Method agreement between three different chambers for comparative boar semen CASA analysis

2019 ◽  
Author(s):  
A. Basioura ◽  
G. Tsousis ◽  
C. Boscos ◽  
A. Lymberopoulos ◽  
I. Tsakmakidis
Keyword(s):  
Boar Semen ◽  
Method Agreement

Fertilization Rates Obtained with Boar Semen Stored for Zero and Twelve Hours

1958 ◽  
Vol 17 (3) ◽  
pp. 598-603 ◽  
Author(s):  
Frederick W. Stratman ◽  
H. L. Self ◽  
Vearl R. Smith
Keyword(s):  
Fertilization Rates ◽  
Boar Semen

scholarly journals Cluster analysis reveals seasonal variation of sperm subpopulations in extended boar semen

2018 ◽  
Vol 64 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Iulian IBĂNESCU ◽  
Claus LEIDING ◽  
Heinrich BOLLWEIN
Keyword(s):  
Cluster Analysis ◽  
Seasonal Variation ◽  
Boar Semen

Fertility with antibiotic-free preserved boar semen under standardized field conditions

2020 ◽  
Vol 220 ◽  
pp. 106410
Author(s):  
Helen Jäkel ◽  
Rafael Gianluppi ◽  
Matheus Schardong Lucca ◽  
Ana Paula Mellagi ◽  
Rafael Rosa Ulguim ◽  
...  
Keyword(s):  
Field Conditions ◽  
Boar Semen

scholarly journals Thawing boar semen in the presence of seminal plasma improves motility, modifies subpopulation patterns and reduces chromatin alterations

2017 ◽  
Vol 29 (8) ◽  
pp. 1576 ◽  
Author(s):  
Rocío Fernández-Gago ◽  
Manuel Álvarez-Rodríguez ◽  
Marta E. Alonso ◽  
J. Ramiro González ◽  
Beatriz Alegre ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Chromatin Compaction ◽  
Sperm Analysis ◽  
Positive Effects ◽  
Fragmentation Index ◽  
Non Linear ◽  
Boar Semen ◽  
Dna Fragmentation Index

Seminal plasma could have positive effects on boar semen after thawing. In the present study we investigated changes in the motility and chromatin structure in spermatozoa over 4 h incubation (37°C) of boar semen thawed in the presence of 0%, 10% or 50% seminal plasma from good-fertility boars. Cryopreserved doses were used from seven males, three of which were identified as susceptible to post-thawing chromatin alterations. Motility was analysed by computer-aided sperm analysis every hour, and data were used in a two-step clustering, yielding three subpopulations of spermatozoa (slow non-linear, fast non-linear, fast linear). Chromatin structure was analysed using a sperm chromatin structure assay and flow cytometry to determine the DNA fragmentation index (%DFI) as a percentage, the standard deviation of the DFI (SD-DFI) and the percentage of high DNA stainability (%HDS), indicating chromatin compaction. Thawing without seminal plasma resulted in a rapid loss of motility, whereas seminal plasma helped maintain motility throughout the incubation period and preserved the subpopulation comprising fast and linear spermatozoa. The incidence of chromatin alterations was very low in samples from non-susceptible males, whereas samples from males susceptible to post-thawing chromatin alterations exhibited marked alterations in %DFI and %HDS. Seminal plasma partly prevented these alterations in samples from susceptible males. Overall, 50% seminal plasma was the most efficient concentration to protect motility and chromatin. Some changes were concomitant with physiological events reported previously (e.g., semen thawed with 50% seminal plasma increased the production of reactive oxygen species and yielded higher fertility after AI). Thawing in the presence of seminal plasma could be particularly useful in the case of samples susceptible to post-thawing chromatin damage.

Factors influencing the response of spermatozoa to agitation stress: Implications for transport of extended boar semen

2021 ◽  
Author(s):  
Aline FL. Paschoal ◽  
Anne-Marie Luther ◽  
Ulrike Jakop ◽  
Martin Schulze ◽  
Fernando P. Bortolozzo ◽  
...  
Keyword(s):  
Factors Influencing ◽  
Boar Semen

Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks

2013 ◽  
Vol 84 (8) ◽  
pp. 600-606 ◽  
Author(s):  
Zhao Namula ◽  
Yoko Sato ◽  
Risa Kodama ◽  
Kouta Morinaga ◽  
Vien Viet Luu ◽  
...  
Keyword(s):  
Boar Semen
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