Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos

2020 ◽  
Vol 55 (11) ◽  
pp. 1619-1628
Author(s):  
Rakesh Kumar Majhi ◽  
Ashutosh Kumar ◽  
Sunil C. Giri ◽  
Chandan Goswami
Keyword(s):  
Differential Expression ◽  
Anas Platyrhynchos ◽  
Mature Sperm ◽  
Trpv Channels

Differential Expression Levels Assessment of Avian E2A Gene in Two Chinese Indigenous Duck Breeds (Anas platyrhynchos) by Semi-Quantitative RT-PCR

2006 ◽  
Vol 5 (9) ◽  
pp. 895-899 ◽  
Author(s):  
Dafaalla M. Mekki . ◽  
Bian Liang-yong . ◽  
Wang Jin-yu . ◽  
Yang Yan . ◽  
Li lin-chuan . ◽  
...  
Keyword(s):  
Differential Expression ◽  
Anas Platyrhynchos ◽  
Rt Pcr ◽  
Expression Levels

scholarly journals Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

2020 ◽  
Author(s):  
Rakesh Kumar Majhi ◽  
Ashutosh Kumar ◽  
Sunil C. Giri ◽  
Chandan Goswami
Keyword(s):  
Transient Receptor Potential ◽  
Anas Platyrhynchos ◽  
Avian Species ◽  
Confocal Imaging ◽  
Pekin Duck ◽  
Sperm Cells ◽  
Transient Receptor Potential Vanilloid ◽  
Poultry Production ◽  
Endogenous Expression ◽  
Trpv Channels

AbstractSperm cells have the ability of precise chemotactic and thermotactic movement which is crucial for fertilization, yet the key molecules involved in the detection of different chemical and physical stimuli and guide the sperm cells for proper navigation are not known. This aspect is more complex as each species have their own reproductive identity. Never-the-less, Ca2+-signaling and thus a series of Ca2+-channels seem to coordinate in order to regulate different functions mediated by sperm cells. However, such aspects are controlled by different Ca2+ channels and have species-specific differences. In this work we explored if TRPV channels are endogenously expressed in the mature spermatozoa obtained from avian species. We have used the sperm cells of white pekin duck (Anas platyrhynchos) as a representative avian species to explore the endogenous expression and localization of different TRPV channels. Western blot analysis (WB), flow cytometry, confocal imaging and super resolution imaging was performed for the characterization. Our results strongly suggest the expression and distinct localization of different TRPV channels in the sperm cells. All these TRPV channels are mainly absent in the head region. Only TRPV3 and TRPV4 are sparsely present in the neck region enriched with mitochondria. All these channels (TRPV1-6) are present in the tail region. The differential localization of TRPVs in duck sperm indicate their respective functions relevant in fertilization process of avian sperm. These findings may also have commercial importance in poultry production, cryopreservation of sperm as well as conservation of endangered species through artificial insemination.

Differential expression of ASS1 in serous and non-serous ovarian carcinomas

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Dong-Joo Cheon ◽  
Ann E. Walts ◽  
Jessica A. Beach ◽  
Jenny Lester ◽  
John S. Bomalaski ◽  
...  
Keyword(s):  
Differential Expression ◽  
Ovarian Carcinomas

Spermatogenesis in the Dragonfly with Particular Reference to the Cytoplasmic Organelles

1972 ◽  
Vol 30 ◽  
pp. 110-111
Author(s):  
Sant S. Sekhon
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Mature Sperm ◽  
Electron Microscopic ◽  
Cytoplasmic Organelles ◽  
Microscopic Studies ◽  
Insect Sperm ◽  
Large Round

Although there have been numerous studies concerning the morphogenetic changes accompanying the maturation of insect sperm, only a few deal with the sperm differentiation in the dragonflies. In two recent electron microscopic studies Kessel, has comprehensively treated the erlationship of microtubules to the nucleus and mid-piece structures during spermiogenesis in the dragonfly. The purpose of this study is to follow the sequential nuclear and cytoplasmic changes which accompany the differentiation of spermatogonium into a mature sperm during spermatogenesis in the dragonfly (Aeschna sp.).The dragonfly spermatogonia are characterized by large round nuclei. Loosely organized chromatin is usually unevenly distributed within the spermatogonial nuclei. The scant cytoplasm surrounding the nucleus contains mitochondria, the Golgi apparatus, elements of endoplasmic reticulum and numerous ribosomes (Fig. 1).

764: Differential Expression of RAL GTPases and their Effectors in Bladder Cancer

2007 ◽  
Vol 177 (4S) ◽  
pp. 256-256
Author(s):  
Steven Smith ◽  
Gary Oxford ◽  
Dan Theodorescu
Keyword(s):  
Bladder Cancer ◽  
Differential Expression ◽  
Ral Gtpases
Sign in / Sign up

Export Citation Format

Share Document