Contrasting canopy and fibrous root damage on Swingle citrumelo caused by ‘Candidatus Liberibacter asiaticus’ and Phytophthora nicotianae

2017 ◽  
Vol 67 (1) ◽  
pp. 202-209 ◽  
Author(s):  
J. Wu ◽  
E. G. Johnson ◽  
K. M. Gerberich ◽  
D. B. Bright ◽  
J. H. Graham
Keyword(s):  
Phytophthora Nicotianae ◽  
Root Damage ◽  
Candidatus Liberibacter Asiaticus ◽  
Fibrous Root ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Swingle Citrumelo

scholarly journals Comportamiento de la lima 'Tahití’ (Citrus latifolia Tan) injertada en cinco patrones en dos zonas productoras de Puerto Rico

2017 ◽  
Vol 101 (2) ◽  
pp. 225-236
Author(s):  
Félix M. Román-Pérez ◽  
Agenol González-Vélez ◽  
Raúl Macchiavelli ◽  
Consuelo Estévez de Jensen
Keyword(s):  
Puerto Rico ◽  
Citrus Paradisi ◽  
Candidatus Liberibacter Asiaticus ◽  
Citrus Latifolia ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Swingle Citrumelo

La lima 'Tahiti', Citrus latifolia Tan. injertada en cinco patrones de cítricas: 'Swingle citrumelo' [Citrus paradisi Macf. x Ponsirus trifoliata (L.) Raf.], 'Carrizo' [C. sinensis x P. trifoliata (L.) Raf.] , 'HRS 812' [mandarina 'Sunki', C. reticulata x P. trifoliata (L.) Raf.], 'Limón rugoso' [C. jambhiri] y mandarina 'Cleopatra' [Citrus reshni Hort. Ex Tan.] se evaluó en las subestaciones de Isabela y Corozal, Puerto Rico. Los parámetros de altura, diámetro de copa, volumen de copa, eficiencia, número y peso de frutas por árbol, porcentaje de jugo, sólidos solubles totales, acidez y relación sólidos solubles totales/acidez (índice de madurez) fueron evaluados. En la Subestación de Isabela los mayores rendimientos se consiguieron en los patrones 'Limón rugoso' y 'Swingle', con 42.7 y 35.9 kg de fruta por árbol, respectivamente. Estos valores no difieren significativamente de los rendimientos conseguidos en los patrones 'HRS 812' (23.6 kg) y 'Cleopatra' (28.7 kg), mientras que superan significativamente los rendimientos en el patrón 'Carrizo' (21.6 kg). En la Subestación de Corozal los mayores rendimientos se consiguieron en los patrones 'Limón rugoso' (52.9 kg), 'HRS 812' (52.7 kg) y 'Swingle' (47.6 kg), siendo estos significativamente mayores que el de 'Cleopatra' (27.7 kg) pero no que el de 'Carrizo' (40.9 kg). En ambas localidades los parámetros que definen la calidad de fruta fueron aceptables y muy parecidos para ambas zonas. Dos años después de la siembra, en los árboles en Isabela se detectó a Candidatus Liberibacter asiaticus, bacteria asociada a la enfermedad conocida como "Huanglongbing" (HLB). A pesar de la presencia de la enfermedad, los árboles se mantuvieron en producción y no hubo declinamiento que se asociara a la enfermedad. Tras la detección de la enfermedad en esta localidad se estableció un programa nutricional más intensivo utilizado fertilizantes foliares y al suelo.

scholarly journals Two ‘Candidatus Liberibacter asiaticus’ Strains Recently Found in California Harbor Different Prophages

2017 ◽  
Vol 107 (6) ◽  
pp. 662-668 ◽  
Author(s):  
Z. Zheng ◽  
F. Wu ◽  
L. B. Kumagai ◽  
M. Polek ◽  
X. Deng ◽  
...  
Keyword(s):  
Los Angeles ◽  
Geographic Region ◽  
Mapping Technique ◽  
Polymorphism Analysis ◽  
Candidatus Liberibacter Asiaticus ◽  
Next Generation Sequencing Technology ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

‘Candidatus Liberibacter asiaticus’ (CLas), an α-proteobacterium, is associated with citrus Huanglongbing (HLB; yellow shoot disease). In California, two cases of CLas have been detected in Los Angeles County, one in Hacienda Heights in 2012 and the other in San Gabriel in 2015. Although all infected trees were destroyed in compliance with a state mandate, citrus industry stakeholder concerns about HLB in California are high. Little is known about the biology of CLas, particularly the California strains, hindering effective HLB management efforts. In this study, next-generation sequencing technology (Illumina MiSeq) was employed to characterize the California CLas strains. Data sets containing >4 billion (Giga) bp of sequence were generated from each CLas sample. Two prophages (P-HHCA1-2 and P-SGCA5-1) were identified by the MiSeq read mapping technique referenced to two known Florida CLas prophage sequences, SC1 and SC2. P-HHCA1-2 was an SC2-like or Type 2 prophage of 38,989 bp in size. P-SGCA5-1 was an SC1-like or Type 1 prophage of 37,487 bp in size. Phylogenetic analysis revealed that P-HHCA1-2 was part of an Asiatic lineage within the Type 2 prophage group. Similarly, P-SGCA5-1 was part of an Asiatic lineage within Type 1 prophage group. The Asiatic relatedness of both P-HHCA1-2 and P-SGCA5-1 was further presented by single nucleotide polymorphism analysis at terL (encoding prophage terminase) that has been established for CLas strain differentiation. The presence of different prophages suggests that the two California CLas strains could have been introduced from different sources. An alternative explanation is that there was a mixed CLas population containing the two types of prophages, and limited sampling in a geographic region may not accurately depict the true CLas diversity. More accurate pathway analysis may be achieved by including more strains collected from the regions.

scholarly journals Susceptibility of Sixteen Citrus Genotypes to ‘Candidatus Liberibacter asiaticus’

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1080-1086 ◽  
Author(s):  
Greg McCollum ◽  
Mark Hilf ◽  
Mike Irey ◽  
Weiqi Luo ◽  
Tim Gottwald
Keyword(s):  
The United States ◽  
Insect Vector ◽  
Candidatus Liberibacter Asiaticus ◽  
Citrus Germplasm ◽  
Citrus Production ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Florida Citrus ◽  
Citrus Macrophylla ◽  
Free Ranging

Huanglongbing (HLB) disease is the most serious threat to citrus production worldwide and, in the last decade, has devastated the Florida citrus industry. In the United States, HLB is associated with the phloem-limited α-proteobacterium ‘Candidatus Liberibacter asiaticus’ and its insect vector, the Asian citrus psyllid (ACP; Diaphorina citri). Significant effort is being put forth to develop novel citrus germplasm that has a lower propensity to succumb to HLB than do currently available varieties. Effective methods of screening citrus germplasm for susceptibility to HLB are essential. In this study, we exposed small, grafted trees of 16 citrus types to free-ranging ACP vectors and ‘Ca. L. asiaticus’ inoculum in the greenhouse. During 45 weeks of exposure to ACP, the cumulative incidence of ‘Ca. L. asiaticus’ infection was 70%. Trees of Citrus macrophylla and C. medica were most susceptible to ‘Ca. L. asiaticus’, with 100% infection by the end of the test period in three trials, while the complex genetic hybrids ‘US 1-4-59’ and ‘Fallglo’ consistently were least susceptible, with approximately 30% infection. Results obtained in this greenhouse experiment showed good agreement with trends observed in the orchard, supporting the validity of our approach for screening citrus germplasm for susceptibility to HLB.

scholarly journals A New Diagnostic System for Detection of ‘Candidatus Liberibacter asiaticus’ Infection in Citrus

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1295-1300 ◽  
Author(s):  
Lianming Lu ◽  
Baoping Cheng ◽  
Jinai Yao ◽  
Aitian Peng ◽  
Danchao Du ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Polyclonal Antibodies ◽  
Diagnostic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Candidatus Liberibacter Asiaticus ◽  
Negative Results ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Polymerase Chain

In this study, two polyclonal antibodies were produced against the Omp protein of ‘Candidatus Liberibacter asiaticus’. First, omp genes were sequenced to exhibit 99.9% identity among 137 isolates collected from different geographical origins. Then, two peptides containing the hydrophobic polypeptide-transport-associated (POTRA) domain and β-barrel domain, respectively, were identified on Omp protein. After that, these two peptides were overexpressed in Escherichia coli and purified by affinity chromatography to immunize the white rabbits. Finally, the antiserum was purified by affinity chromatography. The two Omp antibodies gave positive results (0.454 to 0.633, 1:1,600 dilution) in enzyme-linked immunosorbent assay against ‘Ca. L. asiaticus’-infected samples collected from different geographical origins but revealed negative results against other pathogen-infected, nutrient-deficient and healthy samples. The antibody against the POTRA domain of Omp protein could detect ‘Ca. L. asiaticus’ in 45.7% of the symptomatic samples compared with a 56.2% detection rate with a polymerase chain reaction assay. These new antibodies will provide a very useful supplement to the current approaches to ‘Ca. L. asiaticus’ detection and also provide powerful research tools for tracking distribution of this pathogen in vivo.

scholarly journals First report of ‘Candidatus Liberibacter asiaticus’ in Diaphorina communis

2011 ◽  
Vol 7 (1) ◽  
pp. 1-4 ◽  
Author(s):  
N. J. Donovan ◽  
G. A. C. Beattie ◽  
G. A. Chambers ◽  
P. Holford ◽  
A. Englezou ◽  
...  
Keyword(s):  
Candidatus Liberibacter Asiaticus ◽  
First Report ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus
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