Impact of Buserelin Acetate or hCG Administration on the Day of First Artificial Insemination on Subsequent Luteal Profile and Conception Rate in Murrah Buffalo (Bubalus bubalis)

2016 ◽  
Vol 51 (4) ◽  
pp. 478-484 ◽  
Author(s):  
AK Pandey ◽  
SPS Ghuman ◽  
GS Dhaliwal ◽  
SK Agarwal ◽  
JB Phogat
Keyword(s):  
Artificial Insemination ◽  
Bubalus Bubalis ◽  
Conception Rate ◽  
Murrah Buffalo ◽  
Buserelin Acetate

scholarly journals Administration of GnRH on the day of fixed-time artificial insemination (FTAI) and melengestrol acetate (MGA) administration after ftai in non-suckling nelore cattle

2015 ◽  
Vol 36 (5) ◽  
pp. 3149
Author(s):  
Rossane Pereira da Silva ◽  
Karen Martins Leão ◽  
Moraima Castro Rodrigues ◽  
Thaisa Campos Marques ◽  
Natalia Do Carmo Silva ◽  
...  
Keyword(s):  
Oral Administration ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Mineral Supplementation ◽  
Melengestrol Acetate ◽  
Buserelin Acetate ◽  
Conception Rates ◽  
Nelore Cattle

<p>The present study evaluated the effect of administering buserelin acetate (GnRH) at the time of fixed-time artificial insemination (FTAI), along with the effect of oral administration of melengestrol acetate (MGA) after FTAI on conception rates in non-suckling Nelore cattle. In Experiment I, the effect of GnRH application at the time of FTAI was evaluated, as was administration of MGA from the 13th through the 18th day following FTAI (D24 to D29 after the initiation of the FTAI protocol). Experiment I was performed in 215 non-suckling cattle of the Nelore breed, divided into four experimental groups: Control group: 56 cows subjected to FTAI without GnRH injection; GnRH group: 51 cows subjected to GnRH at the time of FTAI; MGA Group: 57 cows subjected to FTAI without the application of GnRH with mineral supplementation and the addition of 2.28 g of MGA® Premix per cow per day from D24 to D29 after the initiation of the FTAI protocol; MGA and GnRH group: 51 cows subjected to GnRH injection at the time of FTAI, with mineral supplementation and the addition of 2.28 g of MGA® Premix per cow per day from D24 to D29 after the initiation of the FTAI protocol. In Experiment II, the effect of providing MGA five to ten days after FTAI was evaluated in 196 non-suckling pluriparous Nelore cows, divided into two groups: Control group: 104 cows subjected to FTAI, not supplemented with MGA; treated group: 92 cows supplemented with the addition of 2.28 g of MGA® Premix per cow per day from D15 to D20 after the initiation of the FTAI protocol. Diagnosis of gestation was carried out 45 days after FTAI. Both experiments were conducted using a completely randomised design and analysed via the SAS MIXED procedure. In Experiment I, the control group displayed a lower conception rate (32.1%) compared with the groups administered GnRH, MGA, and both MGA and GnRH, which were respectively provided with MGA after FTAI (45.6%), GnRH at the time of FTAI (50.9%), and the combination of the two treatments (50.9%) (P&lt;0.05). In Experiment II, the control group displayed a higher conception rate (40.38%) compared with the group receiving MGA post-FTAI (18.48%) (P&lt;0.05). It was concluded that the injection of buserelin acetate (GnRH) at the time of FTAI and the oral administration of MGA® Premix from the 13th to the 18th day after FTAI increased the conception rates in non-suckling Nelore cattle. However, when MGA® Premix was administered orally from the fifth to tenth day post-FTAI, conception rates in non-suckling Nelore cattle were reduced.</p>

scholarly journals Effects of Buserelin acetate and semen extension on fertility in rabbit does under artificial insemination

2020 ◽  
Vol 44 (1) ◽  
pp. 130-135
Author(s):  
E. O. Ewuola ◽  
G. A. Omotosho ◽  
A. A. Adeyemi
Keyword(s):  
Artificial Insemination ◽  
Ovulation Induction ◽  
Conception Rate ◽  
Gestation Length ◽  
Dilution Ratio ◽  
Litter Weight ◽  
Semen Collection ◽  
Buserelin Acetate ◽  
Semen Extender ◽  
Artificial Vagina

The productivity of rabbit farms can be increased and become more homogeneous through the use of Artificial Insemination (AI). However, this possibility is limited with low conception in artificially inseminated does without ovulation induction. This study aimed at determining the conception rate of rabbit does induced with buserelin acetate and inseminated with extended semen at varied dilution ratio. A total number of Twenty eight multiparous non-lactating rabbit does were randomly allotted into four treatments housed individually in a completely randomised design. Rabbits were used as a teaser for semen collection using an artificial vagina and after semen collection and evaluation; ejaculates from ten bucks with more than 60% motility were pooled and extended. Forty eight hours before the AI, the does were hormonally synchronized (i.m) for oestrus with 20IU PMSG. Does in treatment 1(control) were inseminated with unextended semen without buserelin but injected with normal saline, while does on treatments 2, 3 and 4 were inseminated with extended semen in ratio 1: 1, 1:2 and 1: 3 (semen: extender), respectively and were all intramuscularly induced using 0.8µg of buserelin after insemination. Results showed that some of the does induced with buserelin acetate intramuscularly (treatments 2, 3 and 4) were pregnant, while none of the control rabbits was pregnant. The ratio 1: 1 extended semen (treatment 2) recorded the highest percentage conception (85.71%) followed by treatment 3 (71.43%) and treatment 4 (57.14%). There was significant (P<0.05) difference in gestation length (32.50, 31.00 and 32.75), litter size (4.83, 8.60 and 3.25), live kits at birth (3.50, 6.60 and 1.75) for treatments 2, 3 and 4 respectively and the average litter weight was not significantly different among the treatments. This study suggests that diluting semen in ratio 1: 1 produced highest conception rate with intramuscular administration of 0.8µg buserelin  acetate than extending semen in ratios 1: 2 and 1: 3.

scholarly journals Influence of seasonality, timing of insemination and rectal temperature on conception rate of crossbred dairy cows

2016 ◽  
Vol 37 (1) ◽  
pp. 155 ◽  
Author(s):  
Fransérgio Rocha de Souza ◽  
Carla Cristian Campos ◽  
Natascha Almeida Marques da Silva ◽  
Ricarda Maria dos Santos
Keyword(s):  
Dairy Cows ◽  
Rectal Temperature ◽  
Artificial Insemination ◽  
Dairy Farm ◽  
Fixed Time ◽  
Conception Rate ◽  
Digital Thermometer ◽  
Pregnancy Diagnosis ◽  
Chi Squared

This study aimed to evaluate the effects of rectal temperature (RT) on conception rate (CR), as well as the effects of seasonality (spring-summer vs. autumn-winter) and timing of artificial insemination (AI) (morning vs. afternoon) on RT and CR in crossbred dairy cows (Holstein x Gyr). The experiment was conducted on a dairy farm in Centralina, MG, where 1,219 conventional and fixed-time inseminations were analyzed. The RT was measured immediately before AI using a digital thermometer. Pregnancy diagnosis was performed using ultrasonography between 28 and 60 days after AI. T The effects of seasonality and timing of AI on RT were analyzed with a Mann-Whitney U test and the effects of RT (above or below the average), seasonality and timing of AI on CR were analyzed with a Chi-squared test, both using the SAS program. The RT average was 39.4°C. Cows with RT ? 39.4°C had lower CR than cows with RT < 39.4°C (25.78% vs. 32.54%; P = 0.0096). During spring-summer, cows had higher RT (39.44°C ± 0.025 vs. 39.27°C ± 0.022; P < 0.0001) and lower CR (25.49% vs. 31.75%; P = 0.0146) compared with autumn-winter. Cows inseminated in the morning had lower RT (38.96°C ± 0.022 vs. 39.60°C ± 0.018; P < 0.0001) and higher CR (32.86% vs. 26.06%; P = 0.0102) than cows inseminated in the afternoon. In conclusion, crossbred dairy cows with rectal temperature equal to or greater than 39.4°C had lower conception rate. Moreover, rectal temperature and conception rate were affected by seasonality and insemination time.

scholarly journals Conception rate of artificially inseminated Holstein cows affected by cloudy vaginal mucus, under intense heat conditions

2015 ◽  
Vol 50 (6) ◽  
pp. 492-498 ◽  
Author(s):  
Miguel Mellado ◽  
Laura Maricela Lara ◽  
Francisco Gerardo Veliz ◽  
María Ángeles de Santiago ◽  
Leonel Avendaño-Reyes ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Study Data ◽  
Meteorological Conditions ◽  
High Ambient Temperature ◽  
Conception Rate ◽  
Holstein Cows ◽  
Mean Annual Temperature ◽  
Northern Mexico ◽  
Prevalence Estimates ◽  
Intense Heat

The objective of this work was to obtain prevalence estimates of cloudy vaginal mucus in artificially inseminated Holstein cows raised under intense heat, in order to assess the effect of meteorological conditions on its occurrence during estrus and to determine its effect on conception rate. In a first study, an association was established between the occurrence of cloudy vaginal mucus during estrus and the conception rate of inseminated cows (18,620 services), raised under intense heat (mean annual temperature of 22°C), at highly technified farms, in the arid region of northern Mexico. In a second study, data from these large dairy operations were used to assess the effect of meteorological conditions throughout the year on the occurrence of cloudy vaginal mucus during artificial insemination (76,899 estruses). The overall rate of estruses with cloudy vaginal mucus was 21.4% (16,470/76,899; 95% confidence interval = 21.1-21.7%). The conception rate of cows with clean vaginal mucus was higher than that of cows with abnormal mucus (30.6 vs. 22%). Prevalence of estruses with cloudy vaginal mucus was strongly dependent on high ambient temperature and markedly higher in May and June. Acceptable conception rates in high milk-yielding Holstein cows can only be obtained with cows showing clear and translucid mucus at artificial insemination.

scholarly journals The Relationship between Farmer Characteristic and Knowledge towards the Successfulness of Cattle Artificial Insemination (AI) in Aceh Besar District

2018 ◽  
Vol 3 (1) ◽  
pp. 35-40
Author(s):  
Julia Kardin ◽  
Dasrul Dasrul ◽  
Sugito Sugito ◽  
Nurliana Nurliana ◽  
Teuku Zahrial Helmi
Keyword(s):  
Direct Observation ◽  
Artificial Insemination ◽  
Conception Rate ◽  
Farmer Knowledge ◽  
Real Relationship ◽  
Relationship Of ◽  
The Relationship ◽  
General Guidance

The purpose of this research was to identify the relationship of characteristic and knowledge of farmers to the successful of artificial insemination (AI) in Aceh Besar Districts. The number of respondents is 93 people from twenty three districts in Aceh Besar, selected purposively as the sample of this research. The instruments used in this research are questionnaire and direct observation in the field, while the observed variables are: farmer characteristics, farmer knowledge and artificial insemination result. The results of this research revealed that the characteristics and knowledge of the farmers were good and showed a real relationship to the successfulness of AI in Aceh Besar Districts. The average service per conception (S / C) in Aceh Besar Districts during 2017 was 1.32 while the conception rate (CR) was 86.36%. The results were in accordance with the standard of general guidance of Upsus Siwab Year 2017.

scholarly journals KUALITAS SPERMATOZOA ASAL CAPUT, CORPUS, DAN CAUDA EPIDIDIMIS PADA KERBAU RAWA (Bubalus bubalis carabenensis)

2016 ◽  
Vol 3 (3) ◽  
pp. 97
Author(s):  
Muhammad Riyadhi ◽  
Akbar Budiansa ◽  
Muhammad Rizal
Keyword(s):  
Artificial Insemination ◽  
Bubalus Bubalis ◽  
Spermatozoa Motility ◽  
Swamp Buffaloes ◽  
Swamp Buffalo

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.

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