Induction of Ram Bone Marrow Mesenchymal Stem Cells into Germ Cell Lineage using Transforming Growth Factor-β Superfamily Growth Factors

2014 ◽  
Vol 49 (4) ◽  
pp. 588-598 ◽  
Author(s):  
M Ghasemzadeh-Hasankolaei ◽  
MA Sedighi-Gilani ◽  
MB Eslaminejad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Germ Cell ◽  
Transforming Growth Factor ◽  
Cell Lineage ◽  
Transforming Growth Factor Β ◽  
Marrow Mesenchymal Stem Cells

Allogeneic mesenchymal stem cells and growth factors in gel scaffold repair osteochondral defect in rabbit

2020 ◽  
Vol 15 (2) ◽  
pp. 1261-1275 ◽  
Author(s):  
Mudasir Bashir Gugjoo ◽  
Amarpal ◽  
Ahmed Abdelbaset-Ismail ◽  
Hari Prasad Aithal ◽  
Prakash Kinjavdekar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Collagen Gel ◽  
Transforming Growth Factor Β ◽  
Saline Group ◽  
Group B ◽  
Group D

Aim: An attempt was made to improve osteochondral healing with allogeneic mesenchymal stem cells (MSCs) along with certain growth factors. Materials & methods: Induced knee osteochondral defects were filled as: phosphate buffer saline (group A); MSCs in collagen gel (group B); group B plus insulin like growth factor-1 (group C); group C plus transforming growth factor β-1 (group D). Results: Gross and scanning electron microscopy showed superior morphology and surface architecture of the healed tissue in groups D and C. Histologically, group D revealed hyaline cartilage characteristic features followed in order by group C and group B. In all treatment groups, chondrogenic matrix, collagen II2B ( col II 2B) and aggrecan were secreted. Conclusion: Combined use of MSCs and growth factors could accelerate osteochondral healing.

scholarly journals Proteomic Profiling of Bone Marrow Mesenchymal Stem Cells upon Transforming Growth Factor β1 Stimulation

2004 ◽  
Vol 279 (42) ◽  
pp. 43725-43734 ◽  
Author(s):  
Daojing Wang ◽  
Jennifer S. Park ◽  
Julia S. F. Chu ◽  
Ari Krakowski ◽  
Kunxin Luo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Proteomic Profiling ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Differentiation of Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-Like Cells Is Regulated by the Combined Low Dose Treatment of Transforming Growth Factor-β1 and 5-Azacytidine

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Shutian Shi ◽  
Xingxin Wu ◽  
Xiao Wang ◽  
Wen Hao ◽  
Huangtai Miao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Treatment Group ◽  
Low Dose ◽  
Troponin I ◽  
Transforming Growth Factor ◽  
Dose Treatment ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMMSCs) are used in cardiac tissue engineering for the regeneration of diseased hearts. We examined the differentiation of rat BMMSCs into cardiomyocyte-like cells when induced with a combined low dose treatment of transforming growth factor-β1 (TGF-β1) and 5-azacytidine (5-AZA). Results showed that cell proliferation in the combined low dose treatment group of TGF-β1 and 5-AZA was increased compared with the TGF-β1 group or the 5-AZA group. The cell apoptosis was relieved by combined TGF-β1 and 5-AZA treatment compared to 5-AZA treatment alone. The number of cells positive for myosin heavy chain, connexin-43,α-actin, and troponin I in the combined treatment group was higher than those observed in the TGF-β1 group or the 5-AZA group. Moreover, the combined low dose treatment group of TGF-β1 and 5-AZA reveals the strongest expression of troponin I,α-actin, and phosphorylated extracellular signal-regulated protein kinases 1 and 2 (p-ErK1/2) among the treatment groups. These results suggest that the combined low dose treatment of TGF-β1 and 5-AZA can improve the differentiation potential of rat BMMSCs into cardiomyocyte-like cells and alleviate cell damage effectsin vitro. The mechanism that is involved in influencing differentiation may be associated with p-ErK1/2.

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