The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos

2013 ◽  
Vol 149 (2) ◽  
pp. 273-285 ◽  
Author(s):  
Edward Businge ◽  
Joakim Bygdell ◽  
Gunnar Wingsle ◽  
Thomas Moritz ◽  
Ulrika Egertsdotter
Keyword(s):  
Norway Spruce ◽  
Somatic Embryos ◽  
Reserve Accumulation ◽  
Storage Reserve

Developmental profile of storage reserve accumulation in soybean somatic embryos

2011 ◽  
Vol 47 (6) ◽  
pp. 725-733 ◽  
Author(s):  
Yan He ◽  
Tammie E. Young ◽  
Karen Rebecca Clark ◽  
Kathryn F. Kleppinger-Sparace ◽  
William C. Bridges ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Developmental Profile ◽  
Reserve Accumulation ◽  
Storage Reserve

scholarly journals Abscisic Acid and High Osmoticum Regulation of Development and Storage Reserve Accumulation in Sugarcane Somatic Embryos.

1994 ◽  
Vol 63 (4) ◽  
pp. 689-698 ◽  
Author(s):  
Ebiamadon Andi BRISIBE ◽  
Hiroshi MIYAKE ◽  
Takeshi TANIGUCHI ◽  
Eizo MAEDA
Keyword(s):  
Abscisic Acid ◽  
Somatic Embryos ◽  
Reserve Accumulation ◽  
Storage Reserve ◽  
And Storage

scholarly journals Nitrogen utilization during germination of somatic embryos of Norway spruce: revealing the importance of supplied glutamine for nitrogen metabolism

Trees ◽  
2018 ◽  
Vol 33 (2) ◽  
pp. 383-394 ◽  
Author(s):  
Johanna Carlsson ◽  
Ulrika Egertsdotter ◽  
Ulrika Ganeteg ◽  
Henrik Svennerstam
Keyword(s):  
Nitrogen Metabolism ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Nitrogen Utilization

scholarly journals Storage Reserve Accumulation in Arabidopsis: Metabolic and Developmental Control of Seed Filling

2008 ◽  
Vol 6 ◽  
pp. e0113 ◽  
Author(s):  
Sébastien Baud ◽  
Bertrand Dubreucq ◽  
Martine Miquel ◽  
Christine Rochat ◽  
Loïc Lepiniec
Keyword(s):  
Developmental Control ◽  
Seed Filling ◽  
Reserve Accumulation ◽  
Storage Reserve

Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

Micron ◽  
2016 ◽  
Vol 84 ◽  
pp. 67-71 ◽  
Author(s):  
Vilém Neděla ◽  
Jiří Hřib ◽  
Ladislav Havel ◽  
Jiří Hudec ◽  
Jiří Runštuk
Keyword(s):  
Norway Spruce ◽  
Somatic Embryos ◽  
Laser Scanning ◽  
Scanning Microscope ◽  
Laser Scanning Microscope

scholarly journals Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Jindřich Bříza ◽  
Daniela Pavingerová ◽  
Josef Vlasák ◽  
Hana Niedermeierová
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Bacillus Thuringiensis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Marker Gene ◽  
Selection Marker ◽  
Embryogenic Tissue ◽  
35S Promoter ◽  
Rt Pcr

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

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