Glycine alleviates fluoride‐induced oxidative stress, apoptosis and senescence in a porcine testicular Sertoli cell line

2021 ◽  
Author(s):  
Ying Liu ◽  
Boxing Sun ◽  
Shaoxuan Zhang ◽  
Jing Li ◽  
Jiajia Qi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Sertoli Cell ◽  
Sertoli Cell Line

Establishment and characterization of porcine Sertoli cell line for the study of xenotransplantation

2007 ◽  
Vol 14 (2) ◽  
pp. 112-118 ◽  
Author(s):  
Hak-Mo Lee ◽  
Byoung Chol Oh ◽  
Dong-Pyo Lim ◽  
Dong-Sup Lee ◽  
Jaejin Cho ◽  
...  
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Sertoli Cell Line

Establishment of a Mouse Sertoli Cell Line Producing Rat Androgen-binding Protein (ABP)

Steroids ◽  
1998 ◽  
Vol 63 (5-6) ◽  
pp. 285-287 ◽  
Author(s):  
Angélique Ducray ◽  
Michèle Bloquel ◽  
Ketsia Hess ◽  
Geoffrey L. Hammond ◽  
Hubert Gérard ◽  
...  
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Binding Protein ◽  
Androgen Binding Protein ◽  
Androgen Binding ◽  
Sertoli Cell Line

SOX9 and SF1 are involved in cyclic AMP-mediated upregulation of anti-Müllerian gene expression in the testicular prepubertal Sertoli cell line SMAT1

2011 ◽  
Vol 301 (3) ◽  
pp. E539-E547 ◽  
Author(s):  
Celina Lasala ◽  
Helena F. Schteingart ◽  
Nassim Arouche ◽  
Patricia Bedecarrás ◽  
Romina P. Grinspon ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Cell Line ◽  
Sertoli Cell ◽  
Nuclear Translocation ◽  
Targeted Mutagenesis ◽  
Signaling Cascades ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Alternative Pathways ◽  
Sertoli Cell Line

In Sertoli cells, anti-Müllerian hormone (AMH) expression is upregulated by FSH via cyclic AMP (cAMP), although no classical cAMP response elements exist in the AMH promoter. The response to cAMP involves NF-κB and AP2; however, targeted mutagenesis of their binding sites in the AMH promoter do not completely abolish the response. In this work we assessed whether SOX9, SF1, GATA4, and AP1 might represent alternative pathways involved in cAMP-mediated AMH upregulation, using real-time RT-PCR (qPCR), targeted mutagenesis, luciferase assays, and immunocytochemistry in the Sertoli cell line SMAT1. We also explored the signaling cascades potentially involved. In qPCR experiments, Amh, Sox9, Sf1, and Gata4 mRNA levels increased after SMAT1 cells were incubated with cAMP. Blocking PKA abolished the effect of cAMP on Sox9, Sf1, and Gata4 expression, inhibiting PI3K/PKB impaired the effect on Sf1 and Gata4, and reducing MEK1/2 and p38 MAPK activities curtailed Gata4 increase. SOX9 and SF1 translocated to the nucleus after incubation with cAMP. Mutations of the SOX9 or SF1 sites, but not of GAT4 or AP1 sites, precluded the response of a 3,063-bp AMH promoter to cAMP. In conclusion, in the Sertoli cell line SMAT1 cAMP upregulates SOX9, SF1, and GATA4 expression and induces SOX9 and SF1 nuclear translocation mainly through PKA, although other kinases may also participate. SOX9 and SF1 binding to the AMH promoter is essential to increase the activity of the AMH promoter in response to cAMP.

Physiological effects of 1,25-dihydroxyvitamin D3 in TM4 Sertoli cell line

1992 ◽  
Vol 262 (6) ◽  
pp. E884-E890 ◽  
Author(s):  
V. L. Akerstrom ◽  
M. R. Walters
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Gradient Analysis ◽  
Cell Number ◽  
Receptor Interaction ◽  
Detectable Change ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Dose Dependent ◽  
Sertoli Cell Line

1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] receptors have been previously described in Sertoli cells. This study was performed to assess biological activity of the receptor in the mouse Sertoli cell line TM4. A 2-h preincubation with 0.01-25 nM 1,25(OH)2D3 resulted in a dose-dependent rapid uptake of 45Ca2+ within 5 min of addition of the isotope to the cells (27 +/- 8%, n = 4 experiments; P less than 0.05). This response was specific for 1,25(OH)2D3, in that it was not induced by 25-hydroxyvitamin D3, estradiol, cortisol, R 5020 (promegestone), or testosterone. However, a combination of testosterone and 1,25(OH)2D3 inhibited uptake by 23 +/- 8% (n = 3 experiments, P less than 0.01). That the mechanism responsible for 1,25(OH)2D3-stimulated uptake may involve 1,25(OH)2D3 receptor interaction is supported by the observation that cycloheximide inhibited the response. Conversely, there was no detectable change in uptake by 1,25(OH)2D3-treated cells after 24-h incubation with 0.1-5 nM 1,25(OH)2D3. Increased levels of DNA and protein content also resulted from a 2-h incubation with the steroid and were sustained up to 24 h without a concomitant increase in cell number or a detectable change in cell morphology. The presence of specific 1,25(OH)2D3 receptor-like binding sites was demonstrated by sucrose gradient analysis and hydroxylapatite assay. These data demonstrate that 1,25(OH)2D3 may play an important role in testicular function through regulation of receptor-mediated events.

scholarly journals Sustained Expression of Insulin by a Genetically Engineered Sertoli Cell Line after Allotransplantation in Diabetic BALB/c Mice1

2014 ◽  
Vol 90 (5) ◽  
Author(s):  
Gurvinder Kaur ◽  
Lea Ann Thompson ◽  
Mithun Pasham ◽  
Kim Tessanne ◽  
Charles R. Long ◽  
...  
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Genetically Engineered ◽  
Sertoli Cell Line
Sign in / Sign up

Export Citation Format

Share Document