GsSLAH3, a Glycine soja slow type anion channel homolog, positively modulates plant bicarbonate stress tolerance

2018 ◽  
Vol 164 (2) ◽  
pp. 145-162 ◽  
Author(s):  
Xiangbo Duan ◽  
Yang Yu ◽  
Huizi Duanmu ◽  
Chao Chen ◽  
Xiaoli Sun ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Glycine Soja ◽  
Anion Channel ◽  
Slow Type ◽  
Bicarbonate Stress

scholarly journals Anion channel SLAH3 is a regulatory target of chitin receptor-associated kinase PBL27 in microbial stomatal closure

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Yi Liu ◽  
Tobias Maierhofer ◽  
Katarzyna Rybak ◽  
Jan Sklenar ◽  
Andy Breakspear ◽  
...  
Keyword(s):  
Stomatal Closure ◽  
Anion Channel ◽  
Anion Channels ◽  
Slow Type ◽  
Leaf Level ◽  
Short Signal ◽  
Molecular Patterns ◽  
Fungal Immunity ◽  
Anti Fungal ◽  
Full Activation

In plants, antimicrobial immune responses involve the cellular release of anions and are responsible for the closure of stomatal pores. Detection of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) induces currents mediated via slow-type (S-type) anion channels by a yet not understood mechanism. Here, we show that stomatal closure to fungal chitin is conferred by the major PRRs for chitin recognition, LYK5 and CERK1, the receptor-like cytoplasmic kinase PBL27, and the SLAH3 anion channel. PBL27 has the capacity to phosphorylate SLAH3, of which S127 and S189 are required to activate SLAH3. Full activation of the channel entails CERK1, depending on PBL27. Importantly, both S127 and S189 residues of SLAH3 are required for chitin-induced stomatal closure and anti-fungal immunity at the whole leaf level. Our results demonstrate a short signal transduction module from MAMP recognition to anion channel activation, and independent of ABA-induced SLAH3 activation.

Over-expression of a novel JAZ family gene from Glycine soja, increases salt and alkali stress tolerance

2012 ◽  
Vol 426 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Dan Zhu ◽  
Hua Cai ◽  
Xiao Luo ◽  
Xi Bai ◽  
Michael K. Deyholos ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Glycine Soja ◽  
Alkali Stress ◽  
Over Expression ◽  
Family Gene

scholarly journals SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl−accumulation and salt tolerance inArabidopsis thaliana

2016 ◽  
Vol 67 (15) ◽  
pp. 4495-4505 ◽  
Author(s):  
Jiaen Qiu ◽  
Sam W Henderson ◽  
Mark Tester ◽  
Stuart J Roy ◽  
Mathew Gilliham
Keyword(s):  
Salt Tolerance ◽  
Anion Channel ◽  
Slow Type

scholarly journals GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

2020 ◽  
Author(s):  
Jinping Fan ◽  
Jingshuang Liu ◽  
Lei Cao ◽  
Jiayue Wang ◽  
Qingxue Meng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Glycine Soja ◽  
Plant Responses ◽  
Lipid Transfer ◽  
Reading Frame ◽  
Microarray Expression

Abstract Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPS in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans.Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses.Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

scholarly journals GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

2021 ◽  
Author(s):  
Jinping Fan ◽  
Jingshuang Liu ◽  
Lei Cao ◽  
Jiayue Wang ◽  
Qingxue Meng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Glycine Soja ◽  
Plant Responses ◽  
Lipid Transfer ◽  
Reading Frame ◽  
Microarray Expression

Abstract Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPs in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans. Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses. Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

scholarly journals Concentration matters: different stomatal CO2-responses at sub-ambient and above-ambient CO2 levels

2021 ◽  
Author(s):  
Hanna Hõrak ◽  
Kaspar Koolmeister ◽  
Ebe Merilo ◽  
Hannes Kollist
Keyword(s):  
Guard Cell ◽  
Stomatal Closure ◽  
Cell Signalling ◽  
Anion Channel ◽  
Leaf Temperature ◽  
Signalling Pathways ◽  
Stomatal Responses ◽  
Slow Type ◽  
Ambient Concentrations ◽  
Ambient Co2

Stomatal pores, formed of paired guard cells, mediate CO2 uptake for photosynthesis and water loss via transpiration in plants. Globally rising atmospheric CO2 concentration triggers stomatal closure, contributing to increased leaf temperature and reduced nutrient uptake due to lower transpiration rate. Hence, it is important to understand the signalling pathways that control elevated CO2-induced stomatal closure to identify targets for breeding climate-ready crops. CO2-induced stomatal closure can be studied by increasing CO2 concentration from ambient to above-ambient concentrations, or elevation of CO2 levels from sub-ambient to above-ambient. Previous experiments comparing ferns with angiosperms suggested that stomatal responses to CO2 may be different, when changing CO2 levels in the sub-ambient or above-ambient ranges. Here, we set out to test this by comparing CO2-induced stomatal closure in key guard cell signalling mutants in response to CO2 elevation from 100 to 400 ppm or 400 to 800 ppm. We show that signalling components that contribute to CO2-induced stomatal closure are different in the sub-ambient and above-ambient CO2 levels, with guard cell slow-type anion channel SLAC1 involved mainly in above-ambient CO2-induced stomatal closure.

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