Genome editing using a DNA ‐free clustered regularly interspaced short palindromic repeats ‐Cas9 system in green seaweed Ulva prolifera

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

Journal of Ocean University of China ◽

10.1007/s11802-019-3687-y ◽

2019 ◽

Vol 18 (1) ◽

pp. 185-192 ◽

Cited By ~ 2

Author(s):

Jiaxin Li ◽

Pei Zhang ◽

Liang Hong ◽

Chenguang Liu

Keyword(s):

Ulva Prolifera ◽

Purification And Characterization ◽

Green Seaweed

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Integration of white shrimp (Litopenaeus vannamei) and green seaweed (Ulva prolifera) in minimum-water exchange aquaculture system

Journal of Applied Phycology ◽

10.1007/s10811-018-1601-4 ◽

2018 ◽

Vol 31 (2) ◽

pp. 1425-1432 ◽

Cited By ~ 6

Author(s):

Hong-xing Ge ◽

Qian Ni ◽

Jian Li ◽

Ji-tao Li ◽

Zhao Chen ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Water Exchange ◽

Ulva Prolifera ◽

White Shrimp ◽

Aquaculture System ◽

Green Seaweed

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Asexual thalli originated from sporophytic thalli via apomeiosis in the green seaweed Ulva

Scientific Reports ◽

10.1038/s41598-019-50070-x ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Kensuke Ichihara ◽

Tomokazu Yamazaki ◽

Shinichi Miyamura ◽

Masanori Hiraoka ◽

Shigeyuki Kawano

Keyword(s):

Mating Type ◽

Asexual Reproduction ◽

Ulva Prolifera ◽

Transcriptomic Analysis ◽

Reproductive Cell ◽

Reproduction System ◽

Green Seaweed ◽

Asexual Populations ◽

And Behavior

Abstract Apomixis is an asexual reproduction system without fertilization, which is an important proliferation strategy for plants and algae. Here, we report on the apomeiosis in the green seaweed Ulva prolifera, which has sexual and obligate asexual populations. Genomic PCR of mating type (MT)-locus genes revealed asexual thalli carrying both MT genomes. Observation of the chromosomes during the formation of each type of reproductive cell revealed that cells in asexual thalli performed apomeiosis without chromosome reduction. Moreover, genotyping revealed that laboratory-cultured sporophytic thalli produced not only each type of gametophyte but also diploid thalli carrying the mt− and mt+ genome (mt± thallus strains). The mt± thallus strain released diploid biflagellate zoids, with ultrastructure and behavior similar to mt+ gametes. Additionally, a transcriptomic analysis revealed that some meiosis-related genes (Mei2L and RAD1) were highly expressed in the quadriflagellate zoosporoids. Our results strongly suggest that asexual thalli originally evolved via apomeiosis in sporophytic thalli.

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