Proliferating cell nuclear antigen and Vasa protein expression during gonadal development and sexual differentiation in cultured Siberian (Acipenser baeriiBrandt, 1869) and Russian (Acipenser gueldenstaedtiiBrandt & Ratzeburg, 1833) sturgeon

2013 ◽  
Vol 6 (2) ◽  
pp. 75-88 ◽  
Author(s):  
Malgorzata Rzepkowska ◽  
Teresa Ostaszewska
Keyword(s):  
Protein Expression ◽  
Sexual Differentiation ◽  
Proliferating Cell Nuclear Antigen ◽  
Gonadal Development ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Proliferating cell nuclear antigen (PCNA), p53 and MDM2 expression in Hodgkin’s disease

2007 ◽  
Vol 125 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Gevina Silva Pinheiro ◽  
Maria Regina Régis Silva ◽  
Celso Arrais Rodrigues ◽  
José Kerbauy ◽  
José Salvador Rodrigues de Oliveira
Keyword(s):  
Protein Expression ◽  
Hodgkin's Disease ◽  
Proliferating Cell Nuclear Antigen ◽  
Hodgkin’S Disease ◽  
Prognostic Significance ◽  
Sao Paulo ◽  
Nuclear Antigen ◽  
São Paulo ◽  
Proliferation Markers ◽  
Proliferating Cell

CONTEXT AND OBJECTIVE: Tumor cells in Hodgkin’s disease (HD) express cell proliferation markers that are evaluated according to the oncogenes involved or the expression of their proteins. Correlations between the protein expression grade and clinical data are now important for disease prognosis. DESIGN AND SETTING: This was a retrospective analysis on proliferating cell nuclear antigen (PCNA), p53 and MDM2 (murine double minute-2) expression using immunohistochemistry, on formalin-fixed, paraffin-embedded tissues from diagnostic biopsies on 51 patients with HD. The study was conducted at the Division of Hematology and Transfusion Medicine, Hospital São Paulo, Universidade Federal de São Paulo. METHODS: Antigen expression was evaluated as the proportions of positive Hodgkin and Reed-Sternberg (HRS) cells and reactive lymphocytes (L), which were compared using Spearman correlation coefficients. The Friedman test was used for comparisons between the markers. The Pearson test was used to investigate associations between marker expression and clinical and laboratory parameters, marrow involvement, complete remission (CR) and overall survival (OS) rates. RESULTS: There was overexpression of antigen proteins in HRS, in relation to L (p < 0.001). In HRS, MDM2 was higher than p53 and PCNA (p < 0.003), while the latter two were equivalent. In L, p53 was lower than MDM2 and PCNA (p < 0.001), while the latter two were equivalent. There was no relationship between protein expression and clinical and laboratory variables or outcome. CONCLUSIONS: PCNA, p53 and MDM2 are tumor markers for HD, but showed no clinical or prognostic significance in our analysis.

scholarly journals Up-Regulation by IGF-I of Proliferating Cell Nuclear Antigen and Bcl-2 Protein Expression in Human Uterine Leiomyoma Cells

2001 ◽  
Vol 86 (11) ◽  
pp. 5593-5599 ◽  
Author(s):  
Zhijian Gao ◽  
Hiroya Matsuo ◽  
Yin Wang ◽  
Satoshi Nakago ◽  
Takeshi Maruo
Keyword(s):  
Protein Expression ◽  
Mtt Assay ◽  
Proliferating Cell Nuclear Antigen ◽  
Immunoblot Analysis ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Immunocytochemical Staining ◽  
Phenol Red ◽  
Igf I ◽  
Proliferating Cell

IGF-I has been reported to play a role in regulating proliferation of human leiomyoma cells. There is, however, little evidence to suggest that IGF-I inhibits apoptosis in the leiomyoma cells. The present study was conducted to elucidate whether IGF-I affects apoptosis and Bcl-2 protein expression, an apoptosis-inhibiting gene product, in cultured leiomyoma cells. In addition, we examined the effect of IGF-I on proliferating cell nuclear antigen (PCNA) expression in cultured leiomyoma cells. Isolated human leiomyoma cells were subcultured in phenol red-free DMEM supplemented with 10% FBS for 120 h and then stepped down to serum-free conditions for an additional 72 h in the absence or presence of graded concentrations of IGF-I (1.0, 10, and 100 ng/ml). The effects of IGF-I on Bcl-2 protein and PCNA expression in cultured leiomyoma cells were assessed by Western immunoblot analysis and immunocytochemical staining, whereas the effects of IGF-I on the cell viability and apoptosis of the cultured cells were determined by 3-(4,5-dimethylatriazol-2-yl)-2,5diphenyltetrasodium bromide (MTT) assay and terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate nick end labeling assay, respectively. Immunocytochemical staining demonstrated that IGF-I treatment resulted in the increase in PCNA labeling index in cultured leiomyoma cells in a dose-dependent manner. Immunoblot analysis of proteins extracted from the cultured leiomyoma cells revealed that the addition of IGF-I (10 and 100 ng/ml) significantly increased the expression of 35-kDa immunoreactive PCNA and 26-kDa Bcl-2 protein, compared with those in control cultures. Cell survival and proliferation of cultured leiomyoma cells, assessed by MTT assay, was significantly augmented by IGF-I treatment, compared with those of control cultures. Terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate nick end labeling assay showed that the apoptosis-positive rate of leiomyoma cells treated with IGF-I was significantly decreased, compared with that in control cultures. The present results suggest that IGF-I plays crucial roles in leiomyoma cell growth, not only in promoting the proliferative potential by up-regulation of PCNA expression but also in down-regulating apoptosis by up-regulation of Bcl-2 protein expression in leiomyoma cells.

scholarly journals Effect of dietary corn silage inclusion on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers

2009 ◽  
Vol 89 (4) ◽  
pp. 503-512 ◽  
Author(s):  
Y J Wang ◽  
K M Wood ◽  
L Martin ◽  
S Holligan ◽  
N Kelly ◽  
...  
Keyword(s):  
Protein Expression ◽  
Atp Synthase ◽  
Proliferating Cell Nuclear Antigen ◽  
Corn Silage ◽  
Nuclear Antigen ◽  
Visceral Organ ◽  
Visceral Mass ◽  
Significant Treatment ◽  
Organ Mass ◽  
Proliferating Cell

Twenty-four steers (initial body weight = 535 ± 5.0 kg) predominately of Angus breeding were used to determine the effect of dietary corn silage inclusion [20, 40, 60, or 80% of dry matter (DM)] on visceral mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. Steers were fed at similar energy levels (2.1 × NEm requirement). There were no significant treatment effects on specific visceral organ weights. Hepatic Na+/K+-ATPase expression linearly increased (P = 0.01) and ruminal Na+/K+-ATPase expression linearly decreased (P = 0.01) with increasing corn silage inclusion. Hepatic PCNA expression was quadratically affected (P = 0.05) with a decrease when corn silage inclusion increased from 20 to 60%, and an increase when corn silage inclusion increased from 60 to 80%. Renal ATP synthase (P = 0.02) and ubiquitin expression (P = 0.01) were quadratically affected in a similar pattern with an increase when corn silage inclusion increased from 20 to 60%, and a decrease when corn silage inclusion increased from 60 to 80%. These results indicate that different dietary corn silage inclusions, at similar dietary energy intake, may alter rumen, liver, and kidney energy expenditure, at least in part, through changes in specific metabolism rather than mass. Key words: Corn silage inclusion, visceral organ mass, cellular energy metabolism, steer

Effect of dietary crude protein level on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers

2009 ◽  
Vol 89 (4) ◽  
pp. 493-501 ◽  
Author(s):  
Y J Wang ◽  
S Holligan ◽  
H Salim ◽  
M Z Fan ◽  
B W McBride ◽  
...  
Keyword(s):  
Small Intestine ◽  
Protein Expression ◽  
Atp Synthase ◽  
Proliferating Cell Nuclear Antigen ◽  
Crude Protein ◽  
Nuclear Antigen ◽  
Visceral Organ ◽  
Organ Mass ◽  
Dietary Crude Protein ◽  
Proliferating Cell

Twenty-four steers [initial body weight (BW) = 510 ± 4.9 kg] predominately of Angus breeding were used to determine the effect of dietary crude protein (CP) level [8.5, 10.7, 12.3 or 14.5%, dry matter (DM) basis; high-moisture-corn-based diets] on visceral mass, cellularity, and protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. Steers were on dietary treatment for 28 d. Kidney, liver, and reticulorumen weights (g) increased linearly (P < 0.05) with increased dietary CP. Lung weight (g; g kg-1 BW) linearly increased (P < 0.05) with increased CP. Ruminal and small intestinal DNA concentration, and the ratios of RNA:DNA and protein:DNA in small intestine were affected quadratically by increased dietary CP (P < 0.05). Hepatic ATP synthase expression was affected quadratically with an increase when dietary CP increased from 8.5 to 10.7%, and a decrease when dietary CP increased from 10.7 to 14.5% (P < 0.05). Renal ATP synthase expression decreased linearly (P < 0.05) and small intestine mucosal Na+/K+-ATPase expression increased linearly (P = 0.05) with increased CP. These results indicate that increasing dietary CP increases liver, kidney, lung, and rumen masses, and differentially influences expression of proteins influencing energy utilization and efficiency in liver, kidney, and small intestine.Key words: Dietary crude protein, visceral organ mass, cellular energy metabolism, steer

Sign in / Sign up

Export Citation Format

Share Document