawed samples were similar in classes H and A, while significantly lower values were measured in class L. The relative decrease in sperm viability and motility after cryopreservation increased from class H to A to L. The lipid content of spermatozoa (micrograms per 10(9) cells) increased significantly after freezing and thawing in classes H and A but not in class L. This result indicated that active sperm lipid metabolism might be responsible for the increase in lipid content. Phospholipid and triacylglycerol contents increased whereas free cholesterol content decreased after thawing. The fatty acid composition of fresh spermatozoa was similar in all three classes. The proportion of polyunsaturated fatty acids decreased significantly after freezing and thawing, indicating contamination from the diluent or peroxidation. After freezing and thawing, superoxide dismutase activity in spermatozoa was significantly higher in class L than in classes H and A, which did not differ from each other.

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