Effect of aglepristone (RU534) administration during follicular phase on progesterone, estradiol‐17β and LH serum concentrations in bitches

2020 ◽  
Vol 55 (12) ◽  
pp. 1794-1802
Author(s):  
Alessandro Troisi ◽  
Angela Polisca ◽  
Lucia Cardinali ◽  
Riccardo Orlandi ◽  
Gabriele Brecchia ◽  
...  
Keyword(s):  
Follicular Phase ◽  
Serum Concentrations ◽  
Estradiol 17Β

scholarly journals Effects of decreased estradiol-17β on the serum and anterior pituitary IGF-I system in pigs

2005 ◽  
Vol 187 (3) ◽  
pp. 369-378 ◽  
Author(s):  
C K Hilleson-Gayne ◽  
J A Clapper
Keyword(s):  
Anterior Pituitary ◽  
Percentage Increase ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Treatment Groups ◽  
Igf System ◽  
Igf I ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Estradiol 17Β

To further delineate the role of estradiol in the IGF system an experiment was conducted to determine the dosage of the aromatase inhibitor, anastrozole, needed to decreases serum concentrations of estradiol-17β (E2) in maturing boars. A second experiment was conducted to determine if administration of anastrozole to growing boars decreased serum concentrations of E2 and affected components of the serum and anterior pituitary gland (AP) IGF system vs untreated boars and barrows. In Experiment 1, 12 crossbred boars (292 days, 158 kg) were administered either 0, 1 or 10 mg/day anastrozole (n=4/group) beginning on day 1. Blood samples were collected every 7–14 days. Mean serum concentrations of E2 were decreased (P < 0·05) in the 10 mg group vs the 0 and 1 mg groups by day 36; however, no difference (P > 0·05) existed between the 0 and 1 mg groups. In Experiment 2, 24 crossbred boars and 12 barrows (101 days, 44 kg) were stratified by litter to one of three treatment groups (n=12): boars administered 10 mg/day anastrozole, boars administered 0 mg/day, and barrows administered 0 mg/day. Blood samples were collected and pigs were weighed on day 0 and every 14 days thereafter, then killed on day 84 when blood and APs were collected. The 10 mg/day pigs were fed the anastrozole-amended diet beginning on day 1. Mean serum concentrations of E2 did not differ (P > 0·05) between the 10 mg/day pigs and 0 mg/day pigs on day 0; however, on day 15 through to 84 mean serum concentrations of E2 were greater (P < 0·05) in 0 mg/day pigs than in the 10 mg/day pigs. Mean percentage increase in serum concentrations of IGF-I was greater (P < 0·05) in untreated boars than anastrozole-treated boars and barrows from day 58 through to 84. Mean percentage of basal IGF-I increased (P < 0·05) from day 29 through to 84 in untreated boars. Mean relative amounts of AP IGF-binding protein (IGFBP)-2 and -5 were less (P < 0·01) in 10 mg/day pigs than in the 0 mg/day pigs, but each was greater (P < 0·01) than in barrows administered 0 mg/day. These results indicate anastrozole administered at a dosage of 10 mg/day suppresses serum concentrations of E2 in pigs. Administration of anastrozole to boars reduced the percentage increase in serum concentrations of IGF-I and relative amounts of AP IGFBP-2 and -5. These data further support a role for E2 in regulating components of the IGF system in pigs.

scholarly journals Dihydrotestosterone influenced numbers of healthy follicles and follicular amounts of LH receptor mRNA during the follicular phase of the estrous cycle in gilts

Reproduction ◽  
2008 ◽  
Vol 135 (3) ◽  
pp. 343-350 ◽  
Author(s):  
H Cárdenas ◽  
E Jiménez ◽  
W F Pope
Keyword(s):  
Estrous Cycle ◽  
Corn Oil ◽  
Follicular Phase ◽  
Lh Receptor ◽  
Receptor Mrna ◽  
Gonadotropin Receptor ◽  
Treatment Dose ◽  
Recruitment And Selection ◽  
Estradiol 17Β ◽  
Receptor Inhibitor

The present experiments were conducted to determine androgenic effects on numbers, health, and amounts of gonadotropin receptor mRNA in late developing follicles of gilts. Gilts (n=5 per group) received daily injections of one of the following treatments on days 13–16 or days 13–18 of the estrous cycle: corn oil, 5α-dihydrotestosterone (DHT, 10 mg), flutamide (1.5 g, an androgen receptor inhibitor), DHT (10 mg) plus flutamide (1.5 g), testosterone (10 mg), and testosterone (10 mg) plus flutamide (1.5 g). Ovarian follicles ≥5 mm in diameter were evaluated on day 17 or 19, 24 h after receiving the last treatment dose. Follicles were classified as healthy (H), moderately atretic (MA), or very atretic (VA). Treatment with DHT increased (P<0.05) the numbers of H follicles relative to control gilts on days 17 and 19. DHT administration from days 13 to 16 diminished (P<0.05) the amounts of LH receptor (LHR) mRNA in H follicles from day 17 (relative amounts: 1.45±0.33 and 2.72±0.33 for DHT- and vehicle-treated gilts respectively). The effects of DHT on numbers of H follicles and LHR mRNA were not observed in gilts receiving DHT plus flutamide. Androgens did not influence numbers of MA, VA, and total follicles, or follicular estradiol-17β concentrations and amounts of FSHR mRNA. Treating gilts with DHT during follicular recruitment and selection did not induce changes in the numbers of total follicles ≥5 mm, but rather increased the numbers of healthy follicles in this follicular population in association with decreased amounts of LHR mRNA.

scholarly journals Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

2005 ◽  
Vol 152 (3) ◽  
pp. 395-401 ◽  
Author(s):  
Femke P Hohmann ◽  
Joop S E Laven ◽  
Frank H de Jong ◽  
Bart C J M Fauser
Keyword(s):  
Ovarian Stimulation ◽  
Follicular Phase ◽  
Inhibin B ◽  
High Dose ◽  
Low Doses ◽  
Serum Concentrations ◽  
Inhibin A ◽  
Phase Group ◽  
The Relationship ◽  
Late Follicular Phase

Objective: To investigate the relationship between serum concentrations of inhibin A, inhibin B and estradiol (E2) and the number of developing follicles during the administration of exogenous follicle-stimulating hormone (FSH) in various regimens in normo-ovulatory volunteers and to evaluate if inhibins act as suitable markers for the number of developing follicles during ovarian stimulation. Design and methods: Serial hormone determinations and assessment of follicle numbers were carried out during unstimulated cycles and during various interventions with exogenous FSH. Subjects were randomized for FSH administration into the following groups: a single high dose (375 IU) during the early follicular phase (group A), 5 consecutive low doses (75 IU/day) starting in the mid follicular phase (group B) or daily low doses (75 IU/day) during the early to late follicular phase (starting on cycle days 3, 5 or 7; groups C, D and E respectively). Results: Extending the FSH window increases the number of small antral follicles and hence inhibin B serum concentrations. If such an intervention results in multi-follicular growth, mid follicular phase inhibin B (P = 0.001) as well as late follicular phase inhibin B and inhibin A levels are significantly (P < 0.05 and P < 0.01 respectively) increased compared with mono-follicular cycles or the natural cycle. Although mid follicular inhibin B levels correlated well with the number of small antral (P < 0.05) and pre-ovulatory (P < 0.001) follicles in the late follicular phase, mid follicular inhibin A and estradiol serum concentrations only correlated with the number of pre-ovulatory follicles (P < 0.001 and P < 0.01 respectively). Conclusions: The present data extend our understanding of the relationship between follicle dynamics, serum inhibins and FSH during ovarian hyperstimulation. However, although mid follicular inhibin B does correlate with the number of developing follicles, it does not facilitate the identification of women at risk for multiple follicle development.

Changes in Serum Concentrations of Prolactin, Progestagens, and Estradiol-17β and Biochemical Parameters During Peripartum in an Agalactic Mare

2013 ◽  
Vol 33 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Kenji Korosue ◽  
Harutaka Murase ◽  
Fumio Sato ◽  
Mutsuki Ishimaru ◽  
Gen Watanabe ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Serum Concentrations ◽  
Estradiol 17Β

A Simplified Radioimmunoassay for Human Pituitary Luteinizing Hormone in Serum

1971 ◽  
Vol 17 (9) ◽  
pp. 931-935 ◽  
Author(s):  
Robert A Levine ◽  
Richard K Donabedian ◽  
Luis G Sobrinho
Keyword(s):  
Luteinizing Hormone ◽  
Follicular Phase ◽  
Serum Concentrations ◽  
Human Pituitary ◽  
Clinical Diagnoses ◽  
Normal Males ◽  
Primary Gonadal Failure ◽  
Gonadal Failure

Abstract A simplified radioimmunoassay is described for human pituitary luteinizing hormone (LH). The principal modification over existing methods is that talc is used to separate antibody-bound hormone from free hormone by adsorption of the latter. Serum concentrations of LH in normal males, ovulatory females (follicular phase), postmenopausal females, patients with primary gonadal failure, and patients with hypopituitarism averaged 4.7, 4.6, 26.4, 30.8, and 1.9 µg of reference std. LER 907 per 100 ml of serum, respectively (10.3, 10.1, 57.8, 67.5, 4.2 milli-int. units/ml, respectively). These values are consistent with the clinical diagnoses.

Endocrinology of nutritional flushing in gilts

1991 ◽  
Vol 71 (4) ◽  
pp. 1063-1071 ◽  
Author(s):  
E. Beltranena ◽  
G. R. Foxcroft ◽  
F. X. Aherne ◽  
R. N. Kirkwood
Keyword(s):  
Plasma Insulin ◽  
Follicle Stimulating Hormone ◽  
Follicular Phase ◽  
Ovulation Rate ◽  
Feeding Level ◽  
Factor I ◽  
Igf I ◽  
Endocrine Status ◽  
Ad Libitum ◽  
Estradiol 17Β

As part of a nutritional flushing study, the endocrine status of gilts was compared to gain further knowledge of the mechanism(s) that mediate the restoration of ovulation rate. From 45.3 kg BW to puberty, 42 gilts were fed 2.0 kg d−1 (L) or ad libitum (H). From puberty to second estrus, one-half of the L gilts had their feed allowance increased to 2.8 kg d−1 (LH). Gilts in the H group continued to be fed ad libitum (HH). Gilts in the LH and H groups showed a greater increase in ovulation rate between first and second estrus than LL gilts (P < 0.05). Based on sampling every 4 h through the peri-estrous period of 28 gilts, feeding level did not affect mean plasma follicle-stimulating hormone (FSH), luteinizing hormone (pLH), estradiol-17β or progesterone concentrations (P > 0.05). During the follicular phase, however, LH and HH gilts had not only a greater proportion of samples > 1 ng mL−1 but also showed an increased mean pLH daily sample variance compared with LL gilts (P < 0.05). The latter parameters were interpreted as indicators of an increased frequency of episodic pLH release. Furthermore, HH gilts had greater (P < 0.05) plasma insulin concentrations through the peri-estrous period and greater insulin-like growth factor I (IGF-I) concentrations during the pLH-surge period than LL gilts. These results confirm that increased plasma insulin may be an important metabolic cue, which in an independent or synergistic action with gonadotropins and IGF-I mediates the ovulatory response to flush-feeding. Key words: Gilt, flushing, ovulation, gonadotropins, insulin, IGF-I.

Immunohistochemical determination of estrogen receptor-α in vaginal and tumor tissues of healthy and TVT-affected bitches and their relation to serum concentrations of estradiol-17β and progesterone

2006 ◽  
Vol 66 (6-7) ◽  
pp. 1587-1592 ◽  
Author(s):  
Claudia Prado de Brito ◽  
Clair Motos de Oliveira ◽  
Fernando Augusto Soares ◽  
Marcelo Faustino ◽  
Cláudio Alvarenga de Oliveira
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Serum Concentrations ◽  
Tumor Tissues ◽  
Estradiol 17Β

scholarly journals Estradiol-17β Pharmacokinetics and Histological Assessment of the Ovaries and Uterine Horns following Intramuscular Administration of Estradiol Cypionate in Feral Cats

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1708
Author(s):  
Timothy H. Hyndman ◽  
Kelly L. Algar ◽  
Andrew P. Woodward ◽  
Flaminia Coiacetto ◽  
Jordan O. Hampton ◽  
...  
Keyword(s):  
Time Course ◽  
Mixed Effects ◽  
Felis Catus ◽  
Serum Concentrations ◽  
Feral Cats ◽  
Linear Mixed Effects ◽  
Artificial Induction ◽  
Long Acting ◽  
Estrous Behavior ◽  
Estradiol 17Β

The control of feral cats (Felis catus) in Australia is a key biological conservation issue. Male cats are more difficult to control than female cats. Collared and tagged female cats displaying estrous behavior have been considered as a way to lure male cats and reveal their locations. As female cats are seasonal breeders, artificial induction of estrous behavior following the administration of a long-acting estrogen could improve their use for this purpose. Estradiol cypionate was intramuscularly administered to nine entire non-pregnant female feral cats, of unknown estrous status, at 0.1, 0.3, or 0.5 mg/kg. Mean peak serum concentrations of estradiol-17β were 365 pg/mL (0.1 mg/kg), 1281 pg/mL (0.3 mg/kg), and 1447 pg/mL (0.5 mg/kg). The time-course of estradiol-17β concentrations after various doses of estradiol cypionate was assessed using non-compartmental and non-linear mixed-effects methods. At the highest-studied dose (0.5 mg/kg), the 50th percentile of estradiol-17β concentrations exceeded 0.1 ng/mL for 11.8 days, and 0.05 ng/mL for 14.6 days. The duration increased with increasing dose. No signs of toxicity were noticed in any cat during the study. This information will be useful to ongoing studies that are investigating ways to reduce the abundance of feral cats in Australia, especially adult male cats.

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