Trichomonas vaginalis ‐secreted cysteinyl leukotrienes promote migration, degranulation and MCP‐1 production in mast cells

2020 ◽  
Vol 42 (12) ◽  
Author(s):  
Young Ah Lee ◽  
Young Hee Nam ◽  
Arim Min ◽  
Myeong Heon Shin
Keyword(s):  
Mast Cells ◽  
Trichomonas Vaginalis ◽  
Cysteinyl Leukotrienes

scholarly journals SNAP23-Dependent Surface Translocation of Leukotriene B4 (LTB4) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB4

2016 ◽  
Vol 85 (1) ◽  
Author(s):  
Arim Min ◽  
Young Ah Lee ◽  
Kyeong Ah Kim ◽  
Jamel El-Benna ◽  
Myeong Heon Shin
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Trichomonas Vaginalis ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Ros Generation ◽  
Physical Interaction ◽  
Content Type ◽  
Receptor Complexes ◽  
Human Mast Cells

ABSTRACT Trichomonas vaginalis is a sexually transmitted parasite that causes vaginitis in women and itself secretes lipid mediator leukotriene B4 (LTB4). Mast cells are important effector cells of tissue inflammation during infection with parasites. Membrane-bridging SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes are critical for fusion during exocytosis. Although T. vaginalis-derived secretory products (TvSP) have been shown to induce exocytosis in mast cells, information regarding the signaling mechanisms between mast cell activation and TvSP is limited. In this study, we found that SNAP23-dependent surface trafficking of LTB4 receptor 1 (BLT1) is required for nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2)-mediated exocytotic degranulation of mast cells induced by TvSP. First, stimulation with TvSP induced exocytotic degranulation and reactive oxygen species (ROS) generation in HMC-1 cells. Next, TvSP-induced ROS generation and exocytosis were strongly inhibited by transfection of BLT1 small interfering RNA (siRNA). TvSP induced trafficking of BLT1 from the cytosol to the plasma membrane. We also found that knockdown of SNAP23 abrogated TvSP-induced ROS generation, exocytosis, and surface trafficking of BLT1 in HMC-1 cells. By coimmunoprecipitation, there was a physical interaction between BLT1 and SNAP23 in TvSP-stimulated HMC-1 cells. Taken together, our results suggest that SNAP23-dependent surface trafficking of BLT1 is essential for exocytosis in human mast cells induced by T. vaginalis-secreted LTB4. Our data collectively demonstrate a novel regulatory mechanism for SNAP23-dependent mast cell activation of T. vaginalis-secreted LTB4 involving surface trafficking of BLT1. These results can help to explain how the cross talk mechanism between parasite and host can govern deliberately tissue inflammatory responses.

scholarly journals Cysteinyl Leukotrienes Enhance the Degranulation of Bone Marrow-Derived Mast Cells through the Autocrine Mechanism

2009 ◽  
Vol 217 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Izumi Kaneko ◽  
Kaori Suzuki ◽  
Kaori Matsuo ◽  
Hiroyuki Kumagai ◽  
Yuji Owada ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Cysteinyl Leukotrienes ◽  
Autocrine Mechanism

scholarly journals Expression of the type 2 receptor for cysteinyl leukotrienes (CysLT2R) by human mast cells: Functional distinction from CysLT1R

2003 ◽  
Vol 100 (20) ◽  
pp. 11589-11593 ◽  
Author(s):  
E. A. Mellor ◽  
N. Frank ◽  
D. Soler ◽  
M. R. Hodge ◽  
J. M. Lora ◽  
...  
Keyword(s):  
Mast Cells ◽  
Cysteinyl Leukotrienes ◽  
Human Mast Cells
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