A WRKY transcription factor, TaWRKY40‐D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat

Plant Biology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 1072-1085
Author(s):  
L. Zhao ◽  
W. Zhang ◽  
Q. Song ◽  
Y. Xuan ◽  
K. Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Jasmonic Acid ◽  
Leaf Senescence ◽  
Wrky Transcription Factor

scholarly journals The AP2/ERF Transcription Factor SlERF.F5 Functions in Leaf Senescence in Tomato

2021 ◽  
Author(s):  
Yanan Chen ◽  
Panpan Feng ◽  
Boyan Tang ◽  
Zongli Hu ◽  
Qiaoli Xie ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Jasmonic Acid ◽  
Leaf Senescence ◽  
Molecular Mechanisms ◽  
Receptor Gene ◽  
Signal Transduction Pathway ◽  
Ethylene Biosynthesis ◽  
Pcr Analysis ◽  
Ethylene Response Factor ◽  
Wild Type

Abstract The process of plant senescence is complex and highly coordinated, and is regulated by many endogenous and environmental signals. Ethylene and jasmonic acid are well-known senescence inducers, but their molecular mechanisms for inducing leaf senescence have not been fully elucidated. Here, we studied a receptor gene downstream of an ethylene signal transduction pathway, ETHYLENE RESPONSE FACTOR F5 (SlERF.F5). The silence of SlERF.F5 causes accelerated senescence induced by age, darkness, ethylene, and jasmonic acid. However, overexpression of SlERF.F5 may delay leaf senescence. We further found that silencing of SlERF.F5 inhibited the expression of chlorophyll-related genes CHLH, CHLM, POR, CAO1, GUN4, PPH, SGR1, RBCS, and AUREA genes, and the light-responsive RBCS and LHCA1 gene. Moreover, silencing of SlERF.F5 increases the sensitivity of SlERF.F5-RNAi lines to ethylene and jasmonic acid compared to wild type. In the dark-induced aging experiment, the qRT-PCR analysis showed the expression levels of genes related to the ethylene biosynthesis pathway and the jasmonic acid signaling pathway in SlERF.F5-RNAi lines increased compared with wild type. Yeast two-hybrid experiments showed that SlERF.F5 and SlMYC2 (a transcription factor downstream of the JA receptor) can interact physically, thereby mediating the role of SlERF.F5 in jasmonic acid-induced leaf senescence. Collectively, our research provides new insights into how ethylene and jasmonic acid promote leaf senescence in tomatoes.

scholarly journals Rice transcription factor OsMYB102 delays leaf senescence by down-regulating abscisic acid accumulation and signaling

2019 ◽  
Vol 70 (10) ◽  
pp. 2699-2715 ◽  
Author(s):  
Weilan Piao ◽  
Suk-Hwan Kim ◽  
Byoung-Doo Lee ◽  
Gynheung An ◽  
Yasuhito Sakuraba ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Leaf Senescence ◽  
Acid Accumulation

scholarly journals TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

2021 ◽  
Vol 12 ◽  
Author(s):  
Hualiang Qiao ◽  
Yongwei Liu ◽  
Lingling Cheng ◽  
Xuelin Gu ◽  
Pengcheng Yin ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Leaf Senescence ◽  
Brachypodium Distachyon ◽  
Wrky Transcription Factor ◽  
Luciferase Reporter ◽  
Virus Induced Gene Silencing ◽  
Reporter System ◽  
Mobility Shift ◽  
Yield And Quality ◽  
Mobility Shift Assay

Leaf senescence is crucial for crop yield and quality. Transcriptional regulation is a key step for integrating various senescence-related signals into the nucleus. However, few regulators of senescence implicating transcriptional events have been functionally characterized in wheat. Based on our RNA-seq data, we identified a WRKY transcription factor, TaWRKY13-A, that predominately expresses at senescent stages. By using the virus-induced gene silencing (VIGS) method, we manifested impaired transcription of TaWRKY13-A leading to a delayed leaf senescence phenotype in wheat. Moreover, the overexpression (OE) of TaWRKY13-A accelerated the onset of leaf senescence under both natural growth condition and darkness in Brachypodium distachyon and Arabidopsis thaliana. Furthermore, by physiological and molecular investigations, we verified that TaWRKY13-A participates in the regulation of leaf senescence via jasmonic acid (JA) pathway. The expression of JA biosynthetic genes, including AtLOX6, was altered in TaWRKY13-A-overexpressing Arabidopsis. We also demonstrated that TaWRKY13-A can interact with the promoter of AtLOX6 and TaLOX6 by using the electrophoretic mobility shift assay (EMSA) and luciferase reporter system. Consistently, we detected a higher JA level in TaWRKY13-A-overexpressing lines than that in Col-0. Moreover, our data suggested that TaWRKY13-A is partially functional conserved with AtWRKY53 in age-dependent leaf senescence. Collectively, this study manifests TaWRKY13-A as a positive regulator of JA-related leaf senescence, which could be a new clue for molecular breeding in wheat.

scholarly journals The Arabidopsis NAC Transcription Factor VNI2 Integrates Abscisic Acid Signals into Leaf Senescence via the COR/RD Genes

2011 ◽  
Vol 23 (6) ◽  
pp. 2155-2168 ◽  
Author(s):  
So-Dam Yang ◽  
Pil Joon Seo ◽  
Hye-Kyung Yoon ◽  
Chung-Mo Park
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Leaf Senescence ◽  
Nac Transcription Factor

scholarly journals ORA47 (octadecanoid‐responsive AP2/ERF‐domain transcription factor 47) regulates jasmonic acid and abscisic acid biosynthesis and signaling through binding to a novel cis ‐element

2016 ◽  
Vol 211 (2) ◽  
pp. 599-613 ◽  
Author(s):  
Hsing‐Yu Chen ◽  
En‐Jung Hsieh ◽  
Mei‐Chun Cheng ◽  
Chien‐Yu Chen ◽  
Shih‐Ying Hwang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Jasmonic Acid ◽  
Acid Biosynthesis ◽  
Abscisic Acid Biosynthesis ◽  
Cis Element
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