Gastritis cystica profunda is associated with aberrant p53 and Epstein–Barr virus in gastric cancer: A clinicopathological, immunohistochemical and in situ hybridization study

2020 ◽  
Author(s):  
Hiroe Itami ◽  
Kohei Morita ◽  
Tokiko Nakai ◽  
Tomoko Uchiyama ◽  
Sumire Sugimoto ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Hybridization Study ◽  
Barr Virus ◽  
Epstein Barr ◽  
Gastritis Cystica Profunda

Epstein-Barr virus associated gastric carcinoma at the United States-Mexico border: A single institution study.

2020 ◽  
Vol 38 (4_suppl) ◽  
pp. 440-440
Author(s):  
Sumit Gaur ◽  
Ramadevi Subramani ◽  
Meghan Mcalice ◽  
Osvaldo Padilla ◽  
Brenda Sofia Castillo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
El Paso ◽  
Safety Net ◽  
Routine Testing ◽  
Barr Virus ◽  
Epstein Barr ◽  
H Pylori

440 Background: Globally, gastric cancer (GC) is the fourth most prevalent cancer, and the second leading cause of cancer related deaths. Epstein-Barr virus is implicated in the pathogenesis of 5-10% of gastric cancers. Based upon the results obtained from of the cancer genome atlas, EBV related GC is characterized by promoter hypermethylation, PIK3CA mutations (80%) and increased expression of PD-1 and PD-L1, making it an attractive target for molecularly targeted therapy and immunotherapeutic options. As such, a case can be made for routine testing for EBV in all GC patients. University medical center, El Paso is a tax payer funded safety net health system in El Paso country, TX. We conducted a pilot study to characterize the prevalence of EBV associated gastric cancer seen at this facility. Methods: After obtaining institutional review board (IRB) approval, we identified cases of GC that were diagnosed between January 1, 2008- and December 31-2017. A total of 104 cases were identified of which 17 samples were randomly selected. Pathology specimens were reviewed to identify grade, subtype (intestinal vs diffuse), degree of lymphocytic infiltration and presence/absence of H. pylori. Representative sections from archived tumors were used to perform in-situ hybridization to look for the presence of Epstein-Barr virus. Samples were analyzed using the Rembrandt In situ Hybridization and Detection Universal RISH& HRP Detection Kit for Epstein-Barr early RNA. Results: The median age of the 17 patients is 63 years with 59% being males. 95% self identified as Hispanic. 41% were smokers, 18% used alcohol. The mean BMI was 27.3. Forty one percent of gastric cancer cases were found in the body, 29% in the antrum, 12% in the cardia, and 6% in the fundus. Forty one percent of cases were Stage IV, 24% stage II, 17% Stage III and 17% Stage I. 95% of cases were high grade, 53% of them had signet ring features. 18% of samples were H. pylori positive. None of the seventeen samples tested positive for EBV. Conclusions: EBV does not seem to contribute significantly to the pathogenesis of gastric cancer in our local population. As such routine testing for EBV in all gastric cancer patients may not be a cost effective utilization of resources at our hospital.

scholarly journals Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection

2012 ◽  
Vol 43 (1) ◽  
pp. 393-404 ◽  
Author(s):  
Marcos Antonio Pereira de Lima ◽  
Márcia Valéria Pitombeira Ferreira ◽  
Marcos Aurélio Pessoa Barros ◽  
Maria Inês de Moura Campos Pardini ◽  
Adriana Camargo Ferrasi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma

Blood ◽  
2007 ◽  
Vol 110 (3) ◽  
pp. 972-978 ◽  
Author(s):  
Sarah Park ◽  
Jeeyun Lee ◽  
Young Hyeh Ko ◽  
Arum Han ◽  
Hyun Jung Jun ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
B Cell ◽  
Epstein Barr Virus ◽  
Progression Free Survival ◽  
B Cell Lymphoma ◽  
Free Survival ◽  
Barr Virus ◽  
Large B Cell Lymphoma ◽  
Epstein Barr

AbstractTo define prognostic impact of Epstein-Barr virus (EBV) infection in diffuse large B-cell lymphoma (DLBCL), we investigated EBV status in patients with DLBCL. In all, 380 slides from paraffin-embedded tissue were available for analysis by EBV-encoded RNA-1 (EBER) in situ hybridization, and 34 cases (9.0%) were identified as EBER-positive. EBER positivity was significantly associated with age greater than 60 years (P = .005), more advanced stage (P < .001), more than one extranodal involvement (P = .009), higher International Prognostic Index (IPI) risk group (P = .015), presence of B symptom (P = .004), and poorer outcome to initial treatment (P = .006). The EBER+ patients with DLBCL demonstrated substantially poorer overall survival (EBER+ vs EBER− 35.8 months [95% confidence interval (CI), 0-114.1 months] vs not reached, P = .026) and progression-free survival (EBER+ vs EBER− 12.8 months [95% CI, 0-31.8 months] vs 35.8 months [95% CI, 0-114.1 months], respectively (P = .018). In nongerminal center B-cell–like subtype, EBER in situ hybridization positivity retained its statistical significance at the multivariate level (P = .045). Nongerminal center B-cell–like patients with DLBCL with EBER positivity showed substantially poorer overall survival with 2.9-fold (95% CI, 1.1-8.1) risk for death. Taken together, DLBCL patients with EBER in situ hybridization+ pursued more rapidly deteriorating clinical course with poorer treatment response, survival, and progression-free survival.

Detection of Epstein–Barr virus in children's adenoids by in situ hybridization

2003 ◽  
Vol 1257 ◽  
pp. 157-160 ◽  
Author(s):  
L.H. Endo ◽  
E. Sakano ◽  
L.A. Camargo ◽  
D.R. Ferreira ◽  
G.A. Pinto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Nasal Natural Killer Cell/T-Cell Lymphoma Showing Cellular Morphology Mimicking Normal Lymphocytes

2002 ◽  
Vol 126 (5) ◽  
pp. 602-605
Author(s):  
Katsuya Chinen ◽  
Yasuhiko Kaneko ◽  
Toshiyuki Izumo ◽  
Yasuo Ohkura ◽  
Osamu Matsubara ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
Natural Killer ◽  
Epstein Barr Virus ◽  
Cell Lymphoma ◽  
Normal Lymphocyte ◽  
Lymphoma Cells ◽  
Barr Virus ◽  
Epstein Barr

Abstract We report the autopsy case of a 34-year-old Japanese man with a nasal natural killer (NK)-cell/T-cell lymphoma. The patient developed the disease at 32 years of age, and a biopsy of the nasopharynx revealed pleomorphic lymphoma cell proliferation. Radiotherapy was performed, but the patient eventually died of respiratory failure. After radiotherapy, no histologic evidence of malignancy was obtained with biopsy materials featuring lymphocytic infiltration. Autopsy studies, including in situ hybridization for Epstein-Barr virus–encoded RNA, revealed generalized infiltration of normal lymphocyte-like, UCHL-1–positive, and Epstein-Barr virus–encoded RNA–positive lymphoma cells. Monoclonal proliferation of the Epstein-Barr virus–carrying cells was verified by means of Southern blot analysis. Retrospectively, we concluded that the normal lymphocyte-like presentation of the lymphoma cells, probably influenced by radiotherapy, prevented pathologists from recognizing the lymphoma. The utility of in situ hybridization for Epstein-Barr virus–encoded RNA in identification of tumor cells is emphasized with respect to the present case.

scholarly journals In situ hybridization for the detection of Epstein–Barr virus in central nervous system lymphomas

Cancer ◽  
1991 ◽  
Vol 67 (2) ◽  
pp. 444-448 ◽  
Author(s):  
Raouf E. Nakhleh ◽  
J. Carlos Manivel ◽  
Cedith M. Copenhaver ◽  
Joo H. Sung ◽  
John G. Strickler
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr
Sign in / Sign up

Export Citation Format

Share Document