scholarly journals A literature review as an aid to identify strategies for mitigating ostreid herpesvirus 1 in Crassostrea gigas hatchery and nursery systems

2018 ◽  
Vol 11 (3) ◽  
pp. 565-585 ◽  
Author(s):  
Chris Rodgers ◽  
Isabelle Arzul ◽  
Noèlia Carrasco ◽  
Dolores Furones Nozal
Keyword(s):  
Literature Review ◽  
Crassostrea Gigas ◽  
Herpesvirus 1 ◽  
Ostreid Herpesvirus 1

scholarly journals Dual Analysis of Virus-Host Interactions: The Case of Ostreid herpesvirus 1 and the Cupped Oyster Crassostrea gigas

2019 ◽  
Vol 15 ◽  
pp. 117693431983130
Author(s):  
Umberto Rosani ◽  
Tim Young ◽  
Chang-Ming Bai ◽  
Andrea C. Alfaro ◽  
Paola Venier
Keyword(s):  
Crassostrea Gigas ◽  
Current Knowledge ◽  
Expression Profiles ◽  
Defense Responses ◽  
Viral Disease ◽  
Complex Case ◽  
Rna Seq ◽  
Omics Technologies ◽  
Herpesvirus 1 ◽  
Ostreid Herpesvirus 1

Dual analyses of the interactions between Ostreid herpesvirus 1 (OsHV-1) and the bivalve Crassostrea gigas during infection can unveil events critical to the onset and progression of this viral disease and can provide novel strategies for mitigating and preventing oyster mortality. Among the currently used “omics” technologies, dual transcriptomics (dual RNA-seq) coupled with the analysis of viral DNA in the host tissues has greatly advanced the knowledge of genes and pathways mostly contributing to host defense responses, expression profiles of annotated and unknown OsHV-1 open reading frames (ORFs), and viral genome variability. In addition to dual RNA-seq, proteomics and metabolomics analyses have the potential to add complementary information, needed to understand how a malacoherpesvirus can redirect and exploit the vital processes of its host. This review explores our current knowledge of “omics” technologies in the study of host-pathogen interactions and highlights relevant applications of these fields of expertise to the complex case of C gigas infections by OsHV-1, which currently threaten the mollusk production sector worldwide.

scholarly journals Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Umberto Rosani ◽  
Miriam Abbadi ◽  
Timothy Green ◽  
Chang-Ming Bai ◽  
Edoardo Turolla ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Messenger Rna ◽  
Regulatory Networks ◽  
Viral Infections ◽  
Degradation Products ◽  
Differentially Expressed ◽  
Protein Coding ◽  
Functional Studies ◽  
Herpesvirus 1 ◽  
Ostreid Herpesvirus 1

Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks.

scholarly journals In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas

2016 ◽  
Vol 136 ◽  
pp. 124-135 ◽  
Author(s):  
Claire Martenot ◽  
Amélie Segarra ◽  
Laury Baillon ◽  
Nicole Faury ◽  
Maryline Houssin ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Herpesvirus 1 ◽  
Ostreid Herpesvirus 1
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