Plastidial starch phosphorylase is highly associated with starch accumulation process in developing squash ( Cucurbita sp.) fruit

2019 ◽  
Vol 167 (2) ◽  
pp. 264-275 ◽  
Author(s):  
Shinji Mizuno ◽  
Yusuke Kamiyoshihara ◽  
Hajime Shiba ◽  
Fumie Shinmachi ◽  
Keiichi Watanabe ◽  
...  
Keyword(s):  
Starch Accumulation ◽  
Accumulation Process ◽  
Starch Phosphorylase

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

Damage accumulation near a hole under low cycle fatigue proceeding from measurements of local deformation response

2020 ◽  
Vol 86 (10) ◽  
pp. 46-55
Author(s):  
S. I. Eleonsky ◽  
Yu. G. Matvienko ◽  
V. S. Pisarev ◽  
A. V. Chernov
Keyword(s):  
Stress Intensity Factor ◽  
Stress Intensity ◽  
Intensity Factor ◽  
Damage Accumulation ◽  
Stress Ratio ◽  
Low Cycle Fatigue ◽  
Accumulation Process ◽  
Stress Range ◽  
Cycle Fatigue ◽  
Deformation Response

A new destructive method for quantitative determination of the damage accumulation in the vicinity of a stress concentrator has been proposed and verified. Increase of damage degree in local area with a high level of the strain gradient was achieved through preliminary low-cycle pull-push loading of plane specimens with central open holes. The above procedure is performed for three programs at the same stress range (333.3 MPa) and different stress ratio values 0.33, – 0.66 and – 1.0, and vice versa for two programs at the same stress ratio – 0.33 and different stress range 333.3 and 233.3 MPa. This process offers a set of the objects to be considered with different degree of accumulated fatigue damages. The key point of the developed approach consists in the fact that plane specimens with open holes are tested under real operation conditions without a preliminary notching of the specimen initiating the fatigue crack growth. The measured parameters necessary for a quantitative description of the damage accumulation process were obtained by removing the local volume of the material in the form of a sequence of narrow notches at a constant level of external tensile stress. External load can be considered an amplifier enhancing a useful signal responsible for revealing the material damage. The notch is intended for assessing the level of fatigue damage, just as probe holes are used to release residual stress energy in the hole drilling method. Measurements of the deformation response caused by local removing of the material are carried out by electronic speckle-pattern interferometry at different stages of low-cycle fatigue. The transition from measured in-plane displacements to the values of the stress intensity factor (SIF) and the T-stress was carried out on the basis of the relations of linear fracture mechanics. It was shown that the normalized dependences of the stress intensity factor on the durability percentage for the first notch (constructed for four programs of cyclic loading with different parameters), reflect the effect of the stress ratio and stress range of the loading cycle on the rate of damage accumulation. The data were used to obtain the explicit form of the damage accumulation function that quantitatively describes damage accumulation process. The functions were constructed for different stress ratios and stress ranges.

Varietal difference of root development and starch accumulation on stem of tomato occurred by fruit thinning

Root Research ◽  
2012 ◽  
Vol 21 (2) ◽  
pp. 39-43 ◽  
Author(s):  
Akimasa Nakano ◽  
Ryo Matsuda ◽  
Masahumi Johkan ◽  
Katsumi Suzuki ◽  
Donghyuk Ahn ◽  
...  
Keyword(s):  
Root Development ◽  
Varietal Difference ◽  
Starch Accumulation ◽  
Fruit Thinning

Identification and Gene Mapping of Starch Accumulation and Early Senes-cence Leaf Mutant esl9 in Rice

2017 ◽  
Vol 43 (4) ◽  
pp. 473 ◽  
Author(s):  
Yan-Hua XIAO ◽  
Xin-Long CHEN ◽  
Dan DU ◽  
Ya-Di XING ◽  
Tian-Quan ZHANG ◽  
...  
Keyword(s):  
Gene Mapping ◽  
Starch Accumulation ◽  
Leaf Mutant

Differences in Starch Accumulation among Dry Bean Cultivars 1

Crop Science ◽  
1978 ◽  
Vol 18 (1) ◽  
pp. 155-157 ◽  
Author(s):  
M. W. Adams ◽  
J. V. Wiersma ◽  
Julio Salazar
Keyword(s):  
Starch Accumulation ◽  
Dry Bean
Sign in / Sign up

Export Citation Format

Share Document