Multilocus identification and thiophanate‐methyl sensitivity of Colletotrichum gloeosporioides species complex associated with fruit with symptoms and symptomless leaves of mango

2020 ◽  
Vol 69 (6) ◽  
pp. 1125-1138 ◽  
Author(s):  
Dionicio Fuentes‐Aragón ◽  
Vladimiro Guarnaccia ◽  
Angel Rebollar‐Alviter ◽  
Sandra B. Juárez‐Vázquez ◽  
Fortino Aguirre‐Rayo ◽  
...  
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Thiophanate Methyl ◽  
Colletotrichum Gloeosporioides Species Complex

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

scholarly journals Identification of the Colletotrichum species associated with mango diseases and a universal LAMP detection method for C. gloeosporioides species complex

2021 ◽  
pp. 49-60
Author(s):  
Yukako Hattori ◽  
Chiharu Nakashima ◽  
Shunsuke Kitabata ◽  
Kosuke Naito ◽  
Ayaka Hieno ◽  
...  
Keyword(s):  
Species Complex ◽  
Plant Disease ◽  
Plant Pathogens ◽  
Colletotrichum Gloeosporioides ◽  
Detection Method ◽  
Coding Region ◽  
Gene Coding ◽  
Detectable Range ◽  
Amplification Assay ◽  
Colletotrichum Gloeosporioides Species Complex

Abstract: The Colletotrichum gloeosporioides species complex contains plant pathogens linked to Anthracnose diseases afflicting various crops. In this study, we designed a loop-mediated isothermal amplification assay (LAMP) primer set based on calmodulin gene coding region sequences from taxonomically authorized isolates of species from this complex to rapidly detect the presence of fungi associated with Anthracnose diseases. This test can be employed at any point between cultivation and sale. Moreover, we examined the specificity and detectable range of this primer set using isolates selected from species of the genus Colletotrichum. This test was able to specifically detect members of the C. gloeosporioides species complex, including C. gloeosporioides, C. aotearoa, C. fructicola, C. horii, C. kahawae, C. musae, C. siamense, C. theobromicola, and C. tropicale. Key Words: Anthracnose, diagnosis, phylogeny, plant disease

scholarly journals First Report of Anthracnose Caused by Colletotrichum gloeosporioides on Schefflera actinophylla in China

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 998-998
Author(s):  
J. Huang
Keyword(s):  
Dna Sequence ◽  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Conidial Suspension ◽  
First Report ◽  
Leaf Spots ◽  
Black Spots ◽  
Pathogenicity Tests ◽  
Colletotrichum Gloeosporioides Species Complex ◽  
And Control

In China, in mild to warm climates, Schefflera actinophylla is commonly grown as a decorative tree in gardens. When mature, it has bright red flowers in inflorescences with up to 20 racemes that develop in summer or early autumn. From 2008 to 2011, lesions were observed on young and mature leaves in several locations in Guangzhou, China. The first symptoms were circular, necrotic areas that usually developed into irregular, dry, brown to reddish brown or black spots. Spots often first appeared at or near the margins of leaves. Reproductive bodies of the pathogen appeared as black specks in leaf spots. Under a 10× magnification, black, needle-like fungal structures (setae) were observed in the centers of spots on the upper leaf surface. A fungus was isolated from the lesion and was identified as Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. based on cultural characteristics and conidial morphology (1). The voucher isolates were deposited in the Institute of Plant Pathology, Zhongkai University of Agriculture and Engineering. C. gloeosporioides is a species complex (2) and there is a degree of unresolved aspects of taxonomy in this species complex. Cultures on potato dextrose agar (PDA) had aerial white mycelium that turned gray to grayish black after 10 days at 25°C and a 12-h photoperiod and produced salmon to orange conidial masses. Brown, 80 to 120 μm long setae were observed in the acervulus. Conidia 14.1 to 18.0 × 4.0 to 6.1 μm in size were hyaline, thin-walled, aseptate, granular inside, clavate to slightly navicular in shape with an obtuse apex and a truncate base. To identify the fungus, a 588-bp segment of the ITS1-5.8S-ITS2 rDNA region was amplified by PCR and sequenced. The DNA sequence was submitted to GenBank as KC207404. A BLAST search of the DNA sequence showed 99% identity with accessions AY266389.1, EF423519.1, and HM575258.1 of C. gloeosporioides. Pathogenicity tests were conducted under greenhouse conditions at 25 ± 2°C. A total of 15 leaves from three 1-year-old S. actinophylla plants were inoculated with mycelial PDA plugs that were placed on 0.5-cm2 leaf wounds and then wrapped with Parafilm. Control leaves were treated similarly except that they were inoculated with PDA plugs without the fungus. No symptoms developed on control leaves after 10 days. Foliar lesions on inoculated leaves closely resembled those observed in the field. C. gloeosporioides was reisolated consistently from inoculated leaves. Pathogenicity was also tested by spraying leaves of potted S. actinophylla plants about 30 cm in height with 10 ml of a conidial suspension (1 × 105 conidia/ml) prepared from 7-day-old PDA cultures grown at 25°C. Leaves sprayed with distilled water were used as controls. Three plants were inoculated in each of two experiments and were incubated at 25°C and 90% relative humidity in a growth chamber. Tiny brown spots started to develop on all inoculated leaves 5 days after inoculation and the progression of symptom development was similar to that observed in the field. Control leaves remained asymptomatic. C. gloeosporioides was reisolated from inoculated leaves. To my knowledge, this is the first report of C. gloeosporioides causing anthracnose on S. actinophylla in China. References: (1) B. C. Sutton. The genus Glomerella and its anamorph Colletotrichum. In: Colletotrichum Biology, Pathology and Control. CAB International, Wallingford, UK, 1992. (2) B. S. Weir et al. The Colletotrichum gloeosporioides species complex. Stud. Mycol. 73:115, 2012.

Sensitivity to MBC fungicides and prochloraz of Colletotrichum gloeosporioides species complex isolates from mango orchards in Mexico

2021 ◽  
Author(s):  
José A. Mora-Aguilera ◽  
Erendira G. Ríos-López ◽  
Martín Yáñez-Zúñiga ◽  
Angel Rebollar-Alviter ◽  
Cristian Nava-Díaz ◽  
...  
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Gloeosporioides Species Complex

scholarly journals The etiological agent of cotton ramulosis represents a single phylogenetic lineage within the Colletotrichum gloeosporioides species complex

2014 ◽  
Vol 39 (5) ◽  
pp. 357-367 ◽  
Author(s):  
Maria Eloisa Salustiano ◽  
Marina Nunes Rondon ◽  
Lucas M. Abreu ◽  
Sarah da Silva Costa ◽  
José da Cruz Machado ◽  
...  
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Etiological Agent ◽  
Phylogenetic Lineage ◽  
Colletotrichum Gloeosporioides Species Complex
Sign in / Sign up

Export Citation Format

Share Document