scholarly journals Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm-Ovis-Halomax during in vitro capacitation of cryopreserved ram spermatozoa

2019 ◽  
Vol 54 ◽  
pp. 46-49 ◽  
Author(s):  
Patricia Peris-Frau ◽  
Manuel Álvarez-Rodríguez ◽  
Alicia Martín-Maestro ◽  
María Iniesta-Cuerda ◽  
Irene Sánchez-Ajofrín ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Comparative Evaluation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Chromatin Structure Assay

scholarly journals Sperm chromatin structure assay versus sperm chromatin dispersion kits: Technical repeatability and choice of assisted reproductive technology procedure

2020 ◽  
Vol 47 (4) ◽  
pp. 277-283
Author(s):  
Vidya Laxme B ◽  
Silviya Stephen ◽  
Ramyashree Devaraj ◽  
Sridurga Mithraprabhu ◽  
Ricardo P. Bertolla ◽  
...  
Keyword(s):  
Assisted Reproductive Technology ◽  
Chromatin Structure ◽  
Reproductive Technology ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Vitro Fertilization ◽  
Sperm Chromatin Structure Assay ◽  
Sperm Dna ◽  
The Impact

Objective: The sperm DNA fragmentation index (DFI) guides the clinician’s choice of an appropriate assisted reproductive technology (ART) procedure. The DFI can be determined using commercially available methodologies, including sperm chromatin dispersion (SCD) kits and sperm chromatin structure assay (SCSA). Currently, when DFI is evaluated using SCD kits, the result is analyzed in reference to the SCSA-derived threshold for the choice of an ART procedure. In this study, we compared DFI values obtained using SCSA with those obtained using SCD and determined whether the difference affects the choice of ART procedure.Methods: We compared SCSA to two SCD kits, CANfrag (n=36) and Halosperm (n=31), to assess the DFI values obtained, the correlations between tests, the technical repeatability, and the impact of DFI on the choice of ART. Results: We obtained higher median DFI values using SCD kits than when using SCSA, and this difference was significant for the CANfrag kit (p<0.001). The SCD kits had significantly higher coefficients of variation than SCSA (p<0.0001). In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) would be chosen for a significantly higher proportion of patients if a decision were made based on DFI derived from SCD rather than DFI determined using SCSA (p=0.003). Conclusion: Our results indicate that SCD kit-specific thresholds should be established in order to avoid the unnecessary use of IVF/ICSI based on sperm DNA damage for the management of infertility. Appropriate measures should be taken to mitigate the increased variability inherent to the methods used in these tests.

Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects

1999 ◽  
Vol 11 (1) ◽  
pp. 1 ◽  
Author(s):  
D. P. Evenson
Keyword(s):  
Chromatin Structure ◽  
Male Fertility ◽  
Nuclear Dna ◽  
Early Embryo ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fertility Potential ◽  
Post Stress

An important goal of modern analyses of semen is to elucidate the molecular traits of mammalian sperm chromatin structural abnormalities, defined here as ‘uncompensable’, that lead to abnormalities in fertility, pronuclear formation, early embryo quality and pregnancy outcome. Sperm with uncompensable nuclear abnormalities are able to fertilize oocytes both in vivo and in vitro; however, due to the uncompensable trait(s), the embryo development may be abnormal. Uncompensable nuclear traits can be experimentally induced in bull sperm by a mild thermal insult to the testis. Sperm nuclear morphology abnormalities seen in ejaculates 11-days post stress are likely related to molecular changes in chromatin observed 3-days post stress by the flow cytometric sperm chromatin structure assay (SCSA). The SCSA measures the susceptibility of sperm nuclear DNA to denaturation in situ. This susceptibility has been correlated with the presence of DNA strand breaks that may be derived in part by oxidative stress and possibly by a unique, abortive apoptotic mechanism. The extent of DNA denaturation is not significantly related to the level of disulfide bonding between the chromatin protamines. The use of human sperm with uncompensable nuclear traits for artificial reproductive techniques is also discussed. The goal of this research is to remove from semen doses those sperm with uncompensable nuclear traits and thereby increase male fertility potential. Extra key words: male fertility potential, sperm chromatin structure assay (SCSA).

DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay

2005 ◽  
Vol 63 (6) ◽  
pp. 1789-1802 ◽  
Author(s):  
Gry B. Boe-Hansen ◽  
Ian D. Morris ◽  
Annette K. Ersbøll ◽  
Torben Greve ◽  
Preben Christensen
Keyword(s):  
Comet Assay ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Chromatin Structure Assay ◽  
Neutral Comet Assay ◽  
Bull Sperm
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