scholarly journals Development of a lateral flow device for in-field detection and evaluation of PCR-based diagnostic methods forXanthomonas campestrispv.musacearum, the causal agent of banana xanthomonas wilt

2014 ◽  
Vol 64 (3) ◽  
pp. 559-567 ◽  
Author(s):  
J. Hodgetts ◽  
G. Karamura ◽  
G. Johnson ◽  
J. Hall ◽  
K. Perkins ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Causal Agent ◽  
Diagnostic Methods ◽  
Field Detection ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Banana Xanthomonas Wilt

Evaluation of rapid test kits for quantification of HT-2 and T-2 toxins in naturally contaminated oats

2013 ◽  
Vol 6 (1) ◽  
pp. 31-41 ◽  
Author(s):  
H.U. Aamot ◽  
I.S. Hofgaard ◽  
G. Brodal ◽  
O. Elen ◽  
B. Holen ◽  
...  
Keyword(s):  
Concentration Ratio ◽  
Rapid Test ◽  
Reference Method ◽  
Lateral Flow ◽  
Cross Reaction ◽  
Cross Reactions ◽  
Lateral Flow Device ◽  
Simultaneous Testing ◽  
Flow Device ◽  
Mycotoxin Analysis

The aim of this study was to evaluate the performance and usefulness of three rapid test kits for analysis of HT-2 and T-2 toxins (HT-2 and T-2), two of the most potent trichothecenes commonly found in European oats. Concentrations of these two toxins combined (HT-2+T-2) were analysed in naturally contaminated oat samples (n=68) using the following test kits: Ridascreen® FAST T-2 Toxin (‘Fast ELISA’), DRAFT Ridascreen® HT-2/T-2 (‘Standard ELISA’, not commercially available), and the lateral flow device ROSA® HT-2-T-2 (‘Rosa LFD’). Mycotoxin analysis by LC-MS/MS was used as a reference method. Rosa LFD offered the best reliability, achieving detection that was stable across toxin levels, whereas detection by both ELISA kits differed significantly among toxin levels (P<0.01). The kits were also evaluated regarding agreement with the reference method (measured as Cohen's kappa) at a HT-2+T-2 concentration of 1000 μg/kg in naturally contaminated oats. Agreement was greatest for Rosa LFD (89.2%), intermediate for Standard ELISA (66.8%), and lowest for Fast ELISA (62.2%). Rosa LFD showed cross-reaction of 100% with both T-2 and HT-2. For the ELISA kits, cross-reactions were 100% with T-2 but below 100% with HT-2. Therefore, to estimate the sum of HT-2 and T-2 in an oat sample, it was necessary to re-calculate the data from both ELISA kits according to the known cross-reaction of each kit with HT-2 and the concentration ratio of HT-2 to T-2 in Norwegian oats. Rosa LFD had the highest correlation with LC-MS/MS (R2=0.94), and the corresponding R2 values for Fast and Standard ELISA were 0.61 and 0.83, respectively. Rosa LFD was well suited for on-site detection. Standard ELISA allows simultaneous testing of several samples that are useful for centralised laboratories.

scholarly journals Engineering a simple lateral flow device for animal blood coagulation monitoring

2018 ◽  
Vol 12 (1) ◽  
pp. 014110 ◽  
Author(s):  
Hua Li ◽  
Daewoo Han ◽  
Giovanni M. Pauletti ◽  
Andrew J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Lateral Flow ◽  
Animal Blood ◽  
Lateral Flow Device ◽  
Coagulation Monitoring ◽  
Flow Device

scholarly journals A point-of-care neutrophil elastase activity assay identifies bronchiectasis severity, airway infection and risk of exacerbation

2019 ◽  
Vol 53 (6) ◽  
pp. 1900303 ◽  
Author(s):  
Amelia Shoemark ◽  
Erin Cant ◽  
Luis Carreto ◽  
Alexandria Smith ◽  
Martina Oriano ◽  
...  
Keyword(s):  
Neutrophil Elastase ◽  
Point Of Care ◽  
Severity Index ◽  
Forced Expiratory Volume ◽  
Lateral Flow ◽  
Elastase Activity ◽  
Lateral Flow Device ◽  
Airway Infection ◽  
Independent Validation ◽  
Flow Device

IntroductionNeutrophil elastase activity in sputum can identify patients at high risk of airway infection and exacerbations in bronchiectasis. Application of this biomarker in clinical practice is limited, because no point-of-care test is available. We tested whether a novel semi-quantitative lateral flow device (neutrophil elastase airway test stick – NEATstik®) can stratify bronchiectasis patients according to severity, airway infection and exacerbation risk.MethodsSputum samples from 124 patients with stable bronchiectasis enrolled in the UK and Spain were tested using the NEATstik®, which scores neutrophil elastase concentration from 0 (<8 µg·mL−1 elastase activity) to 10 (maximum detectable neutrophil elastase activity). High neutrophil elastase activity was regarded as a NEATstik® grade >6. Severity of disease, airway infection from sputum culture and exacerbations over the 12 months were recorded. An independent validation was conducted in 50 patients from Milan, Italy.Measurements and main resultsPatients had a median age of 69 years and forced expiratory volume in 1 s (FEV1) 69%. High neutrophil elastase activity was associated with worse bronchiectasis severity using the bronchiectasis severity index (p=0.0007) and FEV1 (p=0.02). A high NEATstik® grade was associated with a significant increase in exacerbation frequency, incident rate ratio 2.75 (95% CI 1.63–4.64, p<0.001). The median time to next exacerbation for patients with a NEATstik® grade >6 was 103 days compared to 278 days. The hazard ratio was 2.59 (95% CI 1.71–3.94, p<0.001). Results were confirmed in the independent validation cohort.ConclusionsA novel lateral flow device provides assessment of neutrophil elastase activity from sputum in minutes and identifies patients at increasing risk of airway infection and future exacerbations.

scholarly journals 1462Bronchoalveolar Lavage Lateral-Flow Device Test for Diagnosing Invasive Pulmonary Aspergillosis in ICU patients: a multicenter study

2014 ◽  
Vol 1 (suppl_1) ◽  
pp. S385-S386 ◽  
Author(s):  
Martin Hoenigl ◽  
Jürgen Prattes ◽  
Susanne Eigl ◽  
Cornelia Lass-Flörl ◽  
Birgit Willinger ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Invasive Pulmonary Aspergillosis ◽  
Lateral Flow ◽  
Pulmonary Aspergillosis ◽  
Icu Patients ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Device Test
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