Immunoproteomic analysis of fish ectoparasite, Argulus siamensis antigens

2021 ◽  
Author(s):  
Priyanka Das ◽  
Mohan R. Badhe ◽  
Pramoda Kumar Sahoo ◽  
Raudu Rajendra Kumar Reddy ◽  
Amol R. Suryawanshi ◽  
...  
Keyword(s):  
Argulus Siamensis

Efficacy of two avermectins, doramectin and ivermectin against Argulus siamensis infestation in Indian major carp, Labeo rohita

2012 ◽  
Vol 190 (1-2) ◽  
pp. 297-304 ◽  
Author(s):  
K.P. Hemaprasanth ◽  
Banya Kar ◽  
S.K. Garnayak ◽  
J. Mohanty ◽  
J.K. Jena ◽  
...  
Keyword(s):  
Labeo Rohita ◽  
Indian Major Carp ◽  
Argulus Siamensis

Feeding Apparatus and Associated Glands in the Freshwater Fish Ectoparasite Argulus Siamensis Wilson, 1926 (Branchiura)

Crustaceana ◽  
2011 ◽  
Vol 84 (10) ◽  
pp. 1153-1168 ◽  
Keyword(s):  
Buccal Cavity ◽  
Functional Relation ◽  
Giant Cells ◽  
Tropical Fish ◽  
Secretory Vesicles ◽  
Feeding Apparatus ◽  
Structural Details ◽  
Vasodilatory Activity ◽  
Argulus Siamensis ◽  
Oral Spine

AbstractThis study reports on the anatomy and structural details of the feeding apparatus and associated glands in a tropical fish ectoparasite, Argulus siamensis Wilson, 1926. The results reveal that anatomically the feeding apparatus of the species consists of a proximal buccal proboscis and a retractile pre-oral spine surrounded by a sheath. The mouth is located at the terminal end of the buccal proboscis. Two types of glands, the spinal gland and the proboscis gland, are associated with the feeding apparatus. The sac-like spinal gland, consisting of four polygonal cells, is located at the base of the spine. It is continuous with the median duct of the spine but does not show any connection with the buccal cavity. Numerous secretory vesicles are present in the cytoplasm. The proboscis glands are located parallel to the spinal sheath, one on both sides, and open directly into the buccal proboscis. Each proboscis gland houses two giant cells, the cytoplasm of which is largely occupied by secretory vesicles. The present study, aimed at understanding the functional relation of the glandular secretions with the feeding mechanism, revealed that the secretion of the spinal gland is neither cytotoxic nor haemorrhagic, but may have anaesthetic or vasodilatory activity. Indications are available to show that the secretion of the proboscis gland is anticoagulant in nature.

Development of a western blot method for detection of fish ectoparasite Argulus siamensis antigens

2018 ◽  
Vol 39 (4) ◽  
pp. 439-450
Author(s):  
P. Das ◽  
J. Mohanty ◽  
M.R. Badhe ◽  
P.K. Sahoo ◽  
K.K. Sardar ◽  
...  
Keyword(s):  
Western Blot ◽  
Western Blot Method ◽  
Argulus Siamensis

scholarly journals Evaluation of ribosomal P0 peptide as a vaccine candidate against Argulus siamensis in Labeo rohita

2017 ◽  
Vol 12 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Banya Kar ◽  
Amruta Mohapatra ◽  
Jyotirmaya Mohanty ◽  
Pramoda Kumar Sahoo
Keyword(s):  
Post Infection ◽  
Vaccine Candidate ◽  
Labeo Rohita ◽  
Vaccine Antigen ◽  
Control Group ◽  
Sequence Information ◽  
Promising Alternative ◽  
Significant Difference ◽  
P0 Protein ◽  
Argulus Siamensis

AbstractArgulusspp. are important ectoparasites of fish, and the current approach of their control using chemical pesticides has numerous drawbacks. Vaccination is a promising alternative but identification of protective antigens is a limiting step. The ribosomal protein P0, essential for protein synthesis, has been studied as a vaccine candidate. We generated sequence information of the P0 protein of the ectoparasiteArgulus siamensisand the hostLabeo rohita. The region of the parasite P0 protein with less sequence similarity with that of the host P0 protein and high predicted antigenicity was used for peptide synthesis. The peptide was conjugated with keyhole limpet hemocyanin (KLH) for immunization of rohu at a dose of 1.5 μg/g body weight. Dot blot assays confirmed production of antibodies against pP0-KLH in immunized fish. We evaluated the efficiency of pP0-KLH as a vaccine antigen by challenge of the immunized fish withA.siamensis. Although there was no significant difference in parasite load between both groups, a reduced and delayed mortality of 59% (15 days post-infection) in immunized group was noticed as compared to 75% mortality (within 7–15 days post-infection) in control group. The partial protection observed indicated the need for further optimization of this molecule to develop it into a vaccine candidate.

Mixed infection of Argulus japonicus and Argulus siamensis (Branchiura, Argulidae) in carps (Pisces, Cyprinidae): loss estimation and a comparative invasive pattern study

Crustaceana ◽  
2012 ◽  
Vol 85 (12-13) ◽  
pp. 1449-1462 ◽  
Author(s):  
◽  
Jyotirmaya Mohanty ◽  
Pramoda K. Sahoo ◽  
Sushil K. Garnayak ◽  
Banya Kar
Keyword(s):  
Mixed Infection ◽  
Loss Estimation ◽  
Argulus Siamensis

scholarly journals Egg laying strategies and effect of temperature on egg development of Argulus siamensis

2012 ◽  
Vol 37 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Pramoda Kumar Sahoo ◽  
Jyotirmaya Mohanty ◽  
Hemaprasanth ◽  
Banya Kar ◽  
Bikash Ranjan Mohanty ◽  
...  
Keyword(s):  
Egg Laying ◽  
Effect Of Temperature ◽  
Egg Development ◽  
Argulus Siamensis

Variation in susceptibility pattern of fish to Argulus siamensis: Do immune responses of host play a role?

2016 ◽  
Vol 221 ◽  
pp. 76-83 ◽  
Author(s):  
Banya Kar ◽  
Cisse Moussa ◽  
Amruta Mohapatra ◽  
Jyotirmaya Mohanty ◽  
Pallipuram Jayasankar ◽  
...  
Keyword(s):  
Immune Responses ◽  
Susceptibility Pattern ◽  
Argulus Siamensis

First evidence of comparative responses of Toll-like receptor 22 (TLR22) to relatively resistant and susceptible Indian farmed carps to Argulus siamensis infection

2014 ◽  
Vol 47 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Rudra Prasanna Panda ◽  
V. Chakrapani ◽  
Swagat Kumar Patra ◽  
Jatindra Nath Saha ◽  
Pallipuram Jayasankar ◽  
...  
Keyword(s):  
Toll Like Receptor ◽  
Argulus Siamensis
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