Comparison of the intravenous and intravaginal route of oxytocin administration for cervical dilation protocol and non‐surgical embryo recovery in oestrous‐induced Santa Inês ewes

2019 ◽  
Vol 54 (9) ◽  
pp. 1230-1235 ◽  
Author(s):  
Lucia Prellwitz ◽  
Fabiana Nunes Zambrini ◽  
José Domingos Guimarães ◽  
Marco Antonio Paula de Sousa ◽  
Maria Emília Franco Oliveira ◽  
...  
Keyword(s):  
Cervical Dilation ◽  
Embryo Recovery ◽  
Santa Inês

Effect of season and frequency of embryo collections on superovulatory response and embryo recovery in Santa Inês hair sheep

2021 ◽  
pp. 106441
Author(s):  
Monique de Albuquerque Lagares ◽  
Fabiana Cristina Varago ◽  
Valéria Spyridion Moustacas ◽  
Valentim Arabicano Gheller ◽  
Rafael Romero Nicolino ◽  
...  
Keyword(s):  
Hair Sheep ◽  
Embryo Recovery ◽  
Santa Inês

227 Is the antral follicle count on a random day of the oestrous cycle correlated with superovulatory responses in Santa Inês ewes?

2020 ◽  
Vol 32 (2) ◽  
pp. 241
Author(s):  
M. Pupin ◽  
G. Vergani ◽  
M. Lima ◽  
K. Silva ◽  
A. Monteiro ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Animal Health ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Flunixin Meglumine ◽  
Santa Inês ◽  
Synchronization Protocol

Antral follicle count (AFC) performed after an oestrus synchronization protocol has been studied as a tool to select ewes with high potential for invivo embryo production (Pinto et al. 2018 Theriogenology 113, 146-152). However, it would be interesting to know whether AFC assessed on a random day of the oestrous cycle correlates with the superovulatory response. The present study was conducted to evaluate the correlation between AFC at the beginning of progesterone (P4)-based oestrus synchronization protocol used as basis of superovulatory treatment and the number of corpora lutea (CL) 12h before recovery of embryos in Santa Inês ewes. The study was conducted during September and October in northeast Brazil (03°40′26″S and 40°14′20″W) using 8 adult Santa Inês ewes. On a random day of oestrous cycle (Day 0) all ewes received an intravaginal device (CIDR) of progesterone (0.3g, Eazi-breed, Zoetis), which remained for 9 days. On Day 7, the pFSH (133mg, Folltropin V, Vetoquinol) treatment began, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) injected IM at 12-h intervals. On Day 9, 2 equal doses of D-cloprostenol were injected at a 12-h interval (37.5µg, Prolise, Agener União). All ewes showed oestrus and were mated by fertile rams. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was administered IM on Days 12, 13, and 15. On Day 16, non-surgical embryo recovery (NSER) was performed after cervical dilation using D-cloprostenol and oestradiol benzoate at 16h and oxytocin 20min before. Transrectal B-mode ultrasound evaluations (Z5 Vet, Mindray), frequency 7.5MHz, were performed on Day 0 and 7 and 12h before NSER to evaluate the ovarian population present. Pearson correlation analysis (P<0.05) was performed using Bioestat 5.3 software. The number of AFC per ewe at the beginning of the protocol and on Day 7 were 9.9±2.7 and 11±3.2, respectively. The numbers of CL, recovered embryos, and viable embryos were 14.0±3.5, 8.2±10.9, and 6.0±11.0, respectively. There was no correlation of AFC on a random day of oestrous cycle with the number of AFC on Day 7 (P=0.42), number of corpora lutea (P=0.44), number of recovered embryos (P=0.18), or number of viable embryos (P=0.11) in superovulated ewes. In conclusion, we did not find significant correlations between AFC on a random day of oestrous cycle and the superovulatory/embryos response in Santa Inês ewes. Financial support for this study was provided by Embrapa (02.13.06.026.00.02 and 02.13.06.026.00.04) and FAPEMIG (PPM 00201-17).

Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH

2003 ◽  
Vol 49 (1) ◽  
pp. 19-23 ◽  
Author(s):  
M.F. Cordeiro ◽  
J.B. Lima-Verde ◽  
E.S. Lopes-Júnior ◽  
D.I.A. Teixeira ◽  
L.N. Farias ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Embryo Recovery ◽  
Santa Inês

225 Evaluation of superovulatory response in Brazilian native sheep by B-mode ultrasonography

2020 ◽  
Vol 32 (2) ◽  
pp. 240
Author(s):  
G. B. Vergani ◽  
M. S. D. Lima ◽  
K. M. Silva ◽  
A. W. U. Monteiro ◽  
A. F. Ramos ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Pearson Correlation ◽  
Animal Health ◽  
Device Removal ◽  
Embryo Recovery ◽  
Flunixin Meglumine ◽  
Ultrasound Evaluation ◽  
Release Device ◽  
Linear Probe ◽  
Santa Inês

The objective of this study was to evaluate the correlation between the number of corpora lutea (CL), as determined by B-mode ultrasonography, and the total number of structures recovery by non-surgical embryo recovery (NSER) from superovulated ewes. Somali (n=18), Santa Inês (n=18), and Morada Nova (n=16) ewes received an intravaginal device with progesterone (0.33g, controlled internal drug release device, CIDR, Zoetis), which was maintained for 9 days. The superovulatory protocol started 60h before device removal, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) of p-FSH (IM 133mg, Folltropin V, Vetoquinol) injected every 12h. D-Cloprostenol (IM 37.5μg, Prolise, Agener Union) was injected 12h before and at the moment of device removal. Ewes in oestrus were mated by fertile male rams three times after the onset of oestrus with a 12-h interval. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was injected IM on Days 12, 13, and 15. One day before NSER, B-mode ultrasound evaluations were performed using portable equipment (Z5 Vet, Mindray) with a stiffened multifrequency linear probe to evaluate the number of structures present in the ovaries. Embryo recovery was performed 7 days after progesterone CIDR removal (Day 16). The number of CL and the number of recovered structures were determined and their association evaluated using the Pearson correlation test (P<0.05). Only 17 Somali, 16 Santa Inês, and 15 Morada Nova ewes were submitted to NSER; the other 4 ewes could not be flushed because of low cervical dilation or cervical puncture. After B-mode ultrasound evaluation, 168, 217, and 131 CL were observed, with an average of 9.9 (range: 1 to 12), 13.5 (range: 2 to 47), and 8.7 (range: 3 to 18) CL per donor for Somali, Santa Inês, and Morada Nova ewes, respectively. The recovery rates (i.e. structures recovered by the number of CL counted) were 60.1% (101/168), 96.3% (209/217), and 103.5% (135/131) for the 3 breeds, respectively. There was a positive correlation (r=0.69; P<0.01) between the number of recovered structures and number of CL. Those findings confirm results from other studies, in which the recovery rate varied from 35 to 91%. In some cases, recovery rate can be overestimated because of the difficulty in quantifying CL. In conclusion, B-mode ultrasonography can be used to estimate superovulatory response in ewes and thus as a criterion to decide whether a donor will undergo embryo flushing. Financial support for this study was provided by Embrapa (02.13.06.026.00.04) and Fapemig (CVZ-PPM 00201-17).

226 Effect of the presence or absence of corpus luteum in females with small antral follicles at the beginning of a progesterone-based oestrus synchronization protocol on superovulatory response and embryo yields in sheep

2020 ◽  
Vol 32 (2) ◽  
pp. 241
Author(s):  
M. S. D. Lima ◽  
G. B. Vergani ◽  
K. M. Silva ◽  
A. W. U. Monteiro ◽  
L. M. C. Pereira ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Animal Health ◽  
Cervical Dilation ◽  
Antral Follicles ◽  
Embryo Recovery ◽  
Multiple Ovulation ◽  
Flunixin Meglumine ◽  
Oestradiol Benzoate ◽  
Intravaginal Device ◽  
Synchronization Protocol

The high variability of responses to multiple ovulation and embryo transfer (MOET) programs is related to several factors, including the ovarian population present at the beginning of hormonal protocols. Thus, our objective was to evaluate the effect of the presence or absence of corpus luteum (CL) in females presenting small antral follicles (SF) at the beginning of a progesterone (P4)-based oestrus synchronization protocol on superovulatory response and embryo yields. The study was conducted during September and October in northeast Brazil (03°40′26″S and 40°14′20″W) using 15 adult Santa Inês ewes, in which the ovarian population present were SF (≤3.75mm, n=5) or SF plus CL (SF+CL, n=10) at the beginning of P4-based oestrus synchronization protocol. The females received a new or reused intravaginal device of progesterone (CIDR Eazi-breed, Zoetis) on Day 0, which remained for 9 days. On Day 7, the pFSH (133mg, Folltropin V, Vetoquinol) treatment was begun, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) injected IM at 12-h intervals. On Day 9, two equal doses of D-cloprostenol were injected at a 12-h interval (37.5μg, Prolise, Agener União). Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was administered IM on Days 13 to 15. All ewes showed oestrus and were mated with fertile rams. On Day 16 non-surgical embryo recovery (NSER) was performed after cervical dilation using D-cloprostenol and oestradiol benzoate at 16h and ocytocin at 20min before. Transrectal B-mode ultrasound evaluations (Z5 Vet, Mindray) were performed on Day 0 and just before NSER to evaluate the ovarian population present. Data are showed in mean±s.d. and compared by t-test for independent samples (P<0.05). The number of CL present on NSER day (21.5±4.4 and 4.0±8.5) and the number of viable embryos (11.1±14.9 and 4.0±8.5) differed (P<0.05) between SF+CL ewes and SF ewes, respectively. However, the number of luteinizing unovulated follicles present on NSER day (4.5±2.5 and 12.4±7.8) and the number of recovered structures (i.e. embryos plus unfertilized eggs; 14.2±15.6 and 4.2±8.2) did not differ (P>0.05) between SF+CL ewes and SF ewes, respectively. In conclusion, the presence of a CL at the beginning of the oestrous synchronization protocol used for superovulation treatment affects responses to the MOET program. Financial support for this study was provided by Embrapa (02.13.06.026.00.02 and 02.13.06.026.00.04) and FAPEMIG (PPM 00201-17).

Successive in vivo embryo production in Santa Inês sheep

2020 ◽  
Vol 60 (4) ◽  
pp. 497 ◽  
Author(s):  
Pedro Henrique Nicolau Pinto ◽  
Mario Felipe Alvarez Balaro ◽  
Helena Fabiana Reis de Almeida Saraiva ◽  
Viviane Lopes Brair ◽  
Vivian Angélico Pereira Alfradique ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Doppler Ultrasonography ◽  
Corpora Lutea ◽  
Colour Doppler ◽  
Embryo Production ◽  
Previous Response ◽  
Embryo Recovery ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P < 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P < 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P < 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, >10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

Combined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

2018 ◽  
Vol 54 (1) ◽  
pp. 118-125 ◽  
Author(s):  
Jeferson Ferreira da Fonseca ◽  
Fabiana Nunes Zambrini ◽  
José Domingos Guimarães ◽  
Marcio Roberto Silva ◽  
Maria Emilia Franco Oliveira ◽  
...  
Keyword(s):  
Combined Treatment ◽  
Cervical Dilation ◽  
Embryo Recovery ◽  
Oestradiol Benzoate

scholarly journals Cervical relaxation for non-surgical uterus access in Santa Inês ewes

2018 ◽  
Vol 70 (6) ◽  
pp. 1671-1679 ◽  
Author(s):  
C.R. Leite ◽  
J.F. Fonseca ◽  
D.A.M. Fernandes ◽  
J.M.G. Souza-Fabjan ◽  
F.O. Ascoli ◽  
...  
Keyword(s):  
Epidural Anesthesia ◽  
Saline Solution ◽  
Control Treatment ◽  
Cervical Dilation ◽  
Surgical Access ◽  
Transposition Rate ◽  
Santa Inês ◽  
Experimental Treatments ◽  
Hormonal Treatments

ABSTRACT The present study was composed by two experiments aiming to develop a cervical dilation technique for non-surgical access to Santa Inês ewe’s uterus. In Experiment 1, thirty ewes underwent four epidural treatments. The three experimental treatments used 2.0mg/kg ketamine. The group receiving this drug alone was denominated KG, whereas other group had ketamine associated with 0.1mg/kg morphine (KM) and KX a third group had ketamine associated with 0.05mg/kg xylazine (KX). Control treatment was 1mL/7.5kg saline solution epidurally (CON). Cervical dilation was evaluated in both experiments by attempting to pass a metal rod through the cervix. In Experiment 2, three different hormonal protocols for cervical dilation were tested in thirty ewes. Epidural anesthesia with 2.0mg/kg ketamine was the control treatment (KG) and was combined with hormonal treatments: Misoprostol (MI); Oxytocin + Estradiol (OE); Misoprostol + Oxytocin + Estradiol (MOE). In Experiment 1 transposition rate was not different among groups. In Experiment 2, OE presented the highest rate (90%) while MOE presented 86.2%, MI 68.9% and CON 62.1%. The study developed a pharmacological protocol that increased cervical transposition making the non-surgical access to the uterus feasible in Santa Inês ewes.

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