scholarly journals Human eosinophils modulate peripheral blood mononuclear cell response to Schistosoma mansoni adult worm antigen in vitro

2016 ◽  
Vol 38 (8) ◽  
pp. 516-522 ◽  
Author(s):  
R. Tweyongyere ◽  
H. Namanya ◽  
P. Naniima ◽  
S. Cose ◽  
E. M. Tukahebwa ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cell ◽  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Cell Response ◽  
Peripheral Blood Mononuclear ◽  
Adult Worm Antigen

scholarly journals Efek Pemberian Protein Rekombinan Fusi ESAT6-CFP10 Mycobacterium tuberculosis terhadap Persentase IL2 dan IL10 yang Dipresentasikan Sel T CD8 pada Kultur PBMC

2019 ◽  
Vol 30 (3) ◽  
pp. 202
Author(s):  
David Christianto ◽  
Tri Yudani Mardining Raras ◽  
Sumarno Sumarno ◽  
Maimun Zulhaidah Arthamin ◽  
Triwahju Astuti ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear

<p><br />Keberhasilan vaksin BCG dalam memberikan perlindungan terhadap tuberkulosis (TB) pada orang dewasa di Indonesia belum optimal (37%) sehingga diperlukan vaksin alternatif yang lebih efektif. Protein rekombinan fusi ESAT6-CFP10 merupakan kandidat vaksin yang potensial. Penelitian dilakukan untuk menguji efektifitas protein rekombinan fusi ESAT6-CFP10 dalam meningkatkan ekspresi IL2 dan IL10 sel T CD8 yang memainkan peran penting dalam respon imun melawan TB. Pengujian kandidat vaksin dilakukan secara in vitro pada peripheral blood mononuclear cell (PBMC) dari kelompok sehat endemik TB, kelompok kontak TB, dan kelompok pasien TB dengan melihat persentase IL2 dan IL10 CD8. Setiap kelompok diberi perlakuan tanpa antigen, PPD, dan protein rekombinan fusi ESAT6-CFP10. Persentase IL2 meningkat secara signifikan dari kelompok sehat, kontak TB, hingga Pasien TB. Sebaliknya peningkatan persentase IL2 antar kelompok yang dipaparkan PPD tidak signifikan secara statistik (p=0,396). Persentase IL10 tidak menunjukkan perbedaan yang signifikan antar kelompoknya baik tanpa paparan antigen (p=0,617), PPD (p=0,351), maupun protein rekombinan fusi ESAT6-CFP10 (p=0,257). Didapatkan persentase IL2 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,309), kelompok kontak TB (p=0,318), dan kelompok pasien TB (p=0,424). Demikian juga dengan persentase IL10 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,908), kelompok kontak TB (p=0,352), dan kelompok pasien TB (p=0,776). Hal ini menunjukkan bahwa protein fusi rekombinan ESAT6-CFP10 dapat meningkatkan persentase IL2 tetapi tidak dengan IL10 meskipun secara statistik tidak signifikan.</p>

scholarly journals Plasma Interleukin-12 in Malaria-Tolerant Papua New Guineans: Inverse Correlation with Plasmodium falciparum Parasitemia and Peripheral Blood Mononuclear Cell Nitric Oxide Synthase Activity

2003 ◽  
Vol 71 (11) ◽  
pp. 6354-6357 ◽  
Author(s):  
Craig S. Boutlis ◽  
Moses Lagog ◽  
Sujittra Chaisavaneeyakorn ◽  
Mary A. Misukonis ◽  
Moses J. Bockarie ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Plasmodium Falciparum ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Interleukin 12 ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
Twofold Increase

ABSTRACT Interleukin-12 (IL-12) has been inversely associated with disease severity in human and murine malaria, and a polymorphism in the IL-12 p40 subunit gene (IL12B) has been associated with susceptibility to human cerebral malaria and reduced nitric oxide (NO) production. To better define the relationships between IL-12, NO, malaria parasitemia, and IL12B polymorphisms during malarial tolerance, plasma IL-12 levels and peripheral blood mononuclear cell NO synthase (NOS) activity were measured in asymptomatic Papua New Guineans exposed to intense malaria transmission. The IL-12 level was strongly inversely correlated with the density of Plasmodium falciparum parasitemia (ρ = −0.45; P < 0.001) and was predicted to decrease by 19% (95% confidence interval [CI], 10 to 27%) for each twofold increase in P. falciparum parasitemia. This is consistent with a suppressive effect of parasitemia on IL-12 production, an effect previously shown in vitro and in rodent models of disease. The IL-12 level was inversely correlated with NOS activity (r = −0.22; P = 0.007), with each twofold increase in NOS activity being predictive of a 25% (95% CI, 7 to 38%) decrease in plasma IL-12 levels. This probably reflects additional down-regulation of IL-12 by the high basal NO production and monocyte NOS expression found in the malaria-tolerant state. Neither the IL-12 level nor NOS activity was associated with either of two IL12B polymorphisms, reflecting the diversity of genetic control over immune responses in different populations.

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