Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis in bovine ovarian granulosa cells

2019 ◽  
Vol 54 (5) ◽  
pp. 741-749 ◽  
Author(s):  
Dejun Xu ◽  
Huanshan He ◽  
Xiaohan Jiang ◽  
Lulu Yang ◽  
Dinbang Liu ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Hormone ◽  
Signalling Pathways ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells

miR-130a-3p regulates steroid hormone synthesis in goat ovarian granulosa cells by targeting the PMEPA1 gene

2021 ◽  
Vol 165 ◽  
pp. 92-98
Author(s):  
Lu Zhu ◽  
Jing Jing ◽  
Shuaiqi Qin ◽  
Qi Zheng ◽  
Jiani Lu ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Hormone ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells

scholarly journals The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells

2018 ◽  
Vol 19 (7) ◽  
pp. 2028 ◽  
Author(s):  
Shujuan Wang ◽  
Wenju Liu ◽  
Xunsheng Pang ◽  
Sifa Dai ◽  
Guodong Liu
Keyword(s):  
Granulosa Cells ◽  
Regulatory Mechanism ◽  
Steroid Hormone ◽  
Inhibin B ◽  
Critical Approach ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells ◽  
Genes Expression ◽  
Significant Difference

Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of MT2 silencing on steroids synthesis, it was found that the expression of CYP19A1 and CYP11A1 enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (StAR, RUNX2, INHA and INHBB) were up-regulated without affecting the expression of INHBA, suggesting that MT2 silencing may regulate hormone abundance. Furthermore, MT2 silencing significantly increased the expression of TGFBR3 and BMP6, and decreased the expression of LHR and DNMT1A without significant difference in the expression of FSHR and EGFR. In addition, MT2 silencing didn’t affect the effect of melatonin on increasing the expression of DNMT1A, EGFR, INHBA and LHR, and progesterone level, or decreasing INHA, TGFBR3 and StAR expression, and production of inhibin B. Moreover, MT2 silencing could disrupt the role of melatonin in decreasing the FSHR, INHBB and BMP6 expression, and activin B secretion. In conclusion, these results reveal that melatonin and MT2 are essential regulator of bovine GCs function by modulating reproduction-related genes expression, hormones secretion and other regulators of folliculogenesis.

RAF1 as Downstream Molecule Mediates the FSH Signaling Pathway to Stimulate E2 Synthesis and Secretion in Mouse Ovarian Granulosa Cells

2020 ◽  
Author(s):  
Xuan Luo ◽  
Hui Liu ◽  
Hongzhou Guo ◽  
Longjie Sun ◽  
Kemian Gou ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Granulosa Cells ◽  
Regulatory Function ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells ◽  
Erk Phosphorylation ◽  
Extracellular Signal ◽  
Key Factor ◽  
Cytochrome P450 Family ◽  
Estradiol Synthesis

Abstract Background: V-raf-leukemia viral oncogene 1 (RAF1) kinase is the key factor in extracellular signal regulated pathway, which transmits signals to the downstream extracellular regulated protein kinases (ERK). Regulatory function of RAF1 has been proved to mediate steroid hormone synthesis, which played an essential physiological function in reproduction and development. Whether RAF1 takes part in the signaling events of gonadotropic hormones follicle-stimulating hormone (FSH) in ovarian is unknown.Results: We found that RAF1 as downstream molecule mediates the FSH signaling pathway to stimulate estradiol (E2) synthesis and secretion in mouse ovarian granulosa cells (GCs). The expression of RAF1 is induced by FSH and the production of E2 is increased in the serum and primary ovarian GCs supernatant, the process of which is blocked by treating with RAF1 inhibitor (N-(2-Methyl-5'-morpholino-6'-((tetrahydro-2H-pyran-4-yl)oxy)-[3,3'-bipyridin]-5-yl)-3(trifluoromethyl) benzamide, RAF709). Inhibition of RAF1 activity by RAF709 decreased ERK phosphorylation, and suppressed the expression of cytochrome P450 family 19 subfamily a member 1 (CYP19A1) which is a major rate-limiting enzyme to participate in the last step of E2 biosynthesis. Conclusion: Our results suggest that RAF1 play a pivotal mediating roles toward E2 production in FSH signaling pathway by inducing the phosphorylation of ERK and promoting the process of estradiol synthesis. RAF1 may be a potential and effective factor to regulate the function of the female mouse reproductive system.

T-2 toxin regulates steroid hormone secretion of rat ovarian granulosa cells through cAMP-PKA pathway

2015 ◽  
Vol 232 (3) ◽  
pp. 573-579 ◽  
Author(s):  
Jing Wu ◽  
Di Tu ◽  
Li-Yun Yuan ◽  
Jin-e Yi ◽  
Yanan Tian
Keyword(s):  
Granulosa Cells ◽  
Steroid Hormone ◽  
Hormone Secretion ◽  
Ovarian Granulosa Cells ◽  
Pka Pathway

MiR-31 and miR-143 affect steroid hormone synthesis and inhibit cell apoptosis in bovine granulosa cells through FSHR

2019 ◽  
Vol 123 ◽  
pp. 45-53 ◽  
Author(s):  
Zhe Zhang ◽  
Cheng-Zhen Chen ◽  
Ming-Qiang Xu ◽  
Li-Qun Zhang ◽  
Jian-Bo Liu ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Steroid Hormone ◽  
Hormone Synthesis

Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells

2019 ◽  
Vol 495 ◽  
pp. 646-651 ◽  
Author(s):  
Xiaowei Nie ◽  
Wenjie Sheng ◽  
Daorong Hou ◽  
Qiang Liu ◽  
Ronggen Wang ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Hormone ◽  
Hormone Secretion ◽  
Ovarian Granulosa Cells
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