scholarly journals Head-group acylation of monogalactosyldiacylglycerol is a common stress response, and the acyl-galactose acyl composition varies with the plant species and applied stress

2013 ◽  
Vol 150 (4) ◽  
pp. 517-528 ◽  
Author(s):  
Hieu Sy Vu ◽  
Mary R. Roth ◽  
Pamela Tamura ◽  
Thilani Samarakoon ◽  
Sunitha Shiva ◽  
...  
Keyword(s):  
Stress Response ◽  
Plant Species ◽  
Applied Stress ◽  
Head Group ◽  
Acyl Composition

scholarly journals Leaf hydraulics and drought stress: response, recovery and survivorship in four woody temperate plant species

2009 ◽  
Vol 32 (11) ◽  
pp. 1584-1595 ◽  
Author(s):  
CHRISTOPHER J. BLACKMAN ◽  
TIMOTHY J. BRODRIBB ◽  
GREGORY J. JORDAN
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Plant Species ◽  
Leaf Hydraulics ◽  
Response Recovery

scholarly journals Genome-wide analysis of the apple CaCA superfamily reveals that MdCAX proteins are involved in the abiotic stress response as calcium transporters

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ke Mao ◽  
Jie Yang ◽  
Min Wang ◽  
Huayu Liu ◽  
Xin Guo ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Plant Species ◽  
Stress Responses ◽  
Calcium Transport ◽  
Sequence Similarity ◽  
Structural Basis ◽  
Functional Study ◽  
Transport Capacity ◽  
Abiotic Stress Response

Abstract Background Calcium (Ca2+) plays an important role in plant growth and development, and the maintenance of calcium homeostasis is necessary for the survival of all plant species. Ca2+/H+ exchangers (CAXs) are a subgroup of the CaCA (Ca2+/cation antiporter) superfamily. In general, CAX proteins mediate cytosolic Ca2+ entry into vacuoles to prevent excessive accumulation of Ca2+ in the cytosol. The CaCA superfamily has been identified and characterised in many plant species; however, characterisation of the CaCA superfamily and functional study of apple CAX proteins have yet to be conducted in apple (Malus × domestica Borkh.). Results Here, we identified 21 CaCA family proteins in apple for the first time. Phylogenetic and gene structure analysis, as well as prediction of conserved motifs, suggested that these proteins could be classified into four groups: CAX, CCX, NCL, and MHX. Expression analysis showed that the 10 MdCAX genes we cloned strongly responded to calcium and abiotic stress treatments. Collinearity analysis and characterisation of calcium transport capacity resulted in the identification of a pair of segmental duplication genes: MdCAX3L-1 and MdCAX3L-2; MdCAX3L-2 showed strong calcium transport capacity, whereas MdCAX3L-1 showed no calcium transport capacity. Yeast two-hybrid (Y2H) assays showed that these two proteins could interact with each other. The high sequence similarity (94.6%) makes them a good model for studying the crucial residues and structural basis of the calcium transport of CAX proteins. Prediction of the protein interaction network revealed several proteins that may interact with CAX proteins and play important roles in plant stress responses, such as SOS2, CXIP1, MHX, NRAMP3, and MTP8. Conclusions Our analysis indicated that MdCAX proteins have strong calcium transport capacity and are involved in the abiotic stress response in apple. These findings provide new insight and rich resources for future studies of MdCAX proteins in apple.

Plastic Deformation in Microtomed Sections

1971 ◽  
Vol 29 ◽  
pp. 458-459
Author(s):  
J. Temple Black
Keyword(s):  
Plastic Deformation ◽  
Plastic Strain ◽  
Applied Stress ◽  
Elastic Strain ◽  
High Strain ◽  
Tool Material ◽  
Cutting Edge ◽  
Material Structure ◽  
Geometrical Constraints

The output of the ultramicrotomy process with its high strain levels is dependent upon the input, ie., the nature of the material being machined. Apart from the geometrical constraints offered by the rake and clearance faces of the tool, each material is free to deform in whatever manner necessary to satisfy its material structure and interatomic constraints. Noncrystalline materials appear to survive the process undamaged when observed in the TEM. As has been demonstrated however microtomed plastics do in fact suffer damage to the top and bottom surfaces of the section regardless of the sharpness of the cutting edge or the tool material. The energy required to seperate the section from the block is not easily propogated through the section because the material is amorphous in nature and has no preferred crystalline planes upon which defects can move large distances to relieve the applied stress. Thus, the cutting stresses are supported elastically in the internal or bulk and plastically in the surfaces. The elastic strain can be recovered while the plastic strain is not reversible and will remain in the section after cutting is complete.

The effects of strain and strain rate on residual microstructures in copper

1986 ◽  
Vol 44 ◽  
pp. 420-421
Author(s):  
M. F. Stevens ◽  
P. S. Follansbee
Keyword(s):  
Strain Rate ◽  
Applied Stress ◽  
Threshold Stress ◽  
Rate Sensitivity ◽  
Viscous Drag ◽  
Strain Rates ◽  
Strain And Strain Rate ◽  
Threshold Strength ◽  
Mechanism Transition ◽  
Intrinsic Strength

The strain rate sensitivity of a variety of materials is known to increase rapidly at strain rates exceeding ∼103 sec-1. This transition has most often in the past been attributed to a transition from thermally activated guide to viscous drag control. An important condition for imposition of dislocation drag effects is that the applied stress, σ, must be on the order of or greater than the threshold stress, which is the flow stress at OK. From Fig. 1, it can be seen for OFE Cu that the ratio of the applied stress to threshold stress remains constant even at strain rates as high as 104 sec-1 suggesting that there is not a mechanism transition but that the intrinsic strength is increasing, since the threshold strength is a mechanical measure of intrinsic strength. These measurements were made at constant strain levels of 0.2, wnich is not a guarantee of constant microstructure. The increase in threshold stress at higher strain rates is a strong indication that the microstructural evolution is a function of strain rate and that the dependence becomes stronger at high strain rates.

A Transmission Electron Microscopical Investigation of Phase Transformations in TiNi

1980 ◽  
Vol 38 ◽  
pp. 340-341
Author(s):  
P. Moine ◽  
G. M. Michal ◽  
R. Sinclair
Keyword(s):  
Electron Microscopy ◽  
Phase Transformations ◽  
Applied Stress ◽  
Structural Changes ◽  
Microscopical Investigation ◽  
Temperature Range ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Diffraction Effects ◽  
Microscopy Images

Premartensitic effects in near equiatomic TiNi have been pointed out by several authors(1-5). These include anomalous contrast in electron microscopy images (mottling, striations, etc. ),diffraction effects(diffuse streaks, extra reflections, etc.), a resistivity peak above Ms (temperature at which a perceptible amount of martensite is formed without applied stress). However the structural changes occuring in this temperature range are not well understood. The purpose of this study is to clarify these phenomena.

CarR, a MarR-family regulator from Corynebacterium glutamicum, modulated antibiotic and aromatic compound resistance

2019 ◽  
Vol 476 (21) ◽  
pp. 3141-3159 ◽  
Author(s):  
Meiru Si ◽  
Can Chen ◽  
Zengfan Wei ◽  
Zhijin Gong ◽  
GuiZhi Li ◽  
...  
Keyword(s):  
Stress Response ◽  
Ligand Binding ◽  
Corynebacterium Glutamicum ◽  
Conformational Changes ◽  
Cysteine Oxidation ◽  
Transcriptional Regulatory ◽  
Ligand Free ◽  
Redox Sensing

Abstract MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this study, we characterized the stress-related ligand-binding functions of the C. glutamicum MarR-type regulator CarR (C. glutamicum antibiotic-responding regulator). We demonstrate that CarR negatively regulates the expression of the carR (ncgl2886)–uspA (ncgl2887) operon and the adjacent, oppositely oriented gene ncgl2885, encoding the hypothetical deacylase DecE. We also show that CarR directly activates transcription of the ncgl2882–ncgl2884 operon, encoding the peptidoglycan synthesis operon (PSO) located upstream of carR in the opposite orientation. The addition of stress-associated ligands such as penicillin and streptomycin induced carR, uspA, decE, and PSO expression in vivo, as well as attenuated binding of CarR to operator DNA in vitro. Importantly, stress response-induced up-regulation of carR, uspA, and PSO gene expression correlated with cell resistance to β-lactam antibiotics and aromatic compounds. Six highly conserved residues in CarR were found to strongly influence its ligand binding and transcriptional regulatory properties. Collectively, the results indicate that the ligand binding of CarR induces its dissociation from the carR–uspA promoter to derepress carR and uspA transcription. Ligand-free CarR also activates PSO expression, which in turn contributes to C. glutamicum stress resistance. The outcomes indicate that the stress response mechanism of CarR in C. glutamicum occurs via ligand-induced conformational changes to the protein, not via cysteine oxidation-based thiol modifications.

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