Artichoke yellow ringspot virus as the causal agent of a new viral disease of lettuce: Epidemiology and molecular variability

2020 ◽  
Author(s):  
Lefkothea Karapetsi ◽  
Elisavet K. Chatzivassiliou ◽  
Nikolaos I. Katis ◽  
Varvara I. Maliogka
Keyword(s):  
Viral Disease ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Molecular Variability

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

scholarly journals Yellow Net of Triumffeta Is Caused by a Geminivirus: A First Report

Plant Disease ◽  
1998 ◽  
Vol 82 (1) ◽  
pp. 127-127 ◽  
Author(s):  
Vipin Hallan ◽  
Sangeeta Saxena ◽  
B. P. Singh
Keyword(s):  
Rainy Season ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Viral Disease ◽  
Hybridization Experiment ◽  
Dorsal Side ◽  
Causal Agent ◽  
Degenerate Primers ◽  
First Report ◽  
Initial Symptoms

Triumffeta rhomboidiaceae Jacq. (Tiliaceae family) is an annual rainy season weed that is commonly found throughout India. For the last 3 years, during the rainy season, several plants of T. rhomboidiaceae in and around the gardens of the National Botanical Research Institute have been found with vein yellowing symptoms. The initial symptoms were vein clearing but in later stages the veins became yellow and thickened. In severe cases, the chlorosis extends into interveinal areas, resulting in complete yellowing of the leaves. In a few cases, green leafy or thorny enations could be seen on the dorsal side of the leaf. The disease was investigated to identify the causal agent. Vector transmission studies showed that the causal agent is transmitted by the whitefly, Bemisia tabaci, from infected to healthy seedlings of T. rhomdoidiaceae. Since whitefly transmission of the disease is consistent with a geminivirus as the causal agent, the role of such a virus was investigated. DNA isolated from Triumffeta plants (both from the infected plants in the field as well as from those inoculated experimentally in the greenhouse) showing above mentioned symptoms was amplified with two sets of degenerate primers, PAL1v1978/PAR1c496 (set 1) and PAL1v1978/PCRc1 (set 2), that have been shown to be specific for DNA-A of whitefly transmitted geminiviruses (WTGs), in polymerase chain reaction (1). We could amplify DNA-A fragments of approximately 1.2 kb from set 1 and 0.7 kb from set 2, as expected (1). DNA isolated from healthy seedlings gave no amplification of such fragments. Identification of the amplified DNA fragments (from infected samples) to be of geminiviral in nature was confirmed by Southern blot hybridization carried out under high stringency conditions. DNA-A of Indian tomato leaf curl virus (2) was used as a general probe for WTGs for the above hybridization experiment. Therefore, Triumffeta yellow net disease is caused by a geminivirus. A review of literature revealed that there is no record of a viral disease affecting this weed and, therefore, this is the first report of a viral disease affecting this plant. References: (1) M. R. Rojas et al. Plant Dis. 77:340, 1993. (2) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995.

scholarly journals First Report of Papaya ringspot virus and Zucchini yellow mosaic virus in Luohanguo (Siraitia grosvenorii) in China

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 530-530 ◽  
Author(s):  
Y.-M. Liao ◽  
X.-J. Gan ◽  
B. Chen ◽  
J.-H. Cai
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Viral Disease ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
First Report ◽  
Siraitia Grosvenorii

Luohanguo, Siraitia grosvenorii (Swingle) C. Jeffrey, is a perennial cucurbitaceous plant that is an economically important medicinal and sweetener crop in Guangxi province, China. Surveys conducted during the summer to fall seasons of 2003-2004 in northern Guangxi showed symptoms typical of a viral disease, including leaf mottling, mosaic, vein clearing, curling, and shoestring-like distortion in the field. Mechanical inoculation of sap from leaves of symptomatic plants collected from the surveyed areas caused similar symptoms on tissue culture-derived healthy Luohanguo plants. Two sequences of 0.7 and 1.6 kb with 88 and 97% identity to Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) were amplified using reverse transcription-polymerase chain reaction (RT-PCR) with purified flexuous viral particles or total RNA extracted from the symptomatic Luohanguo leaves as templates with conserved degenerate potyvirus primers (1). To confirm the results, primers specific for PRSV (PP1/PP2, genome coordinates 4064-4083/5087-5069, GenBank Accession No X97251) and ZYMV (ZP1/ZP2, genome coordinates 5540-5557/7937-7920, GenBank Accession No L31350) were used to perform RT-PCR from the same RNA templates. The expected 1.0- and 2.3-kb fragments were amplified and they were 90 and 95% identical to PRSV and ZYMV in sequence, respectively. Watermelon mosaic virus was not detected. To our knowledge, this is the first report of the occurrence of PRSV and ZYMV in Luohanguo. Reference: (1) A. Gibbs et al. J. Virol. Methods 63:9, 1997.

Studies on molecular variability of coat protein gene of Papaya ringspot virus-P isolates from India

2019 ◽  
Vol 155 (1) ◽  
pp. 369-376 ◽  
Author(s):  
Rajesh Kumar ◽  
Ashish Srivastava ◽  
Aparana Srivastava ◽  
Shalini Srivastava ◽  
Vivek Prasad ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Molecular Variability

scholarly journals Citrus Diseases Exotic to Florida: Citrus Leprosis

EDIS ◽  
2020 ◽  
Vol 2020 (5) ◽  
Author(s):  
Ozgur Batuman ◽  
Amit Levy ◽  
Ajia Paolillo ◽  
Kuang-Ren Chung ◽  
Ron Brlansky
Keyword(s):  
Plant Pathology ◽  
Viral Disease ◽  
Causal Agent ◽  
Citrus Industry ◽  
Previous Version ◽  
Major Revision ◽  
Citrus Diseases ◽  
Citrus Leprosis ◽  
Florida Citrus

This article is one in a series designed to provide important information on the causal agent, symptoms, and transmission of exotic citrus diseases. Disseminating the information about the diseases to the citrus industry may prevent their introduction and spread in Florida. This 5-page document will focus on the exotic viral disease citrus leprosis. This is a major revision of an article originally published in 2006. Written by O. Batuman, A. Levy, P. Sieburth, A. M. Paolillo, K.-R. Chung, and R. H. Brlansky, and published by the UF/IFAS Plant Pathology Department.https://edis.ifas.ufl.edu/pp148 Previous version: Chung, Kuang-Ren, and Ronald Brlansky. 2006. “Citrus Diseases Exotic to Florida: Citrus Leprosis”. EDIS 2006 (7). https://journals.flvc.org/edis/article/view/115752.

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