Transcriptome ‐ wide isolation and expression of NF‐Y gene family in male cone development and hormonal treatment of Pinus tabuliformis

2020 ◽  
Vol 171 (1) ◽  
pp. 34-47
Author(s):  
Yingtian Guo ◽  
Shihui Niu ◽  
Yousry A. El‐Kassaby ◽  
Wei Li
Keyword(s):  
Gene Family ◽  
Hormonal Treatment ◽  
Male Cone ◽  
Pinus Tabuliformis ◽  
Cone Development

scholarly journals Transcriptome-wide isolation and expression profiles of NF-Y gene family in male cone development and hormone treatment of Chinese pine (Pinus tabuliformis)

2020 ◽  
Author(s):  
Yingtian Guo ◽  
Shihui Niu ◽  
Wei Li
Keyword(s):  
Phylogenetic Relationships ◽  
Expression Profiles ◽  
Hormone Treatment ◽  
Male Cone ◽  
Nuclear Factor Y ◽  
Conserved Regions ◽  
Pinus Tabuliformis ◽  
Cone Development

Abstract Background Conifers and angiosperms have difference in reproductive development, especially for flowering. It is known that NUCLEAR FACTOR Y (NF-Y) transcription factor play an important role in flowering, drought stress and GA, ABA signaling, but, little known in auxin, salicylic acid, jasmonic acid, etc. Moreover, the NF-Y genes family has been mainly analyzed in angiosperms, but it has not been comprehensively reported in conifers. Results In this study, we identified 9 NF-YA, 9 NF-YB, and 10 NF-YC genes in Pinus tabuliformis using Arabidopsis NF-Y protein sequences as queries. Besides, by comparing conserved regions and phylogenetic relationships of the PtNF-Ys, we found that the NF-Ys were both conserved and altered during evolution. PtTFL2, PtCO, PtNF-YC1 and PtNF-YC4 were exploited by expression profile in male cone development and the correlation analysis. In addition, NF-YC1/4 can interact with DPL by yeast two-hybrid assays and BiFC. The multiple types of phytohormones-responsive cis-elements (ABA, JA, IAA, SA) were present and many NF-Y genes responded positively to SA and as opposed to IAA and JA. Conclusions Twenty-eight PtNF-Ys were identified and bioinformatic characterization of NF-Y genes including conserved regions, phylogenetic relationships, gene-motifs, was carried out. Two candidate genes (NF-YC1 and NF-YC4) were found to be involved in the regulation of conifer flowering and gibberellin signalling. The cis-elements and hormone transcriptome analysis revealed that the potential role of NF-Ys in conifers resistance. This study provides the basis for improved understanding of NF-Y genes function in conifers.

scholarly journals Morpho-Histology, Endogenous Hormone Dynamics and Transcriptome Profiling in Dacrydium Pectinatum during Male Cone Development

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1598
Author(s):  
Wenju Lu ◽  
Enbo Wang ◽  
Weijuan Zhou ◽  
Yifan Li ◽  
Zhaoji Li ◽  
...  
Keyword(s):  
Pollen Mother Cell ◽  
Mother Cell ◽  
Cell Formation ◽  
Transcriptome Profiling ◽  
Endogenous Hormone ◽  
Male Cone ◽  
Transcriptome Database ◽  
Cone Development ◽  
Hormone Biosynthesis ◽  
External Forces

Dacrydium pectinatum de Laubenfels is a perennial gymnosperm species dominant in tropical montane rain forests. Due to severe damages by excessive deforestation, typhoons, and other external forces, the population of the species has been significantly reduced. Furthermore, its natural regeneration is poor. To better understand the male cone development in D. pectinatum, we examined the morphological and anatomical changes, analyzed the endogenous hormone dynamics, and profiled gene expression. The morpho-histological observations suggest that the development of D. pectinatum male cone can be largely divided into four stages: microspore primordium formation (April to May), microspore sac and pollen mother cell formation (July to November), pollen mother cell division (January), and pollen grain formation (February). The levels of gibberellins (GA), auxin (IAA), abscisic Acid (ABA), cytokinin (CTK), and jasmonic acid (JA) fluctuated during the process of male cone development. The first transcriptome database for a Dacrydium species was generated, revealing >70,000 unigene sequences. Differential expression analyses revealed several floral and hormone biosynthesis and signal transduction genes that could be critical for male cone development. Our study provides new insights on the cone development in D. pectinatum and the foundation for male cone induction with hormones and studies of factors contributing to the species’ low rate of seed germination.

Temporal and Spatial Characteristics Endogenous Hormone Regulation During Male Cone Development of Dacrydium pectinatum

2020 ◽  
Author(s):  
Wenju Lu ◽  
Zhaoji Li ◽  
Xiqiang Song ◽  
Jian Wang ◽  
Mingxun Ren ◽  
...  
Keyword(s):  
Pollen Grains ◽  
Endogenous Hormone ◽  
Hormone Regulation ◽  
Rain Forests ◽  
Reproductive Process ◽  
Male Cone ◽  
Cone Development ◽  
Temporal And Spatial ◽  
Temporal And Spatial Characteristics ◽  
External Forces

Abstract Background: Dacrydiumpectinatum de Laubenfels is a perennial gymnosperm dominant in tropical montane rain forests. Due to severe damages by excessive deforestation, typhoons, and other external forces, the population of the species has been significantly reduced. Furthermore, natural regeneration is poor. In order to better understand the reproductive process in D. pectinatum, we examined the morphological and anatomical changes during the development of male cone and analyzed the endogenous hormone dynamics.Results: Our study indicates that D. pectinatum male buds become distinguishable in April in tropical montane rain forests, while microspore sac forms in September and pollen mother cell forms and divides in December. Pollen grains mature and disperse in the following February. A mature male cone averages 8.5 mm in length. Level of GA, IAA, ABA and JA and their ratios fluctuated during late August to late November when sporogenous tissues were actively differentiated.Conclusions: The differentiation of sporogenous tissues is accompanied by variations in levels of endogenous hormones (GA, ABA, IAA, and JA) and their balances. The new insights about the cone development in D. pectinatum lay the foundation for future cone induction with hormones and study of factors contributing to the species’ low rate of seed germination.

scholarly journals Temporal and spatial characteristics of male cone development inMetasequoia glyptostroboidesHu et Cheng

2012 ◽  
Vol 7 (12) ◽  
pp. 1687-1694 ◽  
Author(s):  
Biao Jin ◽  
Liang Tang ◽  
Yan Lu ◽  
Di Wang ◽  
Min Zhang ◽  
...  
Keyword(s):  
Spatial Characteristics ◽  
Male Cone ◽  
Cone Development ◽  
Temporal And Spatial ◽  
Temporal And Spatial Characteristics

Hormonal Treatment in Cancer

1950 ◽  
Vol 34 (5) ◽  
pp. 1409-1417 ◽  
Author(s):  
Ira T. Nathanson
Keyword(s):  
Hormonal Treatment

Hormonal stimulation and some risk factors studied to improve bovine pregnancy rate at the Coastal areas of Barisal district of Bangladesh

2018 ◽  
Vol 52 (1-4) ◽  
pp. 22-30
Author(s):  
A K Paul ◽  
S M T Rahman
Keyword(s):  
Pregnancy Rate ◽  
Coastal Region ◽  
Hormonal Treatment ◽  
Coastal Areas ◽  
Pregnancy Rates ◽  
Hormonal Stimulation ◽  
Crossbred Cows ◽  
Treatment Groups ◽  
History Of ◽  
Group A

Hormonal treatment of cows at the coastal region of Barisal district of Bangladesh was performed to assess the improvement of pregnancy rate. A total of 100 cows and heifers with irregular history of cyclicity were selected randomly and divided into five treatment groups. The groups were A (treated with anthelmintic), B (treated with anthelmintic, vitamin ADE and multivitamin powder), C (treated with PGF2α), D (treated with GnRH) and E (treated with GnRH and PGF2α). Each group comprised of 20 animals. The age, breed and parity of experimental cows were considered during treatment. In the study, the cows treated with both GnRH and PGF2α (group E) showed significantly (p<0.05) higher estrus (80%) and pregnancy rate (60%) than that of group A, B, C and D. The overall estrus rates of local and crossbred cows were 64% and 70%, respectively and the pregnancy rates were 40 and 52%, respectively. The crossbred cows responded significantly (p<0.05) to hormonal treatment than that of local cows. Parity-2 cows showed higher estrus sign than that of other parities. However, the pregnancy rates were higher significantly (p<0.05) in parity-2 and parity ≥4 cows than that of parity-0, parity-1 and parity-3 cows. The pregnancy rate was also found higher in case of 4 to <5 years old cows than that of 2 to <3, 3 to <4, 5 to <6, and ≥6 years old. It may conclude that the hormonal regimen increases the pregnancy rate as well as decreases the undesired waiting of estrus and conception. Further study with more sample size will reveal the more effective treatment for cows at the coastal areas of Bangladesh.

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