ACC deaminase‐producing Brevibacterium linens RS16 enhances heat‐stress tolerance of rice ( Oryza sativa L.)

UV-B radiation and high temperature have detrimental effects on plant physiological and biochemical processes. The use of bacterial inoculants for stress alleviation has been regarded as a sustainable and eco-friendly approach. Hence, this study was conducted to evaluate the ability of 1-aminocyclopropane-1-caboxylate (ACC) deaminase-producing Brevibacterium linens RS16 in enhancing stress tolerance in rice against combined UV-B radiation and heat stresses. A combination of 0.5 Wm−2 UV-B radiation and 40 °C of temperature were imposed on rice plants for 5 days. The plants imposed with combined stress had shown significantly higher ethylene emissions compared to the plants grown under normal conditions. In addition, the stress imposition had shown negative effects on the photosynthetic traits, biomass, and genetic material of rice plants. The inoculation of bacteria had shown a 26.5% and 31.8% decrease in ethylene emissions at 3 and 4 days of stress imposition compared to the non-inoculated plants. Additionally, bacterial inoculation had also enhanced plant biomass and photosynthetic traits, and had proved to be effective in restricting DNA damage under stress conditions. Taken together, the current study has shown the effective strategy of enhancing stress tolerance against the interactive effects of UV-B radiation and heat stresses by regulation of ethylene emissions through inoculating ACC deaminase-producing bacteria.

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Premature Senescence Leaf 50 Promotes Heat Stress Tolerance in Rice (Oryza Sativa L.)

10.21203/rs.3.rs-116224/v1 ◽

2020 ◽

Author(s):

Yan He ◽

Xiao-bo Zhang ◽

Yongfeng Shi ◽

Xia Xu ◽

Liangjian Li ◽

...

Keyword(s):

Oryza Sativa ◽

Heat Stress ◽

Leaf Senescence ◽

Oryza Sativa L ◽

Premature Senescence ◽

Causal Gene ◽

Senescent Leaf ◽

Heat Stress Tolerance ◽

Premature Leaf Senescence ◽

Leaf Mutants

Abstract BackgroundHeat stress is a major environmental factor that could induce premature leaf senescence in plants. So far, few rice premature senescent leaf mutants have been reported to involve in heat tolerance.FindingsWe identified a premature senescence leaf 50 (psl50) mutant that exhibited a higher heat susceptibility with decreased survival rate, over-accumulated hydrogen peroxide (H2O2) content and increased cell death under heat stress compared with the wild-type. The causal gene PREMATURE SENESCENCE LEAF 50 (PSL50) was isolated by using initial map-based resequencing (IMBR) approach, and we found that PSL50 promoted heat tolerance probably by acting as a modulator of H2O2 signaling in response to heat stress in rice (Oryza sativa L.).ConclusionsPSL50 negatively regulates heat-induced premature leaf senescence in rice.

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PREMATURE SENESCENCE LEAF 50 Promotes Heat Stress Tolerance in Rice (Oryza sativa L.)

Rice ◽

10.1186/s12284-021-00493-w ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yan He ◽

Xiaobo Zhang ◽

Yongfeng Shi ◽

Xia Xu ◽

Liangjian Li ◽

...

Keyword(s):

Oryza Sativa ◽

Heat Stress ◽

Leaf Senescence ◽

Heat Tolerance ◽

Oryza Sativa L ◽

Premature Senescence ◽

Senescent Leaf ◽

Heat Stress Tolerance ◽

Premature Leaf Senescence ◽

Leaf Mutants

Abstract Background Heat stress is a major environmental factor that could induce premature leaf senescence in plants. So far, a few rice premature senescent leaf mutants have been reported to involve in heat tolerance. Findings We identified a premature senescence leaf 50 (psl50) mutant that exhibited a higher heat susceptibility with decreased survival rate, over-accumulated hydrogen peroxide (H2O2) content and increased cell death under heat stress compared with the wild-type. The causal gene PREMATURE SENESCENCE LEAF 50 (PSL50) was isolated by using initial map-based resequencing (IMBR) approach, and we found that PSL50 promoted heat tolerance probably by acting as a modulator of H2O2 signaling in response to heat stress in rice (Oryza sativa L.). Conclusions PSL50 negatively regulates heat-induced premature leaf senescence in rice.

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