scholarly journals Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis

2018 ◽  
Vol 163 (2) ◽  
pp. 170-182 ◽  
Author(s):  
Nuoendagula ◽  
Yukiko Tsuji ◽  
Naoki Takata ◽  
Shingo Sakamoto ◽  
Akiko Nakagawa‐Izumi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Lignin Content ◽  
Cell Wall Biosynthesis ◽  
Transgenic Poplar ◽  
Master Regulator ◽  
Secondary Cell Wall Biosynthesis ◽  
Lignin Structure

scholarly journals The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar

2020 ◽  
Vol 21 (11) ◽  
pp. 3993 ◽  
Author(s):  
Shaofeng Li ◽  
Yaoxiang Zhang ◽  
Xuebing Xin ◽  
Changjun Ding ◽  
Fuling Lv ◽  
...  
Keyword(s):  
Cell Wall ◽  
Secondary Wall ◽  
Secondary Cell Wall ◽  
Vascular Tissue ◽  
Lignin Content ◽  
Hybrid Poplar ◽  
Lignin Biosynthesis ◽  
Negative Regulator ◽  
Cell Wall Biosynthesis ◽  
Secondary Cell Wall Biosynthesis

Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated PdOLP1 from Populus deltoides (Marsh.). Expression analysis showed that PdOLP1 transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in P. deltoides. We conducted phenotypic, anatomical, and molecular analyses of PdOLP1-overexpressing lines and the PdOLP1-downregulated hybrid poplar 84K (Populus alba × Populus glandulosa) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to PdOLP1, and are downregulated in PdOLP1-downregulated hybrid poplar 84K). The overexpression of PdOLP1 led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of PdOLP1-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in PdOLP1-downregulated lines. A PdOLP1 coexpression network was constructed and showed that PdOLP1 was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the PdOLP1 promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the PdOLP1 promoter in vivo. Together, our results suggest that PdOLP1 is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.

scholarly journals NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants

2015 ◽  
Vol 6 ◽  
Author(s):  
Yoshimi Nakano ◽  
Masatoshi Yamaguchi ◽  
Hitoshi Endo ◽  
Nur Ardiyana Rejab ◽  
Misato Ohtani
Keyword(s):  
Cell Wall ◽  
Transcriptional Regulation ◽  
Secondary Cell Wall ◽  
Land Plants ◽  
Cell Wall Biosynthesis ◽  
Secondary Cell Wall Biosynthesis

scholarly journals PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yongil Yang ◽  
Chang Geun Yoo ◽  
William Rottmann ◽  
Kimberly A. Winkeler ◽  
Cassandra M. Collins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Secondary Cell Wall ◽  
Lignin Biosynthesis ◽  
Cell Wall Biosynthesis ◽  
Wild Type ◽  
Nac Domain ◽  
Sugar Release ◽  
Secondary Cell Wall Biosynthesis

Abstract Background Plant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species. Results Here we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants. Conclusions PdWND3A affects lignin biosynthesis and composition and negatively impacts sugar release and biomass production.

scholarly journals A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis

2008 ◽  
Vol 20 (10) ◽  
pp. 2763-2782 ◽  
Author(s):  
Ruiqin Zhong ◽  
Chanhui Lee ◽  
Jianli Zhou ◽  
Ryan L. McCarthy ◽  
Zheng-Hua Ye
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Secondary Cell Wall ◽  
Cell Wall Biosynthesis ◽  
Secondary Cell Wall Biosynthesis

scholarly journals Organization of Xylan Production in the Golgi During Secondary Cell Wall Biosynthesis

2019 ◽  
Vol 181 (2) ◽  
pp. 527-546 ◽  
Author(s):  
Miranda J. Meents ◽  
Sanya Motani ◽  
Shawn D. Mansfield ◽  
A. Lacey Samuels
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Cell Wall Biosynthesis ◽  
Secondary Cell Wall Biosynthesis
Sign in / Sign up

Export Citation Format

Share Document