Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide

Plant Biology ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 973-979 ◽  
Author(s):  
M. Havé ◽  
L. Leitao ◽  
M. Bagard ◽  
J.-F. Castell ◽  
A. Repellin
Keyword(s):  
Winter Wheat ◽  
Leaf Senescence ◽  
Protein Carbonylation ◽  
Natural Leaf

scholarly journals Dismantling of Arabidopsis thaliana mesophyll cell chloroplasts during natural leaf senescence

Plant Biology ◽  
2010 ◽  
Vol 12 (1) ◽  
pp. 1-12 ◽  
Author(s):  
I. M. Evans ◽  
A. M. Rus ◽  
E. M. Belanger ◽  
M. Kimoto ◽  
J. A. Brusslan
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Senescence ◽  
Mesophyll Cell ◽  
Natural Leaf

scholarly journals SAUR49 Can Positively Regulate Leaf Senescence by Suppressing SSPP in Arabidopsis

2019 ◽  
Vol 61 (3) ◽  
pp. 644-658 ◽  
Author(s):  
Zewen Wen ◽  
Yuanyuan Mei ◽  
Jie Zhou ◽  
Yanjiao Cui ◽  
Dan Wang ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Protein Phosphatase ◽  
Functional Characterization ◽  
Transcript Level ◽  
In Planta ◽  
Environmental Signals ◽  
Receptor Like Kinase ◽  
Premature Leaf Senescence ◽  
Natural Leaf ◽  
Two Hybrid

Abstract The involvement of SMALL AUXIN-UP RNA (SAUR) proteins in leaf senescence has been more and more acknowledged, but the detailed mechanisms remain unclear. In the present study, we performed yeast two-hybrid assays and identified SAUR49 as an interactor of SENESCENCE SUPPRESSED PROTEIN PHOSPHATASE (SSPP), which is a PP2C protein phosphatase that negatively regulates Arabidopsis leaf senescence by suppressing the leucine-rich repeat receptor-like protein kinase SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK), as reported previously by our group. The interaction between SAUR49 and SSPP was further confirmed in planta. Functional characterization revealed that SAUR49 is a positive regulator of leaf senescence. The accumulation level of SAUR49 protein increased during natural leaf senescence in Arabidopsis. The transcript level of SAUR49 was upregulated during SARK-induced premature leaf senescence but downregulated during SSPP-mediated delayed leaf senescence. Overexpression of SAUR49 significantly accelerated both natural and dark-induced leaf senescence in Arabidopsis. More importantly, SAUR49 overexpression completely reversed SSPP-induced delayed leaf senescence. In addition, overexpression of SAUR49 reversed the decreased plasma membrane H+-ATPase activity mediated by SSPP. Taken together, the results showed that SAUR49 functions in accelerating the leaf senescence process via the activation of SARK-mediated leaf senescence signaling by suppressing SSPP. We further identified four other SSPP-interacting SAURs, SAUR30, SAUR39, SAUR41 and SAUR72, that may act redundantly with SAUR49 in regulating leaf senescence. All these observations indicated that certain members of the SAUR family may serve as an important hub that integrates various hormonal and environmental signals with senescence signals in Arabidopsis.

scholarly journals Leaf Senescence: The Chloroplast Connection Comes of Age

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 495 ◽  
Author(s):  
Martín L. Mayta ◽  
Mohammad-Reza Hajirezaei ◽  
Néstor Carrillo ◽  
Anabella F. Lodeyro
Keyword(s):  
Cell Death ◽  
Leaf Senescence ◽  
Developmental Process ◽  
Reproductive Organs ◽  
Common Thread ◽  
Dependent Cell ◽  
Redox Poise ◽  
Natural Leaf ◽  
As Stress ◽  
Stress Dependent

Leaf senescence is a developmental process critical for plant fitness, which involves genetically controlled cell death and ordered disassembly of macromolecules for reallocating nutrients to juvenile and reproductive organs. While natural leaf senescence is primarily associated with aging, it can also be induced by environmental and nutritional inputs including biotic and abiotic stresses, darkness, phytohormones and oxidants. Reactive oxygen species (ROS) are a common thread in stress-dependent cell death and also increase during leaf senescence. Involvement of chloroplast redox chemistry (including ROS propagation) in modulating cell death is well supported, with photosynthesis playing a crucial role in providing redox-based signals to this process. While chloroplast contribution to senescence received less attention, recent findings indicate that changes in the redox poise of these organelles strongly affect senescence timing and progress. In this review, the involvement of chloroplasts in leaf senescence execution is critically assessed in relation to available evidence and the role played by environmental and developmental cues such as stress and phytohormones. The collected results indicate that chloroplasts could cooperate with other redox sources (e.g., mitochondria) and signaling molecules to initiate the committed steps of leaf senescence for a best use of the recycled nutrients in plant reproduction.

scholarly journals Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower

2015 ◽  
Vol 14 (2) ◽  
pp. 719-734 ◽  
Author(s):  
Sebastián Moschen ◽  
Sofía Bengoa Luoni ◽  
Julio A. Di Rienzo ◽  
María del Pilar Caro ◽  
Takayuki Tohge ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Metabolomic Analysis ◽  
Natural Leaf

scholarly journals Identification of WRKY transcription factors associated with leaf and corolla senescence in Petunia hybrida

2021 ◽  
Author(s):  
Francisco Horacio Astigueta ◽  
Amilcar Hernán Baigorria ◽  
Martín Nahuel García ◽  
Verónica Cecilia Delfosse ◽  
Sergio Alberto González ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Leaf Senescence ◽  
Petunia Hybrida ◽  
Expression Profiles ◽  
Regulatory Element ◽  
Expression Data ◽  
Age Related ◽  
Weak Expression ◽  
Natural Leaf ◽  
Simultaneous Visualization

Abstract Several families of transcription factors (TFs) control the progression of senescence. Many key TFs belonging to the WRKY family have been described to play crucial roles in the regulation of leaf senescence, mainly in Arabidopsis. However, little is known about senescence-associated WRKY members in floricultural species. Delay of senescence in leaves and petals of Petunia hybrida, a worldwide ornamental crop are highly appreciated traits. In this work, starting from 28 differentially expressed WRKY genes of Arabidopsis during the progression of leaf senescence, we identified the orthologous in P. hybrida and explored the expression profiles of 20 PhWRKY genes during the progression of natural (age-related) leaf and corolla senescence as well as in the corollas of flowers undergoing pollination-induced senescence. Simultaneous visualization showed consistent and similar expression profiles of PhWRKYs during natural leaf and corolla senescence, although weak expression changes were observed during pollination-induced senescence. Comparable expression trends between PhWRKYs and the corresponding genes of Arabidopsis were observed during leaf senescence, although more divergences were found in petals of pollinated petunia flowers. Integration of expression data with phylogenetics, conserved motif and cis-regulatory element analyses were used to establish a list of solid candidates that could regulate more than one senescence process. Our results suggest that several members of the WRKY family of TFs are tightly linked to the regulation of senescence in P. hybrida.

Disentangling the photosynthesis performance in japonica rice during natural leaf senescence using OJIP fluorescence transient analysis

2021 ◽  
Vol 48 (2) ◽  
pp. 206
Author(s):  
Faliang Zeng ◽  
Guojiao Wang ◽  
Yinpei Liang ◽  
Naihui Guo ◽  
Lin Zhu ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Grain Filling ◽  
Japonica Rice ◽  
Yield And Quality ◽  
Test Parameters ◽  
Electron Transport Chains ◽  
Rice Genotypes ◽  
Ojip Fluorescence Transient ◽  
Natural Leaf

Rice undergoes leaf senescence accompanied with grain filling when the plants reach the end of their temporal niche, and a delay in leaf senescence ultimately improves the yield and quality of grain. To estimate the decline in photosynthesis during leaf senescence and to find an efficient and useful tool to identify rice genotypes with a longer duration of active photosynthesis, we examined PSII photosynthetic activity in the flag leaves of japonica rice Shennong265 (SN265) and Beigeng3 (BG3) during leaf senescence using chlorophyll a fluorescence kinetics. The results show that inhibition occurred in the electron transport chains, but the energetic connectivity of PSII units was not affected as dramatically during leaf senescence. PSII reaction centres (RCs) were transformed into ‘silent RCs,’ and the chlorophyll content decreased during leaf senescence. However the size of the ‘economic’ antennae increased. Further, the percentage of variation of the specific energy flux parameters can rationally be used to indicate leaf senescence from the perspective of energy balance. Although the performance indices were more sensitive than other functional and structural JIP-test parameters, they still did not serve as an indicator of crop yield.

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