Ultrastructural observation of mesophyll cells and temporal expression profiles of the genes involved in transitory starch metabolism in flag leaves of wheat after anthesis

2014 ◽  
Vol 153 (1) ◽  
pp. 12-29 ◽  
Author(s):  
Guozhang Kang ◽  
Xiaoqi Peng ◽  
Lina Wang ◽  
Yingying Yang ◽  
Ruixin Shao ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Ultrastructural Observation ◽  
Starch Metabolism ◽  
Temporal Expression ◽  
Mesophyll Cells ◽  
Flag Leaves ◽  
Transitory Starch

Temporal expression profiles of defense-related genes involved in Lactuca sativa- Sclerotinia sclerotiorum interactions

2021 ◽  
Author(s):  
Aqeel Hussein Abdulraoof Almatwari ◽  
Mohammadreza Hassandokht ◽  
Frouzandeh Soltani ◽  
Amir Mirzadi Gohari ◽  
Mohammad Javan-Nikkhah
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Lactuca Sativa ◽  
Expression Profiles ◽  
Temporal Expression

scholarly journals Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of the yellow-y Gene in Spodoptera litura

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiao-Long Liu ◽  
Wei-Kang Han ◽  
Long-Ji Ze ◽  
Ying-Chuan Peng ◽  
Yi-Lin Yang ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Expression Profiles ◽  
Marker Gene ◽  
The Body ◽  
Minor Role ◽  
Temporal Expression ◽  
Low Levels ◽  
Fat Bodies ◽  
Pupal Cuticle

Yellow genes are thought to be involved in the melanin biosynthetic pathway and play a crucial role in pigmentation reactions in insects. However, little research has been done on yellow genes in lepidopteran pests. To clarify the function of one of the yellow genes (yellow-y) in Spodoptera litura, we cloned the full-length of yellow-y, and investigated its spatial and temporal expression profiles by quantitative real-time PCR (qPCR). It revealed that yellow-y was highly expressed in larva of fourth, fifth, and sixth instars, as well as in epidermis (Ep), fat bodies (FB), Malpighian tubes (MT), and midguts (MG) of the larvae; whereas it was expressed in very low levels in different tissues of adults, and was almost undetected in pupa. This expression profile suggests an important role of yellow-y in larvae, minor role in adults, and no role in pupae. To confirm this, we disrupted yellow-y using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system, and obtained G0 insects with mutation in yellow-y. The mutation in yellow-y clearly rendered the larvae body, a color yellower than that of wide type insects, and in addition, the mutation resulted in abnormal segmentation and molting for older larvae. The mutation of yellow-y also made various adult tissues (antennae, proboscis, legs, and wings) yellowish. However, the mutation had no effect on pigmentation of the pupal cuticle. Taken together, our study clearly demonstrated the role of yellow-y not only in the body pigmentation of larvae and adults, and but also in segmentation and molting of larvae, providing new insights into the physiology of larval development, as well as a useful marker gene for genome editing based studies.

scholarly journals Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

2019 ◽  
Vol 27 (5) ◽  
pp. 998-1018 ◽  
Author(s):  
Olga Volovych ◽  
Zhe Lin ◽  
Jie Du ◽  
Hong Jiang ◽  
Zhen Zou
Keyword(s):  
Expression Profiles ◽  
Temporal Expression ◽  
Microplitis Mediator ◽  
Cuticular Proteins

scholarly journals Conditional clustering of temporal expression profiles

2008 ◽  
Vol 9 (1) ◽  
pp. 147 ◽  
Author(s):  
Ling Wang ◽  
Monty Montano ◽  
Matt Rarick ◽  
Paola Sebastiani
Keyword(s):  
Expression Profiles ◽  
Temporal Expression ◽  
Conditional Clustering

scholarly journals Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yichong Zhang ◽  
Yuanbo Zhan ◽  
Na Han ◽  
Yuhui Kou ◽  
Xiaofeng Yin ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Injury ◽  
Expression Profiles ◽  
Sciatic Nerve Injury ◽  
Temporal Expression

scholarly journals Tsn1-Mediated Host Responses to ToxA from Pyrenophora tritici-repentis

2009 ◽  
Vol 22 (9) ◽  
pp. 1056-1068 ◽  
Author(s):  
Tika B. Adhikari ◽  
Jianfa Bai ◽  
Steven W. Meinhardt ◽  
Suraj Gurung ◽  
Mary Myrfield ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Molecular Mechanisms ◽  
Wheat Cultivar ◽  
Expression Profiles ◽  
Phenylpropanoid Pathway ◽  
Host Responses ◽  
Mesophyll Cells ◽  
Pyrenophora Tritici Repentis ◽  
Host Defense Response

The toxin sensitivity gene Tsn1 interacts with Ptr ToxA (ToxA), a host-selective toxin produced by the necrotrophic fungus Pyrenophora tritici-repentis. The molecular mechanisms associated with cell death in sensitive wheat cultivars following ToxA application are not well understood. To address this question, we used the Affymetrix GeneChip Wheat Genome Array to compare gene expression in a sensitive wheat cultivar possessing the Tsn1 gene with the insensitive wheat cv. Nec103, which lacks the Tsn1 gene. This analysis was performed at early timepoints after infiltration with ToxA (e.g., 0.5 to 12 h postinfiltration [hpi]); at this time, ToxA is known to internalize into mesophyll cells without visible cell death symptoms. Gene expression also was monitored at later timepoints (24 to 48 hpi), when ToxA causes extensive damage in cellular compartments and visible cell death. At both early and late timepoints, numerous defense-related genes were induced (2- to 197-fold increases) and included genes involved in the phenylpropanoid pathway, lignification, and the production of reactive oxygen species (ROS). Furthermore, a subset of host genes functioning in signal transduction, metabolism, and as transcription factors was induced as a consequence of the Tsn1–ToxA interaction. Nine genes known to be involved in the host defense response and signaling pathways were selected for analysis by quantitative real-time polymerase chain reaction, and the expression profiles of these genes confirmed the results obtained in microarray experiments. Histochemical analyses of a sensitive wheat cultivar showed that H2O2 was present in leaves undergoing cell death, indicating that ROS signaling is a major event involved in ToxA-mediated cell death. The results suggest that recognition of ToxA via Tsn1 triggers transcriptional reprogramming events similar to those reported for avirulence–resistance gene interactions, and that host-derived genes play an important role in the modulation of susceptibility to P. tritici-repentis.

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