The effects of luteinizing hormone as a suppression factor for apoptosis in bovine luteal cells in vitro

2021 ◽  
Author(s):  
Anom Bowolaksono ◽  
Muhammad Fauzi ◽  
Ayu Mulia Sundari ◽  
Anantya Pustimbara ◽  
Retno Lestari ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Suppression Factor ◽  
Luteal Cells

Effect of ovine conceptus and endometrial secretory products on luteal progesterone production in vitro

1986 ◽  
Vol 64 (12) ◽  
pp. 1556-1560
Author(s):  
D. W. Beckner ◽  
J. G. Manns
Keyword(s):  
Luteinizing Hormone ◽  
Conditioned Medium ◽  
Endometrial Tissue ◽  
Minimal Essential Medium ◽  
Luteal Cells ◽  
Endometrial Cells ◽  
Progesterone Production ◽  
Luteal Tissue ◽  
Secretory Products

The objectives of this study were the following: (i) to determine if ovine conceptus secretory products are directly luteotrophic to luteal tissue in vitro and (ii) to determine if ovine conceptus secretory products stimulate endometrial tissue to secrete a luteotropin in vitro. Conceptus-conditioned medium (CCM) was prepared by incubating day 14 ovine conceptuses in minimal essential medium (MEM) for 24 h and harvesting the supernatant. Endometrium-conditioned CCM (E-CCM) and endometrium-conditioned medium (ECM) were prepared by incubating dispersed ovine endometrial cells from day 9–10 cycling ewes in CCM or MEM, respectively, for 16 h and harvesting the supernatants. Media, conditioned as described, were incubated at various dilutions with dispersed luteal cells from day 9–10 cycling ewes for 90 min or 6 h in the absence or presence of 50 ng/mL ovine luteinizing hormone (oLH). CCM did not alter progesterone (P4) production in the 90-min incubation but did increase (p < 0.05) P4 production in the 6-h incubation (1:4, 1:8, 1:16 dilutions). When coincubated with oLH, CCM did not increase P4 production above that stimulated by oLH alone. The effect of E-CCM was similar to CCM or ECM and did not differ significantly from basal. It is concluded that the day 14 ovine conceptus does secrete a factor that is able to directly stimulate P4 secretion by luteal cells in a 6-h, but not a 90-min, incubation. Conceptus secretory products did not stimulate endometrial cells to secrete a luteotropin. The data are consistent with the suggestion that the luteotrophic factor secreted by the conceptus is a prostaglandin of the E series.

Effect of melatonin on the in vitro secretion of progesterone and estradiol 17β by ovine granulosa cells

1992 ◽  
Vol 127 (4) ◽  
pp. 366-370 ◽  
Author(s):  
Mario Baratta ◽  
Carlo Tamanini
Keyword(s):  
Luteinizing Hormone ◽  
Granulosa Cells ◽  
Advanced Degree ◽  
Synergistic Activity ◽  
Hormone Production ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Degree Of Differentiation ◽  
Estradiol 17Β

This study was undertaken to determine the effect of melatonin on steroid hormone production by ovine granulosa and luteal cells in vitro. Granulosa and luteal cells from ovine ovaries were cultured for nine days either in D-MEM only or in the presence of melatonin (0.86, 8.6, 86 nmol/l), ovine luteinizing hormone (oLH, 2 μg/l) or a combination of both these hormones. Progesterone (P4) and estradiol 17β (E2) were determined by validated RIAs. Melatonin stimulation began at either day 1 or day 5 of culture. Melatonin (0.86 nmol/l) significantly increased (p<0.001) progesterone secretion by granulosa cells both when administered alone and when administered in combination with oLH; the more marked response was observed in the latter case. When the stimulation began at day 5, at a more advanced degree of differentiation of the cells, higher levels of P4 were observed. Higher concentrations of melatonin did not further increase progesterone production. Melatonin alone did not have a significant effect on the production of estradiol 17β; neither did melatonin stimulate progesterone production in either long-term cultured luteal cells or in short-term (1–2 h) cultured luteal and granulosa cells. The results of this study document a direct effect of melatonin in stimulating granulosa cells to produce progesterone, a synergistic activity between melatonin and luteinizing hormone and a different ability of granulosa cells to secrete P4 depending on the degree of differentiation.

5-Lipoxygenase stimulates luteinizing hormone release in vitro

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S60-S61
Author(s):  
M. PRZYLIPIAK ◽  
L. KIESEL ◽  
A. PRZYLIPIAK ◽  
B. RUNNEBAUM
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Release ◽  
Luteinizing Hormone Release ◽  
Release In Vitro

The effect of phorbol esters on steroidogenesis in vitro by bovine luteal cells

1985 ◽  
Vol 110 (1_Suppla) ◽  
pp. S92-S93 ◽  
Author(s):  
B. BRUNSWIG ◽  
L. T. BUDNIK
Keyword(s):  
Phorbol Esters ◽  
Luteal Cells
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