Effects of recipient oocyte source, number of transferred embryos and season on somatic cell nuclear transfer efficiency in sheep

2019 ◽  
Vol 54 (11) ◽  
pp. 1443-1448 ◽  
Author(s):  
Yixin Yuan ◽  
Ruming Liu ◽  
Xiaosheng Zhang ◽  
Jinlong Zhang ◽  
Zi Zheng ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Transfer Efficiency ◽  
Source Number

Developmental potential of pig embryos reconstructed by use of sow versus pre-pubertal gilt oocytes after somatic cell nuclear transfer

Zygote ◽  
2013 ◽  
Vol 22 (3) ◽  
pp. 356-365 ◽  
Author(s):  
Juan Li ◽  
Hanne Skovsgaard Pedersen ◽  
Rong Li ◽  
Janne Adamsen ◽  
Ying Liu ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Follicular Fluid ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Control Experiment ◽  
Transfer Efficiency ◽  
Maturation Time ◽  
Developmental Potential ◽  
Routine Production ◽  
Cloning Experiment

SummaryIn this study, the developmental ability of cloned embryos using gilt versus sow oocytes was evaluated under the hypothesis that the efficiency of nuclear transfer using gilt oocytes was lower than that of sow oocytes, but that it could be optimized. Five experiments were performed with routine production of cloned embryos with sow oocytes serving as the control. Results showed that: Experiment 1: Blastocyst rates of cloned embryos with gilt oocytes was about half compared with control. Experiment 2: An extended maturation time of 48 h used for gilt oocytes resulted in lower blastocyst rates after cloning. Experiment 3: Development of cloned embryos with gilt oocytes was improved by co-culture with sow oocytes. Experiment 4: After maturation of gilt oocytes using follicular fluid from gilt instead of sow, the oocytes were sorted into large and small oocytes, and after cloning, blastocyst rates were higher using large gilt oocytes compared with small oocytes; however, the rate remained lower compared with control. Experiment 5: Six sow recipients received a total of 503 morulae and blastocysts cloned from gilt oocytes (four recipients) and 190 cloned from sow oocytes (two recipients). All recipients became pregnant and went to term, resulting in 26 (gilt oocytes) and six (sow oocytes) piglets. In conclusion, results confirmed that nuclear transfer efficiency was higher using sow versus gilt oocytes, but the use of gilt oocytes can be optimized by sorting after ooplasm size following maturation and by maturing gilt and sow oocytes together.

Effect of oocyte mitochondrial DNA haplotype on bovine somatic cell nuclear transfer efficiency

2007 ◽  
Vol 74 (10) ◽  
pp. 1278-1286 ◽  
Author(s):  
Fei Jiao ◽  
Jing-Bin Yan ◽  
Xiao-Yu Yang ◽  
Hua Li ◽  
Qingxue Wang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Transfer Efficiency ◽  
Mitochondrial Dna Haplotype

scholarly journals Factors influencing the somatic cell nuclear transfer efficiency in pigs

2019 ◽  
Vol 6 (1) ◽  
pp. 73 ◽  
Author(s):  
Yong JIN ◽  
Manling ZHANG ◽  
Xinrong JU ◽  
Shuang LIANG ◽  
Qiang XIONG ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Transfer Efficiency ◽  
Factors Influencing

scholarly journals Overexpression of Tet3 in donor cells enhances goat somatic cell nuclear transfer efficiency

FEBS Journal ◽  
2018 ◽  
Vol 285 (14) ◽  
pp. 2708-2723 ◽  
Author(s):  
Chengquan Han ◽  
Ruizhi Deng ◽  
Tingchao Mao ◽  
Yan Luo ◽  
Biao Wei ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Transfer Efficiency ◽  
Donor Cells
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