Nitric oxide is involved in brassinosteroid-induced alternative respiratory pathway inNicotiana benthamianaseedlings' response to salt stress

2015 ◽  
Vol 156 (2) ◽  
pp. 150-163 ◽  
Author(s):  
Tong Zhu ◽  
Xing-Guang Deng ◽  
Wen-Rong Tan ◽  
Xue Zhou ◽  
Shi-Shuai Luo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Salt Stress ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway

Effect of 24-epibrassinolide on Arabidopsis thaliana alternative respiratory pathway under salt stress

2015 ◽  
Vol 37 (10) ◽  
Author(s):  
Michael Derevyanchuk ◽  
Raisa Litvinovskaya ◽  
Vladimir Khripach ◽  
Jan Martinec ◽  
Volodymyr Kravets
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway

Salt stress-induced expression of rice AOX1a is mediated through an accumulation of hydrogen peroxide

Biologia ◽  
2010 ◽  
Vol 65 (5) ◽  
Author(s):  
Hanqing Feng ◽  
Yifeng Wang ◽  
Hongyu Li ◽  
Rongfang Wang ◽  
Kun Sun ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Salt Stress ◽  
Alternative Oxidase ◽  
Oryza Sativa L ◽  
Transcript Level ◽  
Nacl Stress ◽  
H2o2 Production ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway ◽  
Rice Leaves

AbstractTreatment with 300 mM NaCl increased the capacity of the alternative respiratory pathway and induced the expression of AOX1a of the leaves of rice (Oryza sativa L.) seedlings. A significant increase in the content of hydrogen peroxide (H2O2) was observed in rice leaves treated with 300 mM NaCl. However, NaCl at 150 mM did not significantly affect the capacity of the alternative respiratory pathway, the content of H2O2, and the transcript level of AOX1a. Exogenous application of H2O2 enhanced the levels of the capacity of the alternative respiratory pathway and AOX1a expression. The accumulation of H2O2 in rice leaves in response to 300 mM NaCl was inhibited by the pretreatment with dimethylthiourea (DMTU, scavenger of H2O2). This treatment also suppressed the induction of AOX1a expression and the increase in the capacity of the alternative respiratory pathway under 300 mM NaCl stress. Moreover, the salt-stressed (300 mM NaCl) seedlings pretreated with 1 mM salicylhydroxamic acid (SHAM, a special inhibitor of alternative oxidase) had higher level of H2O2 production than the seedlings either subjected to 300 mM NaCl stress or SHAM treatment alone did. These observations suggest that the expression of AOX1a in response to higher salt stress is mediated through an accumulation of H2O2 and alternative oxidase could play a role in antioxidant protection under the condition of higher salt stress.

Effect of 24-epibrassinolide on Brassica napus alternative respiratory pathway, guard cells movements and phospholipid signaling under salt stress

Steroids ◽  
2017 ◽  
Vol 117 ◽  
pp. 16-24 ◽  
Author(s):  
Michael Derevyanchuk ◽  
Sergii Kretynin ◽  
Oksana Iakovenko ◽  
Raisa Litvinovskaya ◽  
Vladimir Zhabinskii ◽  
...  
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Guard Cells ◽  
Respiratory Pathway ◽  
Phospholipid Signaling ◽  
Alternative Respiratory Pathway

A Functional Alternative Oxidase Modulates Plant Salt Tolerance in Physcomitrella patens

2019 ◽  
Vol 60 (8) ◽  
pp. 1829-1841 ◽  
Author(s):  
Guochun Wu ◽  
Sha Li ◽  
Xiaochuan Li ◽  
Yunhong Liu ◽  
Shuangshuang Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Alternative Oxidase ◽  
Physcomitrella Patens ◽  
Redox Homeostasis ◽  
Respiratory Pathway ◽  
Functional Link ◽  
Salt Stress Condition ◽  
Plant Salt Tolerance

Abstract Alternative oxidase (AOX) has been reported to be involved in mitochondrial function and redox homeostasis, thus playing an essential role in plant growth as well as stress responses. However, its biological functions in nonseed plants have not been well characterized. Here, we report that AOX participates in plant salt tolerance regulation in moss Physcomitrella patens (P. patens). AOX is highly conserved and localizes to mitochondria in P. patens. We observed that PpAOX rescued the impaired cyanide (CN)-resistant alternative (Alt) respiratory pathway in Arabidopsis thaliana (Arabidopsis) aox1a mutant. PpAOX transcription and Alt respiration were induced upon salt stress in P. patens. Using homologous recombination, we generated PpAOX-overexpressing lines (PpAOX OX). PpAOX OX plants exhibited higher Alt respiration and lower total reactive oxygen species accumulation under salt stress condition. Strikingly, we observed that PpAOX OX plants displayed decreased salt tolerance. Overexpression of PpAOX disturbed redox homeostasis in chloroplasts. Meanwhile, chloroplast structure was adversely affected in PpAOX OX plants in contrast to wild-type (WT) P. patens. We found that photosynthetic activity in PpAOX OX plants was also lower compared with that in WT. Together, our work revealed that AOX participates in plant salt tolerance in P. patens and there is a functional link between mitochondria and chloroplast under challenging conditions.

Cloning and Analysis of the Alternative Oxidase Gene of Neurospora crassa

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 129-140 ◽  
Author(s):  
Qiuhong Li ◽  
R Gary Ritzel ◽  
Lesley L T McLean ◽  
Lee McIntosh ◽  
Tak Ko ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Alternative Oxidase ◽  
Electron Flow ◽  
Mitochondrial Protein ◽  
Transcriptional Level ◽  
Wild Type ◽  
Respiratory Pathway ◽  
Oxidase Gene ◽  
Alternative Respiratory Pathway ◽  
Cytochrome Pathway

Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated and analyzed. The sequence of the predicted protein is homologous to that of other species. The mRNA for the alternative oxidase is scarce in wild-type cultures grown under normal conditions, but it is abundant in cultures grown in the presence of chloramphenicol, an inhibitor of mitochondrial protein synthesis, or in mutants deficient in mitochondrial cytochromes. Thus, induction of alternative oxidase appears to be at the transcriptional level. Restriction fragment length polymorphism mapping of the isolated gene demonstrated that it is located in a position corresponding to the aod-1 locus. Sequence analysis of mutant aod-1 alleles reveals mutations affecting the coding sequence of the alternative oxidase. The level of aod-1 mRNA in an aod-2 mutant strain that had been grown in the presence of chloramphenicol was reduced several fold relative to wild-type, supporting the hypothesis that the product of aod-2 is required for optimal expression of aod-1.

scholarly journals Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress

2019 ◽  
Vol 14 (11) ◽  
pp. 1667730 ◽  
Author(s):  
Aditi Tailor ◽  
Rajesh Tandon ◽  
Satish C. Bhatla
Keyword(s):  
Nitric Oxide ◽  
Salt Stress ◽  
Sunflower Seedling

Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis

2019 ◽  
Vol 70 (20) ◽  
pp. 5575-5590 ◽  
Author(s):  
Shan-Shan Wei ◽  
Wei-Tao Niu ◽  
Xiao-Ting Zhai ◽  
Wei-Qian Liang ◽  
Meng Xu ◽  
...  
Keyword(s):  
Alternative Oxidase ◽  
Redox Homeostasis ◽  
Superoxide Dismutase 1 ◽  
Respiratory Pathway ◽  
Cellular Processes ◽  
Growth Defects ◽  
Alternative Respiratory Pathway ◽  
Abnormal Mitochondria ◽  
Shock Proteins ◽  
Severe Growth

Abstract The 70 kDa heat shock proteins function as molecular chaperones and are involved in diverse cellular processes. However, the functions of the plant mitochondrial HSP70s (mtHSC70s) remain unclear. Severe growth defects were observed in the Arabidopsis thaliana mtHSC70-1 knockout lines, mthsc70-1a and mthsc70-1b. Conversely, the introduction of the mtHSC70-1 gene into the mthsc70-1a background fully reversed the phenotypes, indicating that mtHSC70-1 is essential for plant growth. The loss of mtHSC70-1 functions resulted in abnormal mitochondria and alterations to respiration because of an inhibition of the cytochrome c oxidase (COX) pathway and the activation of the alternative respiratory pathway. Defects in COX assembly were observed in the mtHSC70-1 knockout lines, leading to decreased COX activity. The mtHSC70-1 knockout plants have increased levels of reactive oxygen species (ROS). The introduction of the Mn-superoxide dismutase 1 (MSD1) or the catalase 1 (CAT1) gene into the mthsc70-1a plants decreased ROS levels, reduced the expression of alternative oxidase, and partially rescued growth. Taken together, our data suggest that mtHSC70-1 plays important roles in the establishment of COX-dependent respiration.

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