Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG

2020 ◽  
Vol 55 (3) ◽  
pp. 374-383
Author(s):  
Marta Dordas‐Perpinyà ◽  
Laure Normandin ◽  
Thibault Dhier ◽  
Hubert Terris ◽  
Anaïs Cochard ◽  
...  
Keyword(s):  
Saline Solution ◽  
Single Injection ◽  
Buserelin Acetate

The stress effects of a single injection of isotonic saline solution: systemic (blood) and central (frontal cortex and dorsal and ventral hippocampus)

2016 ◽  
Vol 10 (2) ◽  
pp. 115-119 ◽  
Author(s):  
S. V. Freiman ◽  
M. V. Onufriev ◽  
M. Yu. Stepanichev ◽  
Yu. V. Moiseeva ◽  
N. A. Lazareva ◽  
...  
Keyword(s):  
Frontal Cortex ◽  
Saline Solution ◽  
Single Injection ◽  
Isotonic Saline ◽  
Ventral Hippocampus ◽  
Systemic Blood ◽  
Stress Effects

scholarly journals Decreased astrocytic GFAP expression in streptozotocin-induced diabetes after gliotoxic lesion in the rat brainstem

2013 ◽  
Vol 57 (6) ◽  
pp. 431-436 ◽  
Author(s):  
Eduardo Fernandes Bondan ◽  
Maria de Fátima Monteiro Martins ◽  
Flávio Cesar Viani
Keyword(s):  
Saline Solution ◽  
Single Injection ◽  
Diabetic Rats ◽  
Male Rats ◽  
Image Analysis System ◽  
Immunohistochemical Expression ◽  
Gfap Expression ◽  
Streptozotocin Induced Diabetes ◽  
Rat Brainstem ◽  
Analysis System

OBJECTIVE: The aim of this study was to evaluate the effect of diabetic hyperglycemia on astrocyte function, estimated by means of glial fibrillary acidic protein - GFAP - immunohistochemical expression. MATERIALS AND METHODS: Adult male rats received a single intravenous injection of streptozotocin (50 mg/kg) and were submitted 10 days later to a single injection of 10 microlitres 0.1% EB solution or 0.9% saline solution into the cisterna pontis. Ten microliters of 0.1% EB or 0.9% saline solution were also injected in non-diabetic rats. Animals were anesthetized and perfused through the heart 15 and 31 days after EB or saline injection, and brainstem sections were collected for ultrastructural analysis and GFAP immunohistochemical staining. RESULTS: The GFAP brown-stained areas were evaluated by colorimetry using a computerized image analysis system and the results have shown that diabetes hindered the increase of GFAP astrocyte expression in the EB-injected group compared to non-diabetic animals. However, diabetes did not affect GFAP response in the saline-injected group or in control animals. CONCLUSION: Streptozotocin-induced diabetic condition reduced astrocytic GFAP expression following gliotoxic injury.

165 EFFECT OF INJECTION OF SEMINAL PLASMA ON OVULATION RATE, CORPUS LUTEUM DEVELOPMENT, AND SENSITIVITY TO PROSTAGLANDIN IN ALPACAS (VICUGNA PACOS)

2015 ◽  
Vol 27 (1) ◽  
pp. 173
Author(s):  
W. F. Huanca ◽  
C. Mamani ◽  
W. Huanca
Keyword(s):  
Corpus Luteum ◽  
Seminal Plasma ◽  
Saline Solution ◽  
Ovulation Rate ◽  
Similar Response ◽  
Linear Transducer ◽  
Vicugna Pacos ◽  
Buserelin Acetate ◽  
T1 And T2 ◽  
Control Application

The seminal plasma (SP) of camelids contains a protein identified as β Nerve Growth Factor with capacity of induced ovulation and develop a corpus luteum. A study was designed to evaluate the effect of application of SP on the interval of time from injection of stimulus to the ovulation and corpus luteum (CL) size (Experiment 1, n = 24) and on the sensitivity of CL to the injection of prostaglandin (196 µg of tiaprost) (PG) at different periods from ovulation (Experiment 2, n = 86). Exp. 1: Adult female alpacas with presence of a follicle ≥7 mm were assigned to the application of 1 mL of SP via IM (T: n = 12) or application of 50 µg of acetate of busereline IM (T2: n = 12). Exp. 2: Alpacas with presence of a follicle ≥7 mm were induced to ovulation with 50 µg of acetate of busereline or 1 mL IM of SP. Animals were evaluated by ultrasound to confirm the ovulation and were assigned to the following treatment: T1 (n = 8): SP + PG Day 4; T2 (n = 8): buserelin acetate + PG Day 4; T3 (n = 8): SP + PG Day 5; T4 (n = 8): buserelin acetate + PG Day 5; T5 (n = 8): SP + PG Day 6; T6 (n = 8): buserelin acetate + PG Day 6; T7 (n = 8): SP + PG Day 7; T8 (n = 8): buserelin acetate + PG Day 7; T9 (n = 8): SP + PG Day 8; T10 (n = 8): buserelin acetate + PG Day 8 and T11 (n = 6) (Control): Application of 1 mL of saline solution. The animals were evaluated by ultrasound with an Aloka SSD500 (Aloka, Tokyo, Japan) and 7.5-MHz linear transducer each 2 h (Exp. 1) and each 12 h (Exp. 2) after of application of PG. In Exp. 1, the ovulation rate was 95.7% to T1 and T2 and an interval of time between injection of stimulus and ovulation was 27.4 ± 2.5 h and 26.8 ± 1.8 to T1 and T2, respectively. In Exp. 2, luteolysis was 0.0%, 0.0%, 25.0%, 0.0%, 100.0%, 100.0%, 100.0%, 100.0%, 100.0%, 100.0%, and 0.0% for the treatments T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, and T11 respectively. The results suggest that no differences exist between in the ovulation rate and interval to the ovulation between the application of buserelin acetate or SP and that the CL was sensible at Day 5 to the prostaglandin respect SP and with similar response to the sensibility of CL from Day 6 to Day 8.

STUDIES ON THE INACTIVATION OF PANCREOZYMIN CONCENTRATES

1970 ◽  
Vol 63 (3) ◽  
pp. 519-526 ◽  
Author(s):  
Kaarlo Hartiala ◽  
Leon L. Gershbein
Keyword(s):  
Main Pancreatic Duct ◽  
Diamine Oxidase ◽  
Saline Solution ◽  
Metal Salt ◽  
Liver Extract ◽  
Single Injection ◽  
Fluid Secretion ◽  
Canine Plasma ◽  
Heavy Metal Salt ◽  
Hog Kidney

ABSTRACT The loss in hormonal activity resulting on incubation of pancreozymin concentrates with canine blood serum at 37°C has been investigated. The test extracts comprised (SI), a solid obtained from treatment of a salted acid extract of the upper intestinal mucosa with trichloroacetic acid and the amine precipitate, (AP), derived from SI. The decrease in activity was determined in relation to an incubated concentrate-saline solution in dogs with the main pancreatic duct cannulated. Fluid secretion was promoted by potent secretin preparations in saline administered by single injection or by continuous infusion. As based on the total amylase content of the pancreatic juice samples, the hormonal concentrates underwent rather small losses in activity, the AP requiring higher dilutions with serum for an effect as compared to SI. Canine liver extract and a hog kidney diamine oxidase concentrate caused inactivation of pancreozymin products but bovine and canine plasma protein fractions, heavy metal salt and thiol solutions and possibly, human urine, were without action.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

Quantifying in vivo dopaminergic D2-receptors Bmax and KdVr with microPET® focus after a single injection of [11C]raclopride

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S649-S649
Author(s):  
Laurent Besret ◽  
Jean-Dominique Gallezot ◽  
Frédéric Dollé ◽  
Philippe Hantraye ◽  
Marie-Claude Grégoire
Keyword(s):  
Single Injection ◽  
D2 Receptors ◽  
Quantifying In
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