Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature‐sensitive manner

2019 ◽  
Vol 68 (5) ◽  
pp. 1025-1032
Author(s):  
T. Hamada ◽  
K. Mise ◽  
A. Kiba ◽  
Y. Hikichi
Keyword(s):  
Mosaic Virus ◽  
Temperature Sensitive ◽  
Japanese Isolate ◽  
Systemic Necrosis

scholarly journals Cell Death Triggered by a Putative Amphipathic Helix of Radish mosaic virus Helicase Protein Is Tightly Correlated With Host Membrane Modification

2015 ◽  
Vol 28 (6) ◽  
pp. 675-688 ◽  
Author(s):  
Masayoshi Hashimoto ◽  
Ken Komatsu ◽  
Ryo Iwai ◽  
Takuya Keima ◽  
Kensaku Maejima ◽  
...  
Keyword(s):  
Cell Death ◽  
Mosaic Virus ◽  
Defense Responses ◽  
Plant Viruses ◽  
Amphipathic Helix ◽  
Membrane Structures ◽  
Membrane Modification ◽  
Systemic Necrosis ◽  
Amino Terminal ◽  
Er Membrane

Systemic necrosis is one of the most severe symptoms caused by plant RNA viruses. Recently, systemic necrosis has been suggested to have similar features to a defense response referred to as the hypersensitive response (HR), a form of programmed cell death. In virus-infected plant cells, host intracellular membrane structures are changed dramatically for more efficient viral replication. However, little is known about whether this replication-associated membrane modification is the cause of the symptoms. In this study, we identified an amino-terminal amphipathic helix of the helicase encoded by Radish mosaic virus (RaMV) (genus Comovirus) as an elicitor of cell death in RaMV-infected plants. Cell death caused by the amphipathic helix had features similar to HR, such as SGT1-dependence. Mutational analyses and inhibitor assays using cerulenin demonstrated that the amphipathic helix–induced cell death was tightly correlated with dramatic alterations in endoplasmic reticulum (ER) membrane structures. Furthermore, the cell death–inducing activity of the amphipathic helix was conserved in Cowpea mosaic virus (genus Comovirus) and Tobacco ringspot virus (genus Nepovirus), both of which are classified in the family Secoviridae. Together, these results indicate that ER membrane modification associated with viral intracellular replication may be recognized to prime defense responses against plant viruses.

Alfalfa mosaic virus temperature-sensitive mutants III. Mutants with a putative defect in cell-to-cell transport

Virology ◽  
1986 ◽  
Vol 154 (2) ◽  
pp. 401-404 ◽  
Author(s):  
Marianne J. Huisman ◽  
Alberto N. Sarachu ◽  
Fieke Ablas ◽  
Helgo J.G. Broxterman ◽  
Lous Van Vloten-Doting ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Temperature Sensitive ◽  
Cell Transport ◽  
Temperature Sensitive Mutants

scholarly journals Sequence Variations Among 17 New Radish Isolates of Turnip mosaic virus Showing Differential Pathogenicity and Infectivity in Nicotiana benthamiana, Brassica rapa, and Raphanus sativus

2019 ◽  
Vol 109 (5) ◽  
pp. 904-912 ◽  
Author(s):  
Junsu Gong ◽  
Hye-Kyoung Ju ◽  
Ik-Hyun Kim ◽  
Eun-Young Seo ◽  
In-Sook Cho ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Chinese Cabbage ◽  
Nicotiana Benthamiana ◽  
Turnip Mosaic Virus ◽  
Amino Acid Residues ◽  
Systemic Necrosis ◽  
Infectious Clones ◽  
Sequence Variations ◽  
Distinct Sequence

Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV.

Alfalfa mosaic virus temperature-sensitive mutants

Virology ◽  
1989 ◽  
Vol 171 (2) ◽  
pp. 409-416 ◽  
Author(s):  
Marianne J. Huisman ◽  
Ben J.C. Cornelissen ◽  
Carolina F.M. Groenendijk ◽  
John F. Bol ◽  
Lous Van Vloten-Doting
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

scholarly journals New Sources of Temperature-Sensitive Resistance to Wheat streak mosaic virus in Wheat

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1051-1056 ◽  
Author(s):  
Dallas L. Seifers ◽  
Steve Haber ◽  
T. J. Martin ◽  
Guorong Zhang
Keyword(s):  
Mosaic Virus ◽  
Relative Effectiveness ◽  
Wheat Streak Mosaic Virus ◽  
Temperature Sensitive ◽  
Yield Losses ◽  
Sources Of Resistance ◽  
Small Grains ◽  
Virus Isolates ◽  
Better Than

Expressing temperature-sensitive resistance (TSR) protects wheat against yield losses from infection with Wheat streak mosaic virus (WSMV). In examining how 2,429 wheat accessions from the National Small Grains Collection responded to inoculation with the Sid81 isolate of WSMV, 20 candidate TSR sources were discovered. To differentiate their relative effectiveness, accession responses over 21 days to inoculation with GH95, Sid81, and PV57 virus isolates in regimes of 18 and 20°C were observed. At 18°C, all 20 candidate TSR sources were uniformly or nearly uniformly asymptomatic 21 days after inoculation with the PV57 isolate, resistance indistinguishable from resistant checks KS96HW10-3 and RonL. By contrast, the Sid81 isolate induced symptoms in low but significant proportions of plants of two candidates, and the GH95 isolate in high proportions for four candidates and low but significant proportions for two others. In the more stringent 20°C regime, the uniform or near-uniform induction of symptoms in response to inoculation with GH95 failed to differentiate among the 20 candidate TSR sources and two resistant checks, while PV57 and Sid81 identified several candidates that performed similarly to KS96HW10-3 and significantly better than RonL. By identifying new sources of resistance, this study contributes to the control of WSMV.

Alfalfa mosaic virus temperature-sensitive mutants IV. Tbts 7, a coat protein mutant defective in an early function

Virology ◽  
1987 ◽  
Vol 160 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Marianne J. Huisman ◽  
Frank C. Lanfermeyer ◽  
L. Sue Loesch-Fries ◽  
Lous Van Vloten-Doting ◽  
John F. Bol
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Early Function
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