Stomatal function, density and pattern, and CO2 assimilation in Arabidopsis thaliana tmm1 and sdd1-1 mutants

Plant Biology ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 689-701 ◽  
Author(s):  
M. Vráblová ◽  
D. Vrábl ◽  
M. Hronková ◽  
J. Kubásek ◽  
J. Šantrůček
Keyword(s):  
Arabidopsis Thaliana ◽  
Co2 Assimilation ◽  
Function Density

scholarly journals Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

2020 ◽  
Author(s):  
Vasil Atanasov ◽  
Lisa Fürtauer ◽  
Thomas Nägele
Keyword(s):  
Arabidopsis Thaliana ◽  
Metabolic Regulation ◽  
Co2 Assimilation ◽  
High Light ◽  
Plant Performance ◽  
Hexokinase Activity ◽  
South Italy ◽  
Single Stress ◽  
Photosynthetic Capacities

Diurnal and seasonal changes of abiotic environmental factors shape plant performance and distribution. Changes of growth temperature and light intensity may vary significantly on a diurnal, but also on a weekly or seasonal scale. Hence, acclimation to a changing temperature and light regime is essential for plant survival and propagation. In the present study, we analyzed photosynthetic CO2 assimilation and metabolic regulation of the central carbohydrate metabolism in two natural accessions of Arabidopsis thaliana originating from Russia and south Italy during exposure to heat and a combination of heat and high light. Our findings indicate that it is hardly possible to predict photosynthetic capacities to fix CO2 under combined stress from single stress experiments. Further, capacities of hexose phosphorylation were found to be significantly lower in the Italian than in the Russian accession which could explain an inverted sucrose-to-hexose ratio. Together with the finding of significantly stronger accumulation of anthocyanins under heat/high light these observations indicate a central role of hexokinase activity in stabilization of photosynthetic capacities within a changing environment.

Stomatal aperture can compensate altered stomatal density in Arabidopsis thaliana at growth light conditions

2006 ◽  
Vol 33 (11) ◽  
pp. 1037 ◽  
Author(s):  
Dirk Büssis ◽  
Uritza von Groll ◽  
Joachim Fisahn ◽  
Thomas Altmann
Keyword(s):  
Arabidopsis Thaliana ◽  
Stomatal Conductance ◽  
Stomatal Density ◽  
Co2 Assimilation ◽  
High Light ◽  
Stomatal Aperture ◽  
Photochemical Quenching ◽  
Light Conditions ◽  
Wild Type ◽  
Light Intensities

Stomatal density of transgenic Arabidopsis thaliana plants over-expressing the SDD1 (stomatal density and distribution) gene was reduced to 40% and in the sdd1-1 mutant increased to 300% of the wild type. CO2 assimilation rate and stomatal conductance of over-expressers and the sdd1-1 mutant were unchanged compared with wild types when measured under the light conditions the plants were exposed to during growth. Lower stomatal density was compensated for by increased stomatal aperture and conversely, increased stomatal density was compensated for by reduced stomatal aperture. At high light intensities the assimilation rates and stomatal conductance of SDD1 over-expressers were reduced to 80% of those in wild type plants. Areas beneath stomata and patches lacking stomata were analysed separately. In areas without stomata, maximum fluorescence yield (Fv / Fm) and quantum yield of photosystem II (Φ PSII) were significantly lower than in areas beneath stomata. In areas beneath stomata, Fv / Fm and Φ PSII were identical to levels measured in wild type leaves. At high light intensities over-expressers showed decreased photochemical quenching (qP) compared with wild types. However, the decrease of qP was significantly stronger in areas without stomata than in mesophyll areas beneath stomata. At high CO2 partial pressures and high light intensities CO2 assimilation rates of SDD1 over-expressers did not reach wild type levels. These results indicate that photosynthesis in SDD1 over-expressers was reduced because of limiting CO2 in areas furthest from stomata at high light.

Distinct growth and physiological responses of Arabidopsis thaliana natural accessions to drought stress and their detection using spectral reflectance and thermal imaging

2017 ◽  
Vol 44 (3) ◽  
pp. 312 ◽  
Author(s):  
Karel Klem ◽  
Kumud B. Mishra ◽  
Kateřina Novotná ◽  
Barbora Rapantová ◽  
Petra Hodaňová ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Leaf Area ◽  
Thermal Imaging ◽  
Spectral Reflectance ◽  
Stomatal Closure ◽  
Substantial Reduction ◽  
Co2 Assimilation ◽  
Physiological Processes ◽  
Relative Water

Reduced growth and stomatal closure are the two main responses of plants to drought stress. The extent to which these processes are connected and whether different genotypes prefer one over the other remains unclear. To understand the genotype-specific interconnections of these two processes and evaluate potential utilisation of this knowledge for drought tolerance phenotyping, six natural accessions of Arabidopsis thaliana (L.) Heynh. were exposed to drought stress for 10 days. Projected leaf area of rosette, light-saturated CO2 assimilation rate (Amax), relative water content (RWC), leaf temperature (thermal imaging), and spectral reflectance were measured through the course of induced drought stress. Three types of acclimation were identified: (i) growth not affected but Amax significantly reduced, (ii) both growth and Amax significantly reduced, and (iii) growth significantly reduced but only small decrease in Amax. Within the last type, the smallest decline in RWC was evident. These results show that a substantial reduction in leaf area may cause a decline in transpiration that enables maintenance of both RWC and physiological processes. Both non-invasive thermal imaging and spectral reflectance measurements proved reliable tools for tracking drought-induced changes in Amax and RWC across all accessions tested and thus are effective tools for phenotyping stress tolerance.

scholarly journals Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 819
Author(s):  
Vasil Atanasov ◽  
Lisa Fürtauer ◽  
Thomas Nägele
Keyword(s):  
Arabidopsis Thaliana ◽  
Carbohydrate Metabolism ◽  
Metabolic Regulation ◽  
Environmental Changes ◽  
Co2 Assimilation ◽  
High Light ◽  
Plant Performance ◽  
Hexokinase Activity ◽  
South Italy

Diurnal and seasonal changes of abiotic environmental factors shape plant performance and distribution. Changes of growth temperature and light intensity may vary significantly on a diurnal, but also on a weekly or seasonal scale. Hence, acclimation to a changing temperature and light regime is essential for plant survival and propagation. In the present study, we analyzed photosynthetic CO2 assimilation and metabolic regulation of the central carbohydrate metabolism in two natural accessions of Arabidopsis thaliana that originate from north western Russia and south Italy during exposure to heat and a combination of heat and high light. Our findings indicate that it is hardly possible to predict photosynthetic capacities under combined stress from single stress experiments. Further, capacities of hexose phosphorylation were found to be significantly lower in the Italian than in the Russian accession, which could explain an inverted sucrose-to-hexose ratio. Together with the finding of significantly stronger accumulation of anthocyanins under heat/high light, these observations indicate a central role of hexokinase activity in the stabilization of photosynthesis and carbohydrate metabolism during environmental changes.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Late Flowering ◽  
Transition To Flowering

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene
Sign in / Sign up

Export Citation Format

Share Document