Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

2020 ◽  
Vol 42 (6) ◽  
Author(s):  
Mara Gutiérrez‐Sánchez ◽  
Maria Maricela Carrasco‐Yepez ◽  
Jorge Herrera‐Díaz ◽  
Saul Rojas‐Hernández
Keyword(s):  
Protein Recognition ◽  
Naegleria Fowleri ◽  
Differential Protein ◽  
Recognition Pattern ◽  
Nægleria Fowleri ◽  
Naegleria Lovaniensis

Cell surface differences of Naegleria fowleri and Naegleria lovaniensis exposed with surface markers

2007 ◽  
Vol 117 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Arturo González-Robles ◽  
Guadalupe Castañón ◽  
Ana Ruth Cristóbal-Ramos ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Maritza Omaña-Molina ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Markers ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
Naegleria Lovaniensis

Efficacy of a combined system of copper and silver and free chlorine for inactivation of Naegleria fowleri amoebas in water

1995 ◽  
Vol 31 (5-6) ◽  
pp. 119-122 ◽  
Author(s):  
J. M. Cassells ◽  
M. T. Yahya ◽  
C. P. Gerba ◽  
J. B. Rose
Keyword(s):  
Synergistic Effect ◽  
Exposure Time ◽  
Silver Ions ◽  
Free Chlorine ◽  
Log10 Reduction ◽  
Naegleria Fowleri ◽  
Combined System ◽  
Water Ph ◽  
Nægleria Fowleri ◽  
Time Required

Electrolytically generated copper and silver ions (400:40 and 800:80 μg/l) were evaluated, separately and combined with 1.0 mg/l free chlorine, for their efficacy in reducing the viable numbers of Naegleria fowleri amoebas in water (pH 7.3 and 23-25°C). Inactivation rates (k = log10 reduction/min) and T99 values (exposure time required to achieve a 99% or a 2 log10 reduction) of the disinfectants were determined. Copper and silver alone, at ratio of 400:40 to 800:80 μg/l caused no significant inactivation of N. fowleri even after 72 hours of exposure (k = 0.00017 and 0.00013, respectively). Addition of 1.0 mg/l free chlorine to water which contained 400:40 or 800:80 μg/l copper and silver resulted in enhanced inactivation rates (k = 0.458 and 0.515, respectively) compared to either chlorine alone (k = 0.33) or the metals alone. Water containing 800:80 μg/l copper and silver with 1.0 mg/l chlorine showed a T99 value of 3.9 minutes, while chlorine alone showed a T99 of 6.1 minutes. Enhanced inactivation of N. fowleri by a combined system of free chlorine and copper and silver may be attributed to the different mechanism that each disinfectant utilizes in inactivating the amoebas, and may suggest a synergistic effect.

scholarly journals A Novel Cysteine Protease Inhibitor of Naegleria fowleri That Is Specifically Expressed during Encystation and at Mature Cysts

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 388
Author(s):  
Hương Giang Lê ◽  
A-Jeong Ham ◽  
Jung-Mi Kang ◽  
Tuấn Cường Võ ◽  
Haung Naw ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Cysteine Protease ◽  
Expression Profiles ◽  
Critical Role ◽  
Cyst Formation ◽  
Cysteine Proteases ◽  
Cysteine Protease Inhibitor ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
Cystatin Family

Naegleria fowleri is a free-living amoeba that is ubiquitous in diverse natural environments. It causes a fatal brain infection in humans known as primary amoebic meningoencephalitis. Despite the medical importance of the parasitic disease, there is a great lack of knowledge about the biology and pathogenicity of N. fowleri. In this study, we identified and characterized a novel cysteine protease inhibitor of N. fowleri (NfCPI). NfCPI is a typical cysteine protease inhibitor belonging to the cystatin family with a Gln-Val-Val-Ala-Gly (QVVAG) motif, a characteristic motif conserved in the cystatin family of proteins. Bacterially expressed recombinant NfCPI has a dimeric structure and exhibits inhibitory activity against several cysteine proteases including cathespin Bs of N. fowleri at a broad range of pH values. Expression profiles of nfcpi revealed that the gene was highly expressed during encystation and cyst of the amoeba. Western blot and immunofluorescence assays also support its high level of expression in cysts. These findings collectively suggest that NfCPI may play a critical role in encystation or cyst formation of N. fowleri by regulating cysteine proteases that may mediate encystation or mature cyst formation of the amoeba. More comprehensive studies to investigate the roles of NfCPI in encystation and its target proteases are necessary to elucidate the regulatory mechanism and the biological significance of NfCPI.

Transmission of Naegleria fowleri between Mice

1983 ◽  
Vol 69 (1) ◽  
pp. 249 ◽  
Author(s):  
R. G. May ◽  
D. T. John
Keyword(s):  
Naegleria Fowleri ◽  
Nægleria Fowleri

Development of a rapid DNA extraction method and one-step nested PCR for the detection of Naegleria fowleri from the environment

2011 ◽  
Vol 45 (16) ◽  
pp. 5211-5217 ◽  
Author(s):  
Arine Fadzlun Ahmad ◽  
James Lonnen ◽  
Peter W. Andrew ◽  
Simon Kilvington
Keyword(s):  
Dna Extraction ◽  
Nested Pcr ◽  
Extraction Method ◽  
Naegleria Fowleri ◽  
Dna Extraction Method ◽  
One Step ◽  
Nægleria Fowleri

Development of a high- versus low-pathogenicity model of the free-living amoeba Naegleria fowleri

Microbiology ◽  
2012 ◽  
Vol 158 (10) ◽  
pp. 2652-2660 ◽  
Author(s):  
Denise C. Burri ◽  
Bruno Gottstein ◽  
Béatrice Zumkehr ◽  
Andrew Hemphill ◽  
Nadia Schürch ◽  
...  
Keyword(s):  
Naegleria Fowleri ◽  
Free Living ◽  
Free Living Amoeba ◽  
Nægleria Fowleri
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