Transcriptome analysis of strawberry ( Fragaria × ananassa ) fruits under osmotic stresses and identification of genes related to ascorbic acid pathway

2018 ◽  
Vol 166 (4) ◽  
pp. 979-995 ◽  
Author(s):  
Vanessa Galli ◽  
Rafael S. Messias ◽  
Frank Guzman ◽  
Ellen C. Perin ◽  
Rogério Margis ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Transcriptome Analysis ◽  
Fragaria Ananassa ◽  
Acid Pathway

scholarly journals Growth and Antioxidant-Related Effects of the Reestablished Ascorbic Acid Pathway in Zebrafish (Danio rerio) by Genomic Integration of L-Gulonolactone Oxidase From Cloudy Catshark (Scyliorhinus torazame)

2021 ◽  
Vol 12 ◽  
Author(s):  
K. A. S. N. Shanaka ◽  
Sumi Jung ◽  
N. D. Janson ◽  
J. R. P. Jayasingha ◽  
K. P. Madushani ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
System Analysis ◽  
Fluorescent Protein ◽  
Fluorescent Microscopy ◽  
Wild Type ◽  
Cloning Method ◽  
Production Growth ◽  
Gulonolactone Oxidase ◽  
Acid Pathway ◽  
Species Production

Loss of L-gulonolactone oxidase (GULO), which catalyzes the last step of the ascorbic acid (AA) biosynthesis pathway, results in a complete lack of AA in several Osteichthyes fish species, including zebrafish. In this study, sGULO, the active GULO gene from cloudy catshark (Scyliorhinus torazame) was cloned into zebrafish using the Gateway cloning method. The resulting Tg(b-actin:sGULO:mCherry) fish were analyzed for the effects of a reestablished AA pathway. Fluorescent microscopy and PCR were used to analyze the integration of the construct into the zebrafish genome. Catalytic activity of sGULO, AA production, growth-related characteristics, and gene expression were investigated to evaluate the effects of AA production in Tg fish. The mCherry fluorescent protein indicated the proper integration and expression of the sGULO construct in zebrafish. The sGULO gene was ubiquitously expressed in all the studied tissues and the enzyme activity indicated an increased AA production in Tg fish. The growth of Tg fish was also increased, and antioxidant system analysis suggests that reactive oxygen species production was reduced in Tg fish compared with wild type. Expression of the AA transporter slc23a1 was significantly downregulated in Tg homozygous fish. These results collectively indicate the effects of reestablished AA synthesis in zebrafish.

scholarly journals Studies on the effects of chemical preservatives on the quality of strawberry (Fragaria ananassa) juice in Bangladesh

1970 ◽  
Vol 1 (4) ◽  
pp. 97-101 ◽  
Author(s):  
R Sabina ◽  
SH Miyan ◽  
MM Hoque
Keyword(s):  
Ascorbic Acid ◽  
Sodium Benzoate ◽  
Nutritive Value ◽  
Mean Value ◽  
Soluble Solids ◽  
Fragaria Ananassa ◽  
Maximum Increase ◽  
Mean Values ◽  
Chemical Preservatives ◽  
Strawberry Juice

This study was carried out to assess the nutritive value of fresh strawberry (Fragaria ananassa) juice and to observe the effect of chemical preservatives on the acidity, ascorbic acid and total soluble solids (TSS) content of strawberry juice. Strawberry was being collected from the local farm of Sylhet city. The proximate composition of strawberry juice was found to have contents of moisture 89.64%, fat 0.48%, fiber 1.013%, ash 0.53%, Titrable acidity 1.02%, ascorbic acid 120 mg/100g, TSS 6%, and pH 3.7. To observe the effects of chemical preservatives the samples were treated with 30% sucrose, 0.2% sodium benzoate, 0.2% potassium sorbet, mixture of 30% sucrose and 0.2% sodium benzoate, mixture of 30% sucrose and 0.2% potassium sorbet, mixture of 30% sucrose, 0.2% sodium benzoate and 0.2% potassium sorbet. The study was conducted for 3 months; storage on refrigeration temperature and the content of acidity; ascorbic acid and TSS were assessing every 15th day’s interval. The result showed mixture of 30% sucrose, 0.2% sodium benzoate and 0.2% potassium sorbet gives retention to most of the nutrients during 3 months storage. The results showed that the ascorbic acid of the juice was found to be with minimum percentage decrease in sample T6 (6.93%) and maximum in T0 (64.69), on acidity of juice there was no such significant changes thus showing similar mean values in the samples, and TSS of juice recorded maximum mean value in T6 (33.5) and minimum mean value T0 (5.1) where maximum increase was observed in T0 (20%) and minimum in T5 (3.54). The results suggested that the effects of chemical preservatives have significant role in strawberry juice and its livelihood. The changes of ascorbic acid content of strawberry juice for T0 treatment significantly changes with T3 (p=.025), T4 (p=.029), T5 (p=.027), T6 (p=.001).The changes of acidity treated with different preservatives significantly changes with period of time but insignificantly changes with the treatment. The changes of TSS content of strawberry juice significantly changes with treatment. The homogeneous subsets are T0 T2 T4 and the subsets b contain T3, T1, T5 and T6 (p=o.ooo). Among all the treatments T6 were most effective in maintaining the nutritional quality during storage. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9736 IJNS 2011 1(4): 97-101

scholarly journals Two UGT84A Family Glycosyltransferases Regulate Phenol, Flavonoid, and Tannin Metabolism in Juglans regia (English Walnut)

2021 ◽  
Vol 12 ◽  
Author(s):  
Houston J. Saxe ◽  
Takanori Horibe ◽  
Bipin Balan ◽  
Timothy S. Butterfield ◽  
Noah G. Feinberg ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Transgenic Tobacco ◽  
Phenolic Acids ◽  
Transcriptome Analysis ◽  
Shikimic Acid ◽  
Juglans Regia ◽  
Shikimate Dehydrogenase ◽  
Shikimic Acid Pathway ◽  
Acid Pathway

We showed previously that gallic acid is produced in walnut from 3-dehydroshikimate by a shikimate dehydrogenase (JrSkDH). This study focuses on the next step in the hydrolysable tannin pathway, the formation of 1-O-galloyl-β-D-glucose from the phenolic gallic acid and UDP glucose by a glycosyltransferase. JrGGT1 (UGT84A73) and JrGGT2 (UGT84A74) are predicted to be two such glycosyltransferases, which we expressed in tobacco plants. GC-MS analysis of the transgenic tobacco revealed moderate, yet significant alterations in plant secondary metabolism, such as depleted phenolic acids, including gallic acid. We postulate that these effects are due to JrGGT1 and JrGGT2 activity, as JrGGT orthologs glycosylate these phenolic compounds in vitro. Moreover, JrGGT expression in tobacco caused upregulation of shikimic acid pathway metabolites and differing responses in phenylpropanoids, such as phenolic acids and flavonoids. In transcriptome analysis of walnut pellicle tissues, both JrGGTs showed substantial and significant expression correlations with the gallic acid-producing JrSkDHs and were highly coexpressed with the genetic circuits constituting the shikimic acid and phenylpropanoid biosynthetic pathways. Verification of JrGGT gene expression by transcriptome analysis of 20 walnut tissues revealed striking similarities with that of the pellicle data, with the greatest expression in roots, wood, buds, and leaves of Juglans regia cv. Chandler: tissues that typically accumulate hydrolysable tannins. Like the transgenic tobacco, pellicle metabolomic analyses revealed that many phenylpropanoids correlated negatively with JrGGT expression, while shikimic acid pathway metabolites correlated positively with JrGGT expression. This research supports the hypothesis that JrGGT1 and JrGGT2 play non-trivial roles in metabolism of phenolic acids, flavonoids, and ostensibly, tannins.

scholarly journals Ascorbic Acid Content and Transcriptional Profiling of Genes Involved in Its Metabolism during Development of Petals, Leaves, and Fruits of Orange (Citrus sinensis cv. Valencia Late)

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2590
Author(s):  
Enriqueta Alós ◽  
Florencia Rey ◽  
José Vicente Gil ◽  
María Jesús Rodrigo ◽  
Lorenzo Zacarias
Keyword(s):  
Ascorbic Acid ◽  
Citrus Sinensis ◽  
Galacturonic Acid ◽  
Transcriptional Profiling ◽  
Citrus Fruit ◽  
Ascorbic Acid Content ◽  
Cis Acting ◽  
Transcriptional Changes ◽  
Recycling Pathway ◽  
Acid Pathway

Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated.

GENERATIVE AND VEGETATIVE PRODUCTIVITY OF LARGE-FRUITED STRAWBERRY FRAGARIA ANANASSA WHEN USING BIOSTIMULANTS FROM MARINE HYDROBIONTS OF KAMCHATKA

2020 ◽  
pp. 81-92
Author(s):  
T.G. Dakhno ◽  
◽  
O.A. Dakhno ◽  
Keyword(s):  
Ascorbic Acid ◽  
General Condition ◽  
Organic Fertilizer ◽  
Quality Characteristics ◽  
Marine Organisms ◽  
Organic Fertilizers ◽  
Fragaria Ananassa ◽  
Biological Product ◽  
Subsequent Recovery ◽  
Positive Effect

The influence of organic fertilizers (biostimulants) “Bio-Algo”, “Bio-Mix” and “Bio-Fish” made from Kam-chatka’s marine organisms on the indicators of generative and vegetative productivity of large-fruited straw-berries were represented. Comparison of organic biostimulants effect on such indicators as winter hardiness, general condition and pathogen damage revealed that the most effective biological product is “Bio-Algo”, the use of which had a positive effect on the development of strawberry plants and subsequent recovery pro-cesses after overwintering, increasing their generative and vegetative productivity. When processing straw-berry plants with the preparation an increase in the mass of the berry was noted, this ultimately increases generative productivity, without reducing the quality characteristics of the fruit – the content of ascorbic acid and soluble dry substances. It was stated that the use of organic fertilizer Bio-Algo increases the generative productivity of large-fruited strawberries by 1.3 times and the generative productivity by 1.6 times.

scholarly journals Regulation of ascorbic acid and of xylulose synthesis in rat-liver extracts. The effect of alloxan-diabetes on the glucuronic acid pathway

1965 ◽  
Vol 97 (2) ◽  
pp. 561-564 ◽  
Author(s):  
F Stirpe ◽  
M Comporti
Keyword(s):  
Ascorbic Acid ◽  
Insulin Therapy ◽  
Rat Liver ◽  
Alloxan Diabetes ◽  
Glucuronic Acid ◽  
Diabetic Rats ◽  
Acid Pathway

1. The synthesis of l-ascorbic acid and of l-xylulose from d-glucuronolactone, d-glucuronate, l-gulonate and l-gulonolactone has been studied with liver extracts from normal and alloxan-diabetic rats. 2. In diabetic animals the synthesis of ascorbic acid is impaired, and more from glucuronolactone and glucuronate than from gulonate and gulonolactone, whereas the formation of xylulose from gulonate and gulonolactone is enhanced. These changes are reversed by insulin therapy. 3. The activity of the NAD-linked gulonate dehydrogenase is enhanced during diabetes.

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