Alteration of fatty acid chain length ofChlamydomonas reinhardtiiby simultaneous expression of medium-chain-specific thioesterase and acyl carrier protein

2017 ◽  
Vol 65 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Yu Inaba ◽  
Kenji Nakahigashi ◽  
Takuro Ito ◽  
Masaru Tomita
Keyword(s):  
Fatty Acid ◽  
Chain Length ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Medium Chain ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain ◽  
Simultaneous Expression

scholarly journals Overexpression of the Plant Medium-chain Acyl-carrier Protein Thioesterase BTE for the Overproduction of the nC14-surfactin Isoform with Promising MEOR Applications

2021 ◽  
Author(s):  
fangxiang hu ◽  
Weijie Cai ◽  
Junzhang Lin ◽  
Weidong Wang ◽  
Shuang Li
Keyword(s):  
Fatty Acid ◽  
Hydroxy Fatty Acid ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Medium Chain ◽  
Emulsification Index ◽  
Fatty Acid Chain ◽  
Umbellularia Californica ◽  
Chain Acyl ◽  
Surfactin Production

Abstract BackgroundSurfactin, a representative biosurfactant of popeptide mainly produced by Bacillus subtilis, consists of a cyclic heptapeptide linked to a β-hydroxy fatty acid chain. The functional activity of surfactin is closely related to the length and isomerism of the fatty acid chain. ResultsIn this study, the plant medium-chain acyl-carrier protein (ACP) thioesterase (BTE) from Umbellularia californica was overexpressed in a recombinant surfactin production strain based on B. subtilis 168. As a result, the surfactin yield after 24 h of cultivation improved by 23%, and the production rate increased from 0.112 to 0.177 g/L/h. The isoforms identified by RP-HPLC and GC-MS showed that the proportion of nC14-surfactin increased 6.4 times compared to the control strain. A comparison of further properties revealed that the product with more nC14-surfactin had higher surface activity and better performance in oil-washing. Finally, the product with more nC14-surfactin isoform had a higher hydrocarbon-emulsification index, and it increased the water-wettability of the oil-saturated silicate surface. ConclusionThe obtained results provide an original approach to modify the fatty acid chain of surfactin and further demonstrate the importance of the length and isomerism of the β-hydroxy fatty acid chain for the MEOR application of surfactin.

Satiation in humans is modulated by fatty acid chain length

2001 ◽  
Vol 120 (5) ◽  
pp. A710-A710
Author(s):  
S LAL ◽  
J MCLAUGHLIN ◽  
O NIAZ ◽  
G DOCKRAY ◽  
A VARRO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chain Length ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain

scholarly journals Kinetic studies on pancreatic lipase activity in micellar systems. II. Effect of fatty acid chain length of substrates.

1983 ◽  
Vol 31 (12) ◽  
pp. 4213-4219 ◽  
Author(s):  
HIROSHI NAKAHARA ◽  
SATOSHI OKADA ◽  
KENSHU MOCHIDA ◽  
HIDENOBU OHMORI ◽  
MASAICHIRO MASU
Keyword(s):  
Fatty Acid ◽  
Chain Length ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Kinetic Studies ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain ◽  
Micellar Systems

Abundance of sphingomyelins inversely correlates with N-acyl fatty acid chain length in pancreatic cancer

Pancreatology ◽  
2020 ◽  
Vol 20 ◽  
pp. S41
Author(s):  
U. Mahajan ◽  
G. Adam ◽  
A. Alnatsha ◽  
Q. Li ◽  
G. Beyer ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Fatty Acid ◽  
Chain Length ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain

Effect of glycosphingolipid fatty acid chain length on behavior in unsaturated phosphatidylcholine bilayers: A deuterium NMR study

Biochemistry ◽  
1993 ◽  
Vol 32 (1) ◽  
pp. 290-297 ◽  
Author(s):  
Dalian Lu ◽  
Dev Singh ◽  
Michael R. Morrow ◽  
Chris W. M. Grant
Keyword(s):  
Fatty Acid ◽  
Chain Length ◽  
Deuterium Nmr ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain ◽  
Nmr Study
Sign in / Sign up

Export Citation Format

Share Document