Fe 2 O 3 nanoparticles induced biochemical responses and expression of genes involved in rosmarinic acid biosynthesis pathway in Moldavian balm under salinity stress

2020 ◽  
Vol 169 (4) ◽  
pp. 555-570 ◽  
Author(s):  
Hanieh Moradbeygi ◽  
Rashid Jamei ◽  
Reza Heidari ◽  
Reza Darvishzadeh
Keyword(s):  
Salinity Stress ◽  
Rosmarinic Acid ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Biochemical Responses ◽  
Expression Of Genes

scholarly journals Effect of graphene / metal nanocomposites on the key genes involved in rosmarinic acid biosynthesis pathway and its accumulation in Melissa officinalis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Roya Karimi Soraki ◽  
Mahyar Gerami ◽  
Moazzameh Ramezani
Keyword(s):  
Gene Expression ◽  
Silver Nanoparticle ◽  
Rosmarinic Acid ◽  
Phenylalanine Ammonia Lyase ◽  
Tyrosine Aminotransferase ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Low Concentration ◽  
Key Genes ◽  
Rosmarinic Acid Synthase

Abstract Background Recently, numerous investigations have been done to study graphene and silver nanoparticle in the fields of agriculture and medicine. In the present study, the green synthesis of nanoparticles with two concentrations (0, 40, 60 mM) and their effect on the molecular and biochemical biosynthesis pathway of rosmarinic acid in a new method, low cost, and safe for the environment has been investigated. The transcript levels of key genes in the rosmarinic acid biosynthesis pathway (Tyrosine aminotransferase, rosmarinic acid synthase, and phenylalanine-ammonia lyase) were studied using real-time quantitative polymerase chain reaction. Then, the rosmarinic acid content was evaluated using HPLC. Results The results showed that a concentration-dependent manner was observed in treated plants. At the biochemical level, the use of nanocomposites at concentration of 40 mM showed higher soluble carbohydrate (37%), flavonoids (21%), total phenol (35%) as well as total protein (47%) compared to the control plants. HPLC results showed that rosmarinic acid content in the treated plants with a low concentration of nanocomposite (40 mM) was more affected than plants treated with a high concentration of nanocomposite (60 mM) (26%) and also compared to other treatments. At the molecular level, the result showed that Tyrosine aminotransferase and rosmarinic acid synthase gene expression was positively correlated with both silver nanoparticle concentrations and nanocomposite treatments, but phenylalanine-ammonia lyase gene expression was positively correlated only with nanocomposite at 40 mM concentration. Conclude It can conclude that the nanocomposite at low concentration is more likely to induce molecular and biochemical parameters. And also, in the rosmarinic acid biosynthesis pathway, the Tyrosine aminotransferase -derived pathway is more efficient than the phenylalanine-ammonia lyase -derived pathway by causing a nano-elicitor. Therefore, it was concluded that studied elicitor at low concentration, can create plants with higher production capacity.

scholarly journals The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Wei Zhou ◽  
Min Shi ◽  
Changping Deng ◽  
Sunjie Lu ◽  
Fenfen Huang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Methyl Jasmonate ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Salvia Miltiorrhiza ◽  
Water Soluble ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Expression Of Genes ◽  
Genes Encoding

AbstractWater-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza. Phenolic acid biosynthesis is induced by methyl jasmonate (MeJA) in this important Chinese herb. Here, we investigated the mechanism underlying this induction by analyzing a transcriptome library of S. miltiorrhiza in response to MeJA. Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1. Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway, including cytochrome P450-dependent monooxygenase (CYP98A14). Dual-luciferase (dual-LUC) assays and/or an electrophoretic mobility shift assays (EMSAs) indicated that SmMYB1 activated the expression of CYP98A14, as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes, including chalcone isomerase (CHI) and anthocyanidin synthase (ANS). In addition, SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone. Taken together, these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S. miltiorrhiza. These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S. miltiorrhiza.

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