Profiles of embryonic nuclear protein binding to the proximal promoter region of the soybean β-conglycinin α subunit gene

Plant Biology ◽  
2014 ◽  
Vol 17 (1) ◽  
pp. 147-152 ◽  
Author(s):  
M. Yoshino ◽  
K. Tsutsumi ◽  
A. Kanazawa
Keyword(s):  
Protein Binding ◽  
Promoter Region ◽  
Nuclear Protein ◽  
Proximal Promoter ◽  
Subunit Gene ◽  
Α Subunit ◽  
Proximal Promoter Region

scholarly journals GC- and E-box Motifs as Regulatory Elements in the Proximal Promoter Region of the Neuronal Nicotinic Receptor α7 Subunit Gene

1998 ◽  
Vol 273 (32) ◽  
pp. 20021-20028 ◽  
Author(s):  
Carmen Carrasco-Serrano ◽  
Antonio Campos-Caro ◽  
Salvador Viniegra ◽  
Juan J. Ballesta ◽  
Manuel Criado
Keyword(s):  
Nicotinic Receptor ◽  
Promoter Region ◽  
Regulatory Elements ◽  
Proximal Promoter ◽  
Subunit Gene ◽  
Proximal Promoter Region ◽  
Neuronal Nicotinic Receptor ◽  
E Box ◽  
Α7 Subunit

scholarly journals Analysis of proteins binding to the proximal promoter region of two rat serine protease inhibitor genes

1992 ◽  
Vol 20 (5) ◽  
pp. 1061-1068 ◽  
Author(s):  
Valerie Rossi ◽  
Jean Francois Rouayrenc ◽  
Laurent Paquereau ◽  
Marie Joseé Vilarem ◽  
Alphonse Le Cam
Keyword(s):  
Protease Inhibitor ◽  
Serine Protease ◽  
Promoter Region ◽  
Serine Protease Inhibitor ◽  
Proximal Promoter ◽  
Proximal Promoter Region ◽  
Inhibitor Genes

AVP-induced VIT32 gene expression in collecting duct cells occurs via trans-activation of a CRE in the 5′-flanking region of the VIT32 gene

2004 ◽  
Vol 287 (3) ◽  
pp. F460-F468 ◽  
Author(s):  
Christie P. Thomas ◽  
Randy W. Loftus ◽  
Kang Z. Liu
Keyword(s):  
Gene Expression ◽  
Promoter Region ◽  
Collecting Duct ◽  
Airway Epithelial Cells ◽  
Proximal Promoter ◽  
Xenopus Laevis Oocytes ◽  
Proximal Promoter Region ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Flanking Region

VIT32, a vasopressin-induced transcript, inhibits Na+ transport when coexpressed with the epithelial sodium channel in Xenopus laevis oocytes ( EMBO J 21: 5109–5117, 2002). To understand the mechanism of VIT32 gene regulation, we examined the effect of DDAVP and cAMP stimulation on VIT32 expression in M-1 mouse collecting duct cells and in H441 human airway epithelial cells. Elevation of cAMP with forskolin and IBMX increased VIT32 gene expression with a peak effect at 2 h. The increase in gene expression was abolished by H89 and by actinomycin D, suggesting that cAMP stimulates VIT32 mRNA expression by a PKA-mediated increase in gene transcription. An ∼1.5-kb fragment of the 5′-flanking region of VIT32 was cloned and was able to confer cAMP-stimulated reporter gene activity when transfected into M-1 and H441 cells. By deletion analysis and site-directed mutagenesis, a cAMP response element (CRE) was identified within the proximal promoter region that was sufficient to account for the increase in VIT32 gene expression seen with DDAVP and elevation of cAMP. Furthermore, DDAVP-stimulated VIT32 promoter-reporter activity was inhibited by H89 and by a dominant negative CREB construct. Finally, we were able to identify CREB as a nuclear protein that bound to the VIT32 CRE in gel mobility shift assays. In summary, DDAVP stimulates transcription of VIT32 via a CRE within the proximal promoter region of the VIT32 gene.

Functional interactions of an upstream enhancer of the mouse glycoprotein hormone α-subunit gene with proximal promoter sequences

1998 ◽  
Vol 142 (1-2) ◽  
pp. 141-152 ◽  
Author(s):  
William M Wood ◽  
Janet M Dowding ◽  
Virginia D Sarapura ◽  
Michael T McDermott ◽  
David F Gordon ◽  
...  
Keyword(s):  
Proximal Promoter ◽  
Subunit Gene ◽  
Glycoprotein Hormone ◽  
Α Subunit ◽  
Promoter Sequences ◽  
Functional Interactions
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