Air-liquid interface cell culture: From airway epithelium to the female reproductive tract

2019 ◽  
Vol 54 ◽  
pp. 38-45 ◽  
Author(s):  
Shuai Chen ◽  
Jennifer Schoen
Keyword(s):  
Cell Culture ◽  
Airway Epithelium ◽  
Reproductive Tract ◽  
Liquid Interface ◽  
Female Reproductive Tract ◽  
Interface Cell ◽  
Air Liquid Interface

A Janus-paper PDMS platform for air–liquid interface cell culture applications

2015 ◽  
Vol 25 (5) ◽  
pp. 055015 ◽  
Author(s):  
Rahim Rahimi ◽  
Manuel Ochoa ◽  
Amy Donaldson ◽  
Tejasvi Parupudi ◽  
Mehmet R Dokmeci ◽  
...  
Keyword(s):  
Cell Culture ◽  
Liquid Interface ◽  
Interface Cell ◽  
Air Liquid Interface

scholarly journals Carbendazim induces death in alveolar epithelial cells: A comparison between submerged and at the air-liquid interface cell culture

2019 ◽  
Vol 58 ◽  
pp. 78-85 ◽  
Author(s):  
Bruna Ferreira Tollstadius ◽  
Artur Christian Garcia da Silva ◽  
Bruna Cristiane Oliveira Pedralli ◽  
Marize Campos Valadares
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Liquid Interface ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Interface Cell ◽  
Air Liquid Interface

scholarly journals Evaluation of an air–liquid interface cell culture model for studies on the inflammatory and cytotoxic responses to tobacco smoke aerosols

2015 ◽  
Vol 29 (7) ◽  
pp. 1720-1728 ◽  
Author(s):  
David Azzopardi ◽  
Linsey E. Haswell ◽  
Geoff Foss-Smith ◽  
Katherine Hewitt ◽  
Nathan Asquith ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tobacco Smoke ◽  
Liquid Interface ◽  
Cell Culture Model ◽  
Culture Model ◽  
Interface Cell ◽  
Air Liquid Interface ◽  
Cytotoxic Responses

scholarly journals A new cell culture exposure system for studying the toxicity of volatile chemicals at the air–liquid interface

2018 ◽  
Vol 30 (4-5) ◽  
pp. 169-177 ◽  
Author(s):  
Jose Zavala ◽  
Allen D. Ledbetter ◽  
David S. Morgan ◽  
Lisa A. Dailey ◽  
Earl Puckett ◽  
...  
Keyword(s):  
Cell Culture ◽  
Liquid Interface ◽  
Exposure System ◽  
Volatile Chemicals ◽  
Air Liquid Interface

Toxicological validation during the development of new catalytic systems using air/liquid interface cell exposure system

2015 ◽  
Vol 238 (2) ◽  
pp. S193
Author(s):  
Y. Landkocz ◽  
M. Al Zallouha ◽  
J. Brunet ◽  
R. Cousin ◽  
J.M. Halket ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Catalytic Systems ◽  
Exposure System ◽  
Interface Cell ◽  
Air Liquid Interface

scholarly journals PHAIR – A biosensor for pH measurement in air-liquid interface

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Neda Saraei ◽  
Gang Xu ◽  
Abiram Chandiramohan ◽  
Jonas Yeung ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Silver Chloride ◽  
Reference Electrode ◽  
Airway Epithelial Cells ◽  
Liquid Interface ◽  
Small Scale ◽  
Human Airway ◽  
Air Liquid Interface

1AbstractIn many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR - a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air-liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenging conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wires-based sensors have the capacity for miniaturization, and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings.

scholarly journals Direct Transcriptomic Comparison of Xenobiotic Metabolism and Toxicity Pathway Induction of Airway Epithelium Models at an Air Liquid Interface Generated from Induced Pluripotent Stem Cells and Primary Bronchial Epithelial Cells

2021 ◽  
Author(s):  
Ivo Djidrovski ◽  
Maria Georgiou ◽  
Elena Tasinato ◽  
Martin O. Leonard ◽  
Jelle Van den Bor ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelium ◽  
Xenobiotic Metabolism ◽  
Liquid Interface ◽  
Inhalation Toxicity ◽  
Induced Pluripotent ◽  
Air Liquid Interface ◽  
Airway Model

Abstract The airway epithelium represents the main barrier between inhaled air and the tissues of the respiratory tract and is therefore an important point of contact with xenobiotic substances into the human body. Several studies have recently shown that in vitro models of the airway grown at an air liquid interface (ALI) can be particularly useful to obtain mechanistic information about the toxicity of chemical compounds. However, such methods are not very amenable to high throughput since the primary cells cannot be expanded indefinitely in culture to obtain a sustainable number of cells. Induced pluripotent stem cells (iPSCs) have become a popular option in the recent years for modelling the airways of the lung, but despite progress in the field, such models have so far not been assessed for their ability to metabolise xenobiotic compounds and how they compare to the primary bronchial airway model (pBAE). Here we report a comparative analysis by TempoSeq (oligo directed sequencing) of an iPSC derived airway model (iBAE) with a primary bronchial airway model (pBAE). The iBAE and pBAE were differentiated at an ALI and then evaluated in a 5-compound screen with exposure to a sub lethal concentration of each compound for 24 hours. We found that despite lower expression of xenobiotic metabolism genes, that the iBAE similarly predicted the toxic pathways when compared to the pBAE model. Our results show that iPSC airway models at ALI show promise for inhalation toxicity assessments with further development.

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