Colour Doppler Ultrasonography as a Tool to Assess Luteal Function in Santa Inês Ewes

2015 ◽  
Vol 50 (4) ◽  
pp. 643-650 ◽  
Author(s):  
LM Figueira ◽  
JF Fonseca ◽  
EKN Arashiro ◽  
JMG Souza-Fabjan ◽  
ACS Ribeiro ◽  
...  
Keyword(s):  
Doppler Ultrasonography ◽  
Colour Doppler ◽  
Luteal Function ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Successive in vivo embryo production in Santa Inês sheep

2020 ◽  
Vol 60 (4) ◽  
pp. 497 ◽  
Author(s):  
Pedro Henrique Nicolau Pinto ◽  
Mario Felipe Alvarez Balaro ◽  
Helena Fabiana Reis de Almeida Saraiva ◽  
Viviane Lopes Brair ◽  
Vivian Angélico Pereira Alfradique ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Doppler Ultrasonography ◽  
Corpora Lutea ◽  
Colour Doppler ◽  
Embryo Production ◽  
Previous Response ◽  
Embryo Recovery ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P < 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P < 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P < 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, >10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

scholarly journals Caliber-Persistent Labial Artery: A Report of a Rarely Recognized Lip Swelling

2018 ◽  
Vol 8 ◽  
pp. 41
Author(s):  
Malarkodi Thanthoni ◽  
P Rajeev ◽  
S Sathasivasubramanian
Keyword(s):  
Doppler Ultrasonography ◽  
Clinical Importance ◽  
Surgical Excision ◽  
Excisional Biopsy ◽  
Vascular Lesion ◽  
Colour Doppler ◽  
Non Invasive ◽  
Colour Doppler Ultrasonography ◽  
Soft Tissue Swelling ◽  
Arterial Branch

Calibre-persistent labial artery (CPLA) is a commonly underdiagnosed vascular lesion of the lip. CPLA is an arterial branch that penetrates the submucosal tissue without loss of calibre. Clinical diagnosis is significant as misdiagnosis can lead to profuse haemorrhage following an excisional biopsy or surgical excision. Colour Doppler ultrasonography is a safe and non-invasive diagnostic tool to confirm the diagnosis. Here, we report a case of a 24-year-old man who complained of an asymptomatic pulsating non-progressive nodule on the left side of upper lip initially diagnosed as peripheral angiomatous lesion. Diagnosis was confirmed by high-resolution Colour Doppler Ultrasonography. The purpose of this case report is to highlight the clinical importance and diagnosis of a rarely reported soft tissue swelling of the lip to the attention of clinicians.

122 Colour-Doppler ultrasonography as a tool for oocyte donor selection

2021 ◽  
Vol 33 (2) ◽  
pp. 169
Author(s):  
F. Villaseñor-González ◽  
H. Álvarez-Gallardo ◽  
M. Kjelland ◽  
A. Velázquez-Roque ◽  
S. Romo
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Doppler Ultrasonography ◽  
Body Condition Score ◽  
P Value ◽  
Colour Doppler ◽  
Colour Doppler Ultrasonography ◽  
Oocyte Donors ◽  
Intravaginal Device ◽  
Selection Of

Colour-Doppler ultrasonography (CDU) has been applied for a more detailed examination of the ovary and uterus, mainly local blood flow in ovarian follicles and the corpus luteum (CL). The main use of CDU is for the diagnosis of early pregnancy or the selection of recipients for embryo transfer in cattle; however, this tool can also be used to select oocyte donors according to blood flow to the ovary. The concept is that more blood flow in the ovary is conducive to a healthier intraovarian environment (e.g. higher progesterone levels and removal of reactive oxygen species), yielding higher quality oocytes. The objective of this research was to evaluate invitro embryo production (IVP) after selection of oocyte donors, considering ovary irrigation before ovum pickup (OPU). The research was carried out in the reproduction laboratory at the Palominos Ranch (Jalisco, México). The oocyte donors (n=15) were synchronized before each round of OPU using an intravaginal device (1.9g of progesterone) with oestradiol benzoate (2mg) and cloprostenol sodium (500µg) on Day 0, to avoid the presence of a corpus luteum and to synchronize the follicular wave. On Day 6, the intravaginal device was removed and OPU was performed. All Angus breed donors between 3 and 5 years old with a body condition score between 5–6 (scale 1–9, where 1 is extremely thin and 9 is very obese) and were evaluated with transrectal CDU (Sonoscape S2™) with a linear-array probe (7.5MHz) before the first OPU session only. The oocyte donors were classified subjectively into three categories: low blood flow (LBF, ∼30% ovarian area), median blood flow (MBF, ∼50% ovarian area), and high blood flow (HBF, ∼70% ovarian area) and were submitted to 3 cycles of IVP each (45 total cycles). Semen from a proven bull for IVF was used during all IVP cycles. All oocytes collected from each donor were used in IVF with the same semen in all IVP cycles. The total oocytes collected, and percentages of viable oocytes, cleavage, and blastocysts on Day 7 of culture were evaluated. Statistical analysis was carried out using the GLM procedure of SAS software (version 9.3; SAS Institute Inc.) to evaluate the results of LBF, MBF, and HBF (P-value=0.05). Total oocyte recovery was 11.28±1.92, 10.06±1.31, and 15.52±1.05 for HBF, MBF, and LBF, respectively, being significantly higher for LBF (P<0.05). There were no differences in viable oocytes among groups. Cleavage rates were 53.04%±3.43 for HBF, 43.18%±2.34 for MBF, and 43.69%±1.89 for LBF, being significantly higher for HBF (P<0.05). Percentage of blastocysts on Day 7 was 38.16%±3.80 for HBF, 30.11%±2.60 for MBF, and 17.78%±2.10 for LBF. This value tended to be significantly higher for HBF than MBF, and both were significantly superior to LBF (P<0.05). In conclusion, under the conditions of this research, although LBF donors had more total recovered oocytes, blastocyst rates were increased in HBF and MBF donors. Therefore, based on the results of the present study, CDU can be a useful tool for the selection of oocyte donors.

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