Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia

2020 ◽  
Vol 69 (6) ◽  
pp. 1026-1033 ◽  
Author(s):  
Aamir Lal ◽  
Eui‐Joon Kil ◽  
Vo T. B. Thuy ◽  
Chairina Fadhila ◽  
Phuong T. Ho ◽  
...  
2005 ◽  
Vol 86 (6) ◽  
pp. 1851-1860 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Shigemi Seo ◽  
...  

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.


Crop Science ◽  
1999 ◽  
Vol 39 (1) ◽  
pp. 158-163 ◽  
Author(s):  
Walter E. Riedell ◽  
Robert W. Kieckhefer ◽  
Scott D. Haley ◽  
Marie A. C Langham ◽  
Paul D. Evenson

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 344 ◽  
Author(s):  
Tong Zhang ◽  
Wendi Feng ◽  
Jiajie Ye ◽  
Zhanbiao Li ◽  
Guohui Zhou

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